KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Production of Gemcitabine-Loaded Poly (L-lactic acid) Microparticles Using Supercritical Carbon Dioxide: Effect of Process Parameters

초임계 이산화탄소를 이용한 Gemcitabine 함유 PLLA 미립자 제조: 공정 변수의 영향

  • Joo, Hyun-Jae (Department of Chemical Engineering, The University of Suwon) ;
  • Jung, In-Il (Department of Chemical Engineering, The University of Suwon) ;
  • Lim, Gio-Bin (Department of Chemical Engineering, The University of Suwon) ;
  • Ryu, Jong-Hoon (Department of Chemical Engineering, The University of Suwon)
  • Received : 2011.01.31
  • Accepted : 2011.02.21
  • Published : 2011.02.28
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Abstract

In this study, poly (L-lactic acid) (PLLA) microparticles containing gemcitabine hydrochloride were prepared by a supercritical fluid process, called aerosol solvent extraction system (ASES), utilizing supercritical carbon dioxide as antisolvent. The influence of process parameters such as temperature, pressure, $CO_2$ and solution flow rate, solution concentration, and feed ratio of drug to polymer on the morphology and characteristics of the microparticles was studied in detail. The gemcitabine-loaded microparticles exhibited a spherical shape with a smooth surface. The entrapment efficiency of gemcitabine increased with increasing temperature, solution concentration and $CO_2$ flow rate and with decreasing drug/polymer feed ratio. The maximum drug loading obtained from the ASES process was found to be about 11%. The ASES-processed PLLA microparticles containing gemcitabine showed a relatively high initial burst due to the presence of surface pores on the microparticles and the poor affinity between drug and polymer.

Keywords

References

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