Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Conservation of An Endangered Species of Lilium cernum Komarvo. through in vitro Mass-Propagation

멸종위기 종 솔나리의 기내 대량생산을 통한 보존 연구

  • Lee, Song-Hee (Department of Biological and Environmental Science College of Life Science and Biotechnology, Dongguk University) ;
  • Lee, Su-Gwang (Department of Biological and Environmental Science College of Life Science and Biotechnology, Dongguk University) ;
  • Kang, Ho-Duck (Department of Biological and Environmental Science College of Life Science and Biotechnology, Dongguk University)
  • 이송희 (동국대학교 바이오환경과학과) ;
  • 이수광 (동국대학교 바이오환경과학과) ;
  • 강호덕 (동국대학교 바이오환경과학과)
  • Received : 2010.12.10
  • Accepted : 2011.02.11
  • Published : 2011.02.28
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Abstract

This study was conducted to establish the optimal condition for conservation of genetic resources and micropropagation of Lilium cernum. Induction of bulbet of L. cernum was highly effective (9.2 bulb/explant) on 1/2 SH (Schenk and Hildebrandt) medium supplemented with 1.0 mg/L TDZ (Thidiazuron) and 0.1 mg/L NAA (Naphthaleneacetic acid). The treatment of 0.1 mg/L NAA increased root development (6.4 root/explant) under the in vitro condition. In addition, treatments of AC (Activated Charcoal) and ventilation were enhanced to develop number of shoots and to elongate length of leaf, bulb and root. Futhermore, the process of short-term soil acclimatization was promoted to strengthen the plantlets induced under the in vitro condition.

Keywords

References

  1. Bacchetta L, Remoti PC, Bernardini C and Saccardo F. (2003). Adventitious shoot regeneration from leaf explants and stem nodes of Lilium. Plant Cell Tissue Organ Culture. 74:37-44. https://doi.org/10.1023/A:1023315321931
  2. Bolar JP, Norelli JL, Aldwinckle HS and Hanke V. (1998). An efficient method for rooting and acclimation of micropropagated apple cultivars. Journal of American Society for Horticultural Science. 37:1251-1252.
  3. Cho WJ, Park HB and Choi EG. (1999). Effects of growth regulators and sucrose on plant differentiation and bulblet formation of Lilium cernum Komarov. Bulletin of the Agricultural College, Chonbuk National University. 30:22-32.
  4. Cournac L, Dimon B, Carrier P, Lohou A and Changvardieff P. (1991). Growth and photosynthetic characteristics of Solanum tuberosum plantlets cultivated in vitro in different conditions of aeration, sucrose supply, and $CO_2$ enrichment. Plant Physiology. 97:112-117. https://doi.org/10.1104/pp.97.1.112
  5. Debergh P. (1991). Acclimatization techniques of plants from in vitro. Acta-Horticulturae. 289:292-300
  6. Desjardins Y. (1995). Photosynthesis in vitro - on the factors regulating $CO_2$ enrichment and relative micropropagation system. Acta Horticulturae. 363:45-61.
  7. Dumas E and Monteuuis O. (1995). In vitro rooting of micropropagated shoots from juvenile and mature Pinus pinaster explants- influence of activated charcoal. Plant Cell, Tissue and Organ Culture. 40:231-235. https://doi.org/10.1007/BF00048128
  8. Eymar E, Alegre J, Toribio M and Lopez-vela D. (2000). Effect of activated charcoal and 6-benzyladenine on in vitro nitrogen uptake by Lagerstroemia indica. Plant Cell, Tissue and Organ Culture. 63:57-65. https://doi.org/10.1023/A:1006471519609
  9. Gabriela F, Carlos T, Carlos O, Yves D and Jorge MS. (2005). Exogenous sucrose can decrease in vitro photosynthesis but improve field survival and growth of coconut(Cocos nucifera L.) in vitro plantlets. In Vitro Cellular and Developmental Biology. 41:69-76. https://doi.org/10.1079/IVP2004597
  10. Islam MT, Dembele DP and Keller ERJ. (2005). Influence of explant, temperature and different culture vessels on in vitro culture for germplasm maintenance of four mint accessions. Plant Cell, Tissue and Organ Culture. 81:123-130. https://doi.org/10.1007/s11240-004-3307-3
  11. Jeong JH and Kim KS. (1991). Morphological Characteristics of Korean Native Lilies. Horticulture Environment and Biotechnology. 32:411-418.
  12. Jin YH, Park SH, Kim HH and Lee CH. (2001). Several Factors on in vitro bulblet production from native Lilium cernum and L. callosum leaf explants. The Plant Resources Society of Korea. 14:104-105.
  13. Kim HK, Lim JD, Hyun TK, Lee HY, Lee JH and Yu CY. (2001). In vitro culture and acclimatization of regenerated plants of Liliem cernum Komarov. Korean Journal of Medical Crop Science. 9:310-317.
  14. Kirdmanee C, Kitaya Y and Kozai T. (1995). Effect of $CO_2$ enrichment and supporting material in vitro on photoautotrophic growth of Eucalyptus plantlets in vitro and ex vitro. In vitro Cellular and Developmental Biology. 31:144-149. https://doi.org/10.1007/BF02632010
  15. Kozai T, Iwanami Y and Fujiwara K. (1987). Environment control for mass propagation of tissue culture plantlets. (1) Effets of $CO_2$ enrichment on the plantlet growth during the multiplication stage. Plant Tissue Culture Letters. 4:22-26. https://doi.org/10.5511/plantbiotechnology1984.4.22
  16. Lee CH and Kwon SJ. (2005). Effect of metal ions, antioxidants and dark treatments on plant regeneration of Dionaea muscipula. Journal of Korea Flower Researcher society. 13:201-206.
  17. Lee YM and Lee WY. (1997). Illustrated rare and endangered species in Korea. Korea National Arboretum. Po-cheon, Korea. p. 41.
  18. Lu CY. (1993). The use of thidiazuron in tissue culture. In Vitro Cellular and Developmental Biology. 29:92-96. https://doi.org/10.1007/BF02632259
  19. Magdalena K and Anna B. (2005). Morphogenesis of Lilium martagon L. explants in callus culture. Acta Biologia Cracoviensia Series Botanica. 47:65-73
  20. Matysiak B and Nowak J. (1998). Acclimatization and the grwoth Ficus benjamina microcuttings as affected by carbon dioxide concentration. Journal of Horticulture Science Biotechnology. 73:185-188. https://doi.org/10.1016/S0304-4238(97)00143-X
  21. Ministry of Environment (MEV). (2005). Enforcement decrees drafted for the Wildlife Protection Act. Gwacheon, Korea. p. 83.
  22. Ministry for Food, Agriculture, Forestry and Fisheries (MFAFF). (2004). Horticultural commercialization for pot plants by flowering promotion and establishment of the dwarfing culture of Korean native Lilium. Gwacheon, Korea. p. 20-22.
  23. Pan MJ and Van Staden J. (1998). The use of charcoal in in vitro culture-a review. Plant Growth Regulator. 26:155-163. https://doi.org/10.1023/A:1006119015972
  24. Peak KY, Yu KJ, Seong NS, Choi IS and Cho JT. (1994). Micropropagation through stem, node-bud shoot tip and bulbet scale culture in Fritillaria thunbergii Miq. Korean Journal of Medical Crop Science. 2:154-161.
  25. Schenk RU and Hildebrandt AC. (1972). Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures. Canadian Journal of Botany. 50:199-204. https://doi.org/10.1139/b72-026
  26. Seo JN, Kim HY, Lee SG and Kang HD. (2009). Effects of plant growth regulators on bulbets regeneration of Liliem cernum K. Journal of Agriculture and Life Science. 43:29-33.
  27. Shimizu M. (1987). The lilies of Japan (Japeneas), Species and hybrids. Seibundo Sinkou-sha. Tokyo, Japan. p. 7-12.
  28. Short KC and Torrey JG. (1972). Cytokinins in seedling roots of pea. Plant Physiology. 49:155-160. https://doi.org/10.1104/pp.49.2.155
  29. Takayama S and Misawa M. (1979). Differentiation in Lilium bulbscales grown in vitro. Effect of various cultural conditions. Physiology Plantarum 46:184-190. https://doi.org/10.1111/j.1399-3054.1979.tb06555.x
  30. Thomas TD. (2008). The role of activated charcoal in plant tissue culture. Biotechnology Advances. 26:618-631. https://doi.org/10.1016/j.biotechadv.2008.08.003
  31. Van Telgen HJ, Van Mil A and Kunneman B. (1992). Effect of propagation and rooting conditions on acclimatization of micropropagated plants. Acta botanica neerlandica. 41: 453-459. https://doi.org/10.1111/j.1438-8677.1992.tb00515.x
  32. Wang PJ and Huang LC. (1976). Beneficial effects of activated charcoal on plant tissue and organ cultures. In vitro. 12:260-262. https://doi.org/10.1007/BF02796447
  33. Zobayed SMA. (2005). Ventilation in micropropagation. In: T. Kozai et al. (eds). Photoautotrophic (sugar-free medium) micropropagation as a new propagation and transplant production system. Springer, Dordrecht, Netherlands. p. 147-186.

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