Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Distribution of Microorganisms in Perilla Leaf and Cultivation Area

들깻잎과 생산환경의 미생물 분포

  • Kim, Se-Ri (Microbial Safety Division, Department of Agro-Food Safety, NAAS, RDA) ;
  • Lee, Ji-Young (Microbial Safety Division, Department of Agro-Food Safety, NAAS, RDA) ;
  • Lee, Seo-Hyun (Microbial Safety Division, Department of Agro-Food Safety, NAAS, RDA) ;
  • Ko, Hyeon-Seok (Knowledge & Information Office, RDA) ;
  • Yoon, Yo-Han (Department of Food & Nutrition, Sookmyung Women's University) ;
  • Kwon, Se-Hyeok (Department of Statistics, Hannam University) ;
  • Ryu, Kyoung-Yul (Microbial Safety Division, Department of Agro-Food Safety, NAAS, RDA) ;
  • Yun, Hye-Jeong (Microbial Safety Division, Department of Agro-Food Safety, NAAS, RDA) ;
  • Kim, Won-Il (Microbial Safety Division, Department of Agro-Food Safety, NAAS, RDA) ;
  • Yun, Jong-Chul (Microbial Safety Division, Department of Agro-Food Safety, NAAS, RDA) ;
  • Kim, Doo-Ho (Administrator Office, RDA) ;
  • Chung, Duck-Hwa (Division of Applied Life Science, Gyeongsang National University)
  • 김세리 (농촌진흥청 국립농업과학원 농산물안전성부 유해생물과) ;
  • 이지영 (농촌진흥청 국립농업과학원 농산물안전성부 유해생물과) ;
  • 이서현 (농촌진흥청 국립농업과학원 농산물안전성부 유해생물과) ;
  • 고현석 (농촌진흥청 기획조정관 지식정보화담당관실) ;
  • 윤요한 (숙명여자대학교 생활과학부 식품영양학과) ;
  • 권세혁 (한남대학교 통계학과) ;
  • 류경열 (농촌진흥청 국립농업과학원 농산물안전성부 유해생물과) ;
  • 윤혜정 (농촌진흥청 국립농업과학원 농산물안전성부 유해생물과) ;
  • 김원일 (농촌진흥청 국립농업과학원 농산물안전성부 유해생물과) ;
  • 윤종철 (농촌진흥청 국립농업과학원 농산물안전성부 유해생물과) ;
  • 김두호 (농촌진흥청 청장실) ;
  • 정덕화 (경상대학교 응용생명과학부)
  • Received : 2010.09.27
  • Accepted : 2010.12.17
  • Published : 2011.04.30
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Abstract

The prevalence and distribution of hazardous microorganisms were investigated from the major perilla cultivation area at Milyang, Gyeongnam province, Korea. Aerobic plate count (APC) and coliform count of perilla leaves were 4.82 log CFU/g and 3.85 log CFU/g, respectively. E. coli, S. aureus and B. cereus were detected in 3.0% (4/114), 7.9% (9/114) and 46.5% (53/114) of examined perilla leaves. However, E. coli O157:H7, Salmonella spp, and L. monocytogenes were not detected. The distribution of hazardous microorganisms in perilla leaf cultivation environment were compared and the concentration of APC and coliform counts were more than 3.0 log CFU/(mL, g, $100cm^2$, hand) from most of the samples. S. aureus were detected from irrigation water, packing table, packing vinyl, hand, and clothes. Also, B. cereus was frequently detected from the examined samples. Especially, packing table and collection container were contaminated with maximum 5.5 log $CFU/100cm^2$ of B. cereus. Good Agricultural Practice (GAP) system should be introduced to farms to enhance the safety of perilla leaves.

본 연구는 국내 일일 평균 섭취량이 많은 들깻잎을 대상으로 미생물 안전성을 평가하고 미생물에 대한 오염도를 조사하였고, 검출빈도가 높은 S. aureus와 B. cereus의 오염원을 조사하였다. 들깻잎의 미생물 오염도 조사 결과 일반세균수 수준은 4.82 log CFU/g이었다. 대장균군의 오염수준은 0.83-5.33 log CFU/g으로 농가 간 차이가 크게 나타났으며 50% 농가의 들깻잎에서 4.00 log CFU/g 이상 검출되었다. 또한 병원성미생물 분석결과, E. coli는 3%(4/114)의 들깻잎에서 1.00 log CFU/g 이하로 검출되었으며 S. aureus의 경우 검출빈도는 7.9%(9/114)였고 1.6%(2/114)의 들깻잎에서 S. aureus가 2.00 log CFU/g 이상으로 다소 높게 검출되었다. B. cereus는 0-3.81 logCFU/g 수준이었고 검출빈도는 46.5%(53/114)였으며 2곳의 농가에서 3.00 log CFU/g 이상으로 높게 검출되었다. 한편 E. coli O157:H7, Salmonella spp, 그리고 L. monocytogenes는 모두 검출되지 않았다. 또한 들깻잎 생산환경(토양, 수질, 작업자 및 포장대 등)에서 위생지표세균과 S. aureus와 B. cereus를 조사한 결과 일반세균수와 대장균군이 대부분의 시료에서 3.00 log $CFU/100cm^2$ 이상 검출되었고 일부 손, 수확용기 및 포장대에서 대장균군이 4.00 log $CFU/100cm^2$, hand 이상으로 높게 검출되었다. S. aureus는 관개용수, 포장대, 포장비닐, 작업자의 손과 복장에서 검출되었고 검출 수준은 0.33-1.31 log $CFU/100cm^2$이었다. B. cereus의 경우는 다양한 시료에서 빈번하게 검출되었고 특히 앞서 위생지표세균결과에서 오염도가 높았던 수확용기와 포장대에서 최고 5.50 log $CFU/100cm^2$까지 검출되었으며 수확 전 들깻잎보다 수확 후 들깻잎에서 B. cereus 수준이 증가되는 경향을 보였다. 이상의 결과를 통하여 들깻잎의 유해미생물 오염은 생산단계부터 발생되고 있으므로 이러한 유해미생물의 오염을 사전에 관리하는 GAP제도를 도입해야 할 것이다.

Keywords

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