Food Science of Animal Resources (한국축산식품학회지)

Korean Society for Food Science of Animal Resources (한국축산식품학회)
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Effect of Hen Egg Supplementation on Blood Lipid Profile and Fecal Bile Acid of C57BL/6 Mouse Fed Normal and High Cholesterol Diet

계란 급여가 일반식이와 고콜레스테롤 식이를 급여한 C57BL/6 마우스의 혈중지질과 분변 담즙산에 미치는 영향

  • 장애라 (농촌진흥청 국립축산과학원) ;
  • 김동욱 (농촌진흥청 국립축산과학원) ;
  • 박정은 (농촌진흥청 국립축산과학원) ;
  • 최주희 (건국대학교 축산식품 생물공학과) ;
  • 강근호 (농촌진흥청 국립축산과학원) ;
  • 함준상 (농촌진흥청 국립축산과학원) ;
  • 오미화 (농촌진흥청 국립축산과학원) ;
  • 설국환 (농촌진흥청 국립축산과학원) ;
  • 이승규 (농촌진흥청 국립축산과학원) ;
  • 김동훈 (농촌진흥청 국립축산과학원) ;
  • 김현욱 (농촌진흥청 국립축산과학원) ;
  • 황경아 (농촌진흥청 국립농업과학원) ;
  • 황유진 (농촌진흥청 국립농업과학원) ;
  • 김혜경 (한서대학교 식품생물공학과)
  • Received : 2010.10.01
  • Accepted : 2011.02.22
  • Published : 2011.04.30
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Abstract

This study was conducted to evaluate the effect of whole egg supplementation on the blood lipid profiles and cholesterol levels of C57BL/6 mice. Sixty-six mice were divided into two groups: normal-diet supplemented and high-cholesterol diet supplemented. Lyophilized whole egg powder was mixed with the two diets at 2 and 10%: normal diet only, normal diet with 2 and 10% whole egg powder, high cholesterol diet only, high cholesterol diet with 2 and 10% whole egg powder. The mixed diets were fed for 5 wk and feeding condition (body weight change, feed intake, and feed efficiency ratio (FER)), blood lipid profiles (total cholesterol (TC), triglyceride (TG), high density lipoprotein-cholesterol (HDL-C), low density lipoprotein cholesterol, hepatic and fecal lipids (TG, TC)), and fecal bile acids were determined. No significant differences were found in body weight gain or FER after whole egg supplementation in both the normal and high-cholesterol diet fed groups. In the normal-diet fed mice, HDL-C increased significantly in the 2 and 10% whole-egg powder groups. In the high-cholesterol diet fed mice, administering 10% egg powder increased the atherogenic index compared to the control. Furthermore, administration of whole egg powder increased fecal bile acids dose dependently (p<0.05). These results indicate that administering 2% hen whole egg powder did not affect blood lipid profiles and was more beneficial for health by increasing HDL-C and aiding in the excretion of cholesterol by fecal bile acids than those in the control.

계란급여에 따른 혈중지질특성과 콜레스테롤 대사에 관한 영향을 조사하기 위해 C57BL/6 마우스를 공시하여 일반식이군과 고콜레스테롤식이군으로 나눈 후 계란을 2%와 10% 첨가한 사료를 5주동안 급여하였다. 일반사양성적(체중, 사료섭취량, 사료효율), 혈액과 간조직, 분변의 지질함량 및 담즙산 농도를 측정하였다. 체중과 사료효율은 일반식이와 고콜레스테롤식이군 모두 유의적인 차이가 없었으나 사료섭취량은 10% 계란 첨가구에서 유의적인 증가를 보였다(p<0.05). HDL-C농도는 일반식이 급여군에서 2%와 10% 계란 첨가구 모두 유의적인 증가를 나타내었다(p<0.05). 간 조직 내 TG와 TC농도는 일반식이군에서 계란의 급여가 TG와 TC 증가에 영향을 미친 반면 고콜레스테롤식이군의 경우 TG는 유의적인 차이가 없었고, TC는 10% 계란 급여군에서 감소하였다(p<0.05). 분변의 콜레스테롤, 중성지질, 담즙산 농도는 일반식이군과 고콜레스테롤식이군 모두 계란의 첨가 수준이 증가할수록 TC와 TG함량이 유의적으로 증가하였다(p<0.05). 담즙산 농도 역시 일반식이 급여군에서 계란의 첨가수준이 증가할수록 대조군보다 높은 함량을 보여 계란이 분변의 콜레스테롤 함량에도 영향을 미친 것으로 판단된다.

Keywords

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