Maxillofacial Plastic and Reconstructive Surgery

Korean Association of Maxillofacial Plastic and Reconstructive Surgeons (대한악안면성형재건외과학회)
권호 표지

Silk Fibroin and Substance P Combination Graft for the Reconstruction of a Bone Defect

실크 피브로인 지지체와 Substance P를 이용한 골 이식재

  • Park, Ki-Yu (Department of Dentistry, College of Medicine, Hallym University) ;
  • Choi, Kyo-Hee (Department of Dentistry, College of Medicine, Hallym University) ;
  • Park, Young-Ju (Department of Dentistry, College of Medicine, Hallym University) ;
  • Song, Ji-Young (Department of Oral and Maxillofacial Surgery, College of Dentistry, Gangneung-Wonju National University) ;
  • Kim, Seong-Gon (Department of Oral and Maxillofacial Surgery, College of Dentistry, Gangneung-Wonju National University) ;
  • Jo, You-Young (Sericultural & Apicultural Materials Division, National Academy of Agricultural Science, RDA) ;
  • Kweon, Hae-Yong (Sericultural & Apicultural Materials Division, National Academy of Agricultural Science, RDA) ;
  • Kang, Seok-Woo (Sericultural & Apicultural Materials Division, National Academy of Agricultural Science, RDA)
  • 박기유 (한림대학교 의학과 치과학교실) ;
  • 최교희 (한림대학교 의학과 치과학교실) ;
  • 박영주 (한림대학교 의학과 치과학교실) ;
  • 송지영 (강릉원주대학교 치과대학 구강악안면외과학교실) ;
  • 김성곤 (강릉원주대학교 치과대학 구강악안면외과학교실) ;
  • 조유영 (농촌진흥청) ;
  • 권해용 (농촌진흥청) ;
  • 강석우 (농촌진흥청)
  • Received : 2011.03.09
  • Accepted : 2011.04.10
  • Published : 2011.07.31
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Abstract

Purpose: Substance P is a well known neurotransmitter and has been known to mediate pain. Recently, it has been unveiled that substance P is involved in the recruitment of mesenchymal stem cells to wound sites. The purpose of this study was to exam bone formation when a combination of substance P and silk fibroin was used in a bone defect model. Methods: Twenty rabbits were used and 40 calvarial defects were formed. They were divided as 4 groups (unfilled control, silk only, silk+$10{\mu}g$/ml substance P; Sub10, and silk+$100{\mu}g$/ml substance P; Sub100). All animals were humanely sacrificed 4 or 8 weeks after grafting. The specimens were analyzed by micro-computerized tomography and histological analysis. Results: When compared to the unfilled control to silk only group, there was significant difference in bone mineral density (BMD) and the attenuation coefficient (AC) at 4 weeks ($p$=0.037 and 0.038, respectively). When compared Sub10 group to Sub100 group, there was significant difference in BMD and AC at 8 weeks ($p$=0.004 for all). Residual graft amounts were $52.1{\pm}15.8$%, $15.2{\pm}9.2$% and $9.0{\pm}3.3$% for silk only, Sub10, and Sub100 groups, respectively. When comparing the residual graft amount of silk only to sub10 or sub100, the differences were statistically significant ($p$ <0.001). Conclusion: The silk fibroin scaffold showed higher BMD and AC than the unfilled control. The combination graft with substance P and silk fibroin scaffold showed a faster graft degradation than with a silk fibroin scaffold only.

Keywords

References

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