The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology (한방안이비인후피부과학회지)

The Society of Korean Medicine Ophthalmology, Otolaryngology & Dermatology (대한한방안이비인후피부과학회)
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Effect of Anemarrhenae Rhizoma Ethanol Extract on Apoptosis Induction of HT-29 Human Colon Cancer Cells

지모(知母)에탄올추출물의 HT-29대장암세포 Apoptosis 유도효과

  • 김태현 (원광대학교 한의학 전문대학원 한약자원개발학과) ;
  • 김범호 (원광대학교 한의학 전문대학원 한약자원개발학과) ;
  • 전병국 (원광대학교 한의학 전문대학원 한약자원개발학과) ;
  • 윤정록 (원광대학교 한의학 전문대학원 한약자원개발학과) ;
  • 우원홍 (원광대학교 한의학 전문대학원 한약자원개발학과) ;
  • 문연자 (원광대학교 한의과 대학 해부학 교실) ;
  • 이장천 (부산대학교 한의학전문대학원 약물의학부) ;
  • 이부균 (세명대학교 한의과대학 본초방제학 교실) ;
  • 박영규 (원광대학교 교육개발센터) ;
  • 임규상 (원광대학교 한의학전문대학원, 한국 전통의학 연구소)
  • Received : 2011.02.21
  • Accepted : 2011.04.06
  • Published : 2011.04.25
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Abstract

Objective : In this study, we investigated the effects of ethanol extract of Anemarrhenae Rhizoma (EAR) on the proliferation and apoptosis induction of HT-29 human colon cancer cells. Methods : Cell viability of HT-29 cells were measured by MTT assay and apoptisis-related proteins were assessed using western blotting. Chromatin condensation of HT-29 cells stained with Hoechst 33258. Results : In the present study, we demonstrated that EAR exhibited significant cytotoxicity in HT-29 cells. The induction of apoptosis in HT-29 cells by EAR treatment was characterized by chromatin condensation and the activation of caspase-3. EAR-induced apoptosis is accompanied by the release of cytochrome c and the specific proteolytic cleavage of PARP. EAR was appeared cytotoxic effect to HT-29 cells in a dose-dependent manner. Concomitantly, EAR treatment led to increase in the caspase-9. The reduction of Bcl-2 and truncation of Bid were induced by EAR. Conclusion : We studied that the EAR induced apoptosis in human colon adenocarcinoma HT-29 cells. These results indicated that EAR can cause apoptosis through mitochondria/caspase pathway in human HT-29 cells.

Keywords

References

  1. Koh SJ, Kim JS. The reasons for the increased incidence of colorectal cancer in Korea. Korean J Med. 2010;79:97-103.
  2. Tenesa A, Dunlop MG. New insights into the aetiology of colorectal cancer from genome-wide association studies. Nat Rev Genet 2009;10:353-8.
  3. Terzic J, Grivennikov S, Karin E, Karin M. Inflammation and colon cancer. Gastroenterology. 2010;138:2101-14. https://doi.org/10.1053/j.gastro.2010.01.058
  4. Vazquez A, Bond EE, Levine AJ, Bond GL. The genetics of the p53 pathway, apoptosis and cancer therapy. Nat Rev Drug Discov. 2008;12:979-87.
  5. Heath-Engel HM, Chang NC, Shore GC. The endoplasmic reticulum in apoptosis and autophagy: role of the BCL-2 protein family. Oncogene. 2008;27(50):6419-33. https://doi.org/10.1038/onc.2008.309
  6. Pathak S, Sharma CH, Jayaram HN, Singh N. Apoptotic signaling induced by benzamide riboside : an in vitro study. Mol Cell Biochem. 2009;328(2):67-73. https://doi.org/10.1007/s11010-009-0075-8
  7. Wang S. The promise of cancer therapeutics targeting the TNF-related apoptosis-inducing ligand and TRAIL receptor pathway. Oncogene. 2008;27(48):6207-15. https://doi.org/10.1038/onc.2008.298
  8. Ashkenazi A. Targeting the extrinsic apoptosis pathway in cancer. Cytokine Growth Factor Rev. 2008;19(3-4):325-31. https://doi.org/10.1016/j.cytogfr.2008.04.001
  9. 김창민, 신민교, 안덕균, 이경순 외. 중약대사전. 서울:정담출판사. 1997:3951-57.
  10. 김현태, 곽용호, 성재덕, 김금숙, 박창기, 한상익. 지모의 유효성분 분리 및 HPLC 정량 분석. 한국약용작물학회지. 1999;7(1):45-50.
  11. 이정옥, 이승호, 유시용, 안종웅, 최상운, 노재성, 김성기. 지모의 항암활성성분에 관한 연구. 1995;26(1):47-50.
  12. 김영호. 대장암의 조기 발견 및 치료에 대한 전략 ; 대장암의 유전학적 관련성의 최신지견. 대한내과학회지. 2010;79(2):113-8.
  13. 중앙암등록본부. 2007년 국가암등록사업 연례 보고서.
  14. Williams GT. Programmed cell death: apoptosis and oncogenesis. Cell, 1991;65(7):1097-8. https://doi.org/10.1016/0092-8674(91)90002-G
  15. 21. Levine B, Sinha S, Kroemer G. Bcl-2 family members: dual regulators of apoptosis and autophagy. Autophagy. 2008;4(5):600-6. https://doi.org/10.4161/auto.6260
  16. Suen DF, Norris KL, Youle RJ. Mitochondrial dynamics and apoptosis. Genes Dev. 2008;22(12):1577-90. https://doi.org/10.1101/gad.1658508
  17. Nemec KN, Khaled AR. Therapeutic modulation of apoptosis: targeting the BCL-2 family at the interface of the mitochondrial membrane. Yonsei Med J. 2008;49(5):689-97. https://doi.org/10.3349/ymj.2008.49.5.689
  18. 김진우, 조철오. Apoptosis의 분자적 특징과 조절 기작. Ajou Med J. 1998;3(1):1-11.
  19. Tsang WP, Kwok TT. Let-7a microRNA suppresses therapeutics-induced cancer cell death by targeting caspase-3. Apoptosis. 2008;13(10):1215-22. https://doi.org/10.1007/s10495-008-0256-z
  20. Kim R, Emi M, Tanabe K. Caspase-dependent and -independent cell death pathways after DNA damage. Oncol Rep. 2005;14(3):595-9.
  21. Selvakumar P, Sharma RK. Role of calpain and caspase system in the regulation of N-myristoyltransferase in human colon cancer. Int J Mol Med. 2007;19(5):823-7.
  22. Chai F, Truong-Tran AQ, Ho LH, Zalewski PD. Regulation of caspase activation and apoptosis by cellular zinc fluxes and zinc deprivation. Immunol Cell Biol. 1999;77(3):272-8. https://doi.org/10.1046/j.1440-1711.1999.00825.x
  23. Ashkenazi A, Dixit VM. Death receptors: signaling and modulation. Science. 1998;281(5381):1305-8. https://doi.org/10.1126/science.281.5381.1305
  24. Budihardjo I, Oliver H, Lutter M, Luo X, Wang X. Biochemical pathways of caspase activation during apoptosis. Annu Rev Cell Dev Biol. 1999;(15):269-90.
  25. Eeva J, Nuutinen U, Ropponen A, Matto M, Eray M, Pellinen R, Wahlfors J, Pelkonen J. The involvement of mitochondria and the caspase-9 activation pathway in rituximab-induced apoptosis in FL cells. Apoptosis. 2009;14(5):687-98. https://doi.org/10.1007/s10495-009-0337-7