Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
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Effect of Myadis Stigma Water Extract on Adipogenesis and Blood Glucose in 3T3-L1 Adipocytes and db/db Mice

3T3-L1 지방세포와 db/db 마우스에서 옥수수수염 물 추출물의 지방생성 억제 및 혈당 저하효과

  • Min, Oh-Jin (Dept. of Oriental Medicine Resources, Mokpo National University) ;
  • Sharma, Bhesh Raj (Dept. of Oriental Medicine Resources, Mokpo National University) ;
  • Park, Chul-Min (Dept. of Oriental Medicine Resources, Mokpo National University) ;
  • Rhyu, Dong-Young (Dept. of Oriental Medicine Resources, Mokpo National University)
  • Received : 2011.05.23
  • Accepted : 2011.06.07
  • Published : 2011.06.30
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Abstract

Obesity occur from the imbalance between energy intake and energy expenditure. Obesity is a complex chronic disease that is suggested to cause other metabolic disorders such as type 2 diabetes, hyperlipidemia, hypertension, and arteriosclerosis. In this study, our purpose is to investigate the anti-hyperglycemic and anti-obesitic effects of Maydis stigma water extract in 3T3-L1 adipocytes and db/db mice. Maydis stigma water extract at dose of 100 and 500 ${\mu}g/ml$ slowly inhibited cell viability as compared to that of control in mature adipocytes. Also, the additions of 50 and 250 ${\mu}g/ml$ of Maydis stigma water extract significantly inhibited the lipid accumulations and CCAAT/enhancer-binding protein(C/EBP) ${\alpha}$ and peroxisome proliferator-activated receptor(PPAR) ${\gamma}$ expressions with dose-dependent manner in 3T3-L1 adipocytes. Maydis stigma water extract at 250, 500, and 1000 ${\mu}g/ml$ only showed the increasing pattern on lipolysis activity. The oral treatment of Maydis stigma water extract (100 or 400 mg/kg body weight) in db/db mice only showed tendency to decrease body weight, food efficiency ratio (FER), HbA1c, blood glucose, total cholesterol, triglyceride, and the adipocyte size of in db/db mice. However, Maydis stigma water extract increased the insulin level in a dose dependent manner. Thus these results indicate that Maydis stigma water extracxt inhibits adipogenesis through regulation of C/EBP${\alpha}$ and PPAR${\gamma}$ expressions in 3T3-L1 adipocytes and shows anti-hyperglycemic effect through increase of insulin secretion in db/db mice.

Keywords

References

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