Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Development of SCAR Markers for the Authentication of Acori Rhizoma Based on the Analysis of RAPD and Multiplex-PCR

RAPD 분석과 multiplex-PCR을 이용한 석창포 감별용 SCAR 마커 개발

  • Moon, Byeong-Cheol (Center of Herbal Resources Research, Korea Institute of Oriental Medicine) ;
  • Ji, Yun-Ui (Center of Herbal Resources Research, Korea Institute of Oriental Medicine) ;
  • Lee, Young-Mi (Center of Herbal Resources Research, Korea Institute of Oriental Medicine) ;
  • Chun, Jin-Mi (Center of Herbal Resources Research, Korea Institute of Oriental Medicine) ;
  • Lee, A-Yeong (Center of Herbal Resources Research, Korea Institute of Oriental Medicine) ;
  • Choo, Byung-Kil (College of Agriculture and Life Science, Chonbuk National University) ;
  • Kim, Ho-Kyoung (Center of Herbal Resources Research, Korea Institute of Oriental Medicine)
  • 문병철 (한국한의학연구원 한약자원연구센터) ;
  • 지윤의 (한국한의학연구원 한약자원연구센터) ;
  • 이영미 (한국한의학연구원 한약자원연구센터) ;
  • 천진미 (한국한의학연구원 한약자원연구센터) ;
  • 이아영 (한국한의학연구원 한약자원연구센터) ;
  • 추병길 (전북대학교 농업생명과학대학) ;
  • 김호경 (한국한의학연구원 한약자원연구센터)
  • Received : 2011.05.13
  • Accepted : 2011.06.16
  • Published : 2011.06.30
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Abstract

The rhizomes and herbal medicines originating from Acorus gramineus, A. calamus, A. tatarinowii, and A. gramineus var. pusilus, show significant similarity, and the correct identification of species is very difficult. Random Amplified Polymorphic DNA (RAPD) and Sequence Characterized Amplified Region (SCAR) were used to develop a reliable method for identification of these four species. Several distinct SCAR markers were developed from species-specific RAPD amplicons for each species. Furthermore, a useful molecular marker was established for multiplex-PCR, in order to the four species could be distinguished concurrently. These markers allow efficient and rapid identification of closely-related Acorus species and will be useful for standardization of herbal medicines.

Keywords

References

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