Natural Product Sciences

The Korean Society of Pharmacognosy (한국생약학회)
권호 표지

A Convenient HPLC/ELSD Method for the Quantitative Analysis of Betaine in Lycium chinense

  • Received : 2011.04.16
  • Accepted : 2011.05.18
  • Published : 2011.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

In order to facilitate the quality control of betaine from the fruits of Lycium chinense, we have developed a rapid and simple method for quantitative determination. Determination was achieved on a Discovery C18 column with an isocratic solvent system of 0.32% perfluoropentanoic acid aqueous-acetonitrile at a flow-rate of 0.5 mL/min and detected an ELSD. The method was reproducible with intra- and inter-day variations of less than 6% (R.S.D). The recoveries were in the range of 90.01~100.05%. The method turned out to be fast and simple, furthermore, to have a good selectivity and sensitivity for the quantity determination of betaine in the fruits of L.chinense.

Keywords

References

  1. Barak, A.J., Harriet, C., Beckenhauer, H.C., Matti, J., and Dean, J.T., Dietary Betaine Promotes Generation of Hepatic SAdenosylmethionine and Protects the Liver from Ethanol-Induced Fatty Infiltration. Alcohol. Clin. Experiment. Res. 17, 552-555 (1993). https://doi.org/10.1111/j.1530-0277.1993.tb00798.x
  2. Funayama, S., Yoshida, K., Konno, C., and Hikino, H., Structure of kukoamine A, a hypotensive principle of Lycium chinense root barks1. Tetrahedron Lett. 21, 1355-1356 (1980). https://doi.org/10.1016/S0040-4039(00)74574-6
  3. Gahl, W.A., Bernardini, I., and Chen, S., The effect of oral betaine on vertebral body bone density in pyridoxine-nonresponsive homocystinuria. J. Inherit. Metabol. Dis. 11, 291-298 (1988). https://doi.org/10.1007/BF01800372
  4. Junnila, M., Barak, A.J., Beakenhauer, H.C., and Rahko, T., Betaine reduces hepatic lipidosis induced by carbon tetrachloride in Sprague- Dawley rats. Veter. Human Toxicol. 40, 263-266 (1998).
  5. Kikuchi, N., Matsuno, K., and Miki, K., Separation and determination of betaine in an oriental medicine by liquid chromatography. Analytica Chimica Acta 283, 338-343 (1993). https://doi.org/10.1016/0003-2670(93)85241-B
  6. Murakami, T., Nagamura, Y., and Hirano, K., The Recovering Effect of Betaine on Carbon Tetrachloride-Induced Liver Injury. J. Nutrit. Sci. Vitaminol. 44, 249-255 (1998). https://doi.org/10.3177/jnsv.44.249
  7. Mar, M.H., Ridky, T.W., Garner, S.C., and Ziesel, S.H., A method for the determination of betaine in tissues using high performance liquid chromatography. J. Nutrit. Biochem. 6, 392-398 (1995). https://doi.org/10.1016/0955-2863(95)80008-Z
  8. Noguchi, M., Mochida, K., Shingu, T., Kozuka, M., and Fujitani, K., Uber die Bestandteile der chinesischen Droge "Ti-ku-'pi."I. Isolierung und Konstitution von Lyciumamid, einem neuen Dipeptid. Chem. Pharm. Bull. 32, 3584-3587 (1984). https://doi.org/10.1248/cpb.32.3584
  9. Selhub, J., Homocysteine metabolism. Ann. Rev. Nutrit. 19, 217-246 (1999). https://doi.org/10.1146/annurev.nutr.19.1.217
  10. Shin, Y.G., Cho, K.H., Kim, J.M., Park, M.K., and Park, J.H., Determination of betaine in Lycium chinense fruits by liquid chromatography-electrospray ionization mass spectrometry. J. Chromatograph. A 857, 331-335 (1999). https://doi.org/10.1016/S0021-9673(99)00720-7
  11. Sannai, A., Fujimori, T., and Kato, K., Neutral Volatile Components of "Kukoshi" (Lycium chinense M.). Agricul. Biol. Chem. 47, 2397-2399, (1983). https://doi.org/10.1271/bbb1961.47.2397
  12. Wendel, U. and Bremer, H.J., Betaine in the treatment of homocystinuria due to 5,10-methylenetetrahydrofolate reductase deficiency. Eur. J. Pediatrics 142, 147-150 (1984). https://doi.org/10.1007/BF00445602
  13. Yang, L.L., Yen, K.Y., Kiso, Y., and Kikino, H., Antihepatotoxic actions of formosan plant drugs. J. Ethnopharmacol. 19, 103-110 (1987). https://doi.org/10.1016/0378-8741(87)90142-5