Herbal Formula Science (대한한의학방제학회지)

The Korean Medicine Society for the Herbal Formula Study (대한한의학방제학회)
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Anti-inflammatory Effect of Gagam-GongJin-dan in mouse peritoneal macrophages

마우스 복강대식세포에서 가감공진단(加減拱辰丹)의 항염증 효과

  • Kim, Hong-Jun (Dept. of Oriental Medical Prescription, Woo-Suk University) ;
  • Kim, Young-Sik (Dept. of Oriental Medical Prescription, Woo-Suk University) ;
  • Mok, Ji-Ye (Jeon-Ju University Atopy & Health Research Institute) ;
  • Jeong, Seung-Il (Jeon-Ju Biomaterials Institute) ;
  • Hwang, Sung-Yeoun (Dept. of Oriental Medical Prescription, Woo-Suk University) ;
  • Cho, Jung-Keun (School of Alternative Medicine & Health Science, College of Alternative Medicine, Jeon-Ju University) ;
  • Jang, Seon-Il (Jeon-Ju University Atopy & Health Research Institute)
  • 김홍준 (우석대학교 한의과대학 본초방제학교실) ;
  • 김영식 (우석대학교 한의과대학 본초방제학교실) ;
  • 목지예 (전주대학교 아토피.건강전문연구소) ;
  • 정승일 (전주생물소재연구소) ;
  • 황성연 (우석대학교 한의과대학 본초방제학교실) ;
  • 조정근 (전주대학교 대체의학대학 대체건강관리학부) ;
  • 장선일 (전주대학교 아토피.건강전문연구소)
  • Received : 2011.05.20
  • Accepted : 2011.06.13
  • Published : 2011.06.30
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Abstract

Objectives : In a previous study, we have shown that Gagam-Gongjin-Dan(GGD) has an inhibitory effect on the ovalbumin-induced immune responses and a hepatoprotective effect on actaminophen-induced liver injury in Balb/c Mice. However, the possible anti-inflammatory effect of GGD extract for inflammatory mediators was not reported. Therefore, the purpose of this study was to investigate an inhibitory effects of GGD extract against lipopolysaccharides(LPS) induced inflammatory mediators in mouse peritoneal macrophages. Methods : GGD extract was prepared by extracting with methanol for 7 days. The extract was freeze-dried following filtration through vacuum distillation system. Accumulated nitrite, an oxidative product of nitric oxide(NO), was measured in the culture medium by the Griess reaction. The levels of prostaglandin $E_2(PGE_2)$, interleukin-$1{\beta}$(IL-$1{\beta}$), tumor necrosis factor-${\alpha}$(TNF-${\alpha}$) were measured by enzyme-linked immunosorbent assay. The expressions of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2(COX-2) were measured by Western blot analysis. Results : GGD extract (50-$400\;{\mu}g$/ml) per se had no cytotoxic effect in LPS-stimulated peritoneal macrophages. GGD extract dose-dependently reduced NO, $PGE_2$, IL-$1{\beta}$ and TNF-${\alpha}$ production and COX-2 activity caused by stimulation of LPS. The levels of iNOS and COX-2 protein expressions were markedly suppressed by the treatment with GGD extract in a dose dependent manner. Conclusions : These results suggest that GGD extract has an anti-inflammatory effect against LPS-induced inflammatory mediators in peritoneal macrophages, these properties may contribute to inflammation disease care.

Keywords

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