Herbal Formula Science (대한한의학방제학회지)

The Korean Medicine Society for the Herbal Formula Study (대한한의학방제학회)
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Ethyl acetate fraction of GGEx18 modulates feeding efficiency ratio and blood leptin level in high fat diet-fed obese mice

GGEx18의 ethyl acetate 분획물에 의한 고지방식이 비만 마우스의 식이효율과 혈중 leptin 농도에 미치는 영향

  • Park, Ki-Jeong (Dept. of Formula Science, College of Oriental Medicine, Dong-Eui University) ;
  • Lee, Hee-Young (Dept. of Formula Science, College of Oriental Medicine, Dong-Eui University) ;
  • Lee, Hye-Rim (Dept. of Formula Science, College of Oriental Medicine, Dong-Eui University) ;
  • Yoon, Mi-Chung (Dept. of Life Sciences, Mok-Won University) ;
  • Park, Sun-Dong (Dept. of Formula Science, College of Oriental Medicine, Dong-Guk University) ;
  • Lee, Yong-Tae (Dept. of Physiology, College of Oriental Medicine & Research Institute of Oriental Medicine, Dong-Eui University) ;
  • Shen, Zhi-Bin (Dept. of Chemistry and Analysis of Traditional Chinese Medicine, College of Chinese Materia Medica, Guangdong Pharmaceutical University) ;
  • Cui, Hong-Hua (Dept. of Chemistry and Analysis of Traditional Chinese Medicine, College of Chinese Materia Medica, Guangdong Pharmaceutical University) ;
  • Shin, Soon-Shik (Dept. of Formula Science, College of Oriental Medicine, Dong-Eui University)
  • 박기정 (동의대학교 한의과대학 방제학교실) ;
  • 이희영 (동의대학교 한의과대학 방제학교실) ;
  • 이혜림 (동의대학교 한의과대학 방제학교실) ;
  • 윤미정 (목원대학교 바이오건강학부) ;
  • 박선동 (동국대학교 한의과대학 방제학교실) ;
  • 이용태 (동의대학교 한의과대학 생리학교실 및 한의학연구소) ;
  • 심지빈 (광동약학원 중약학원 중약화학여분석계) ;
  • 최홍화 (광동약학원 중약학원 중약화학여분석계) ;
  • 신순식 (동의대학교 한의과대학 방제학교실)
  • Received : 2011.05.20
  • Accepted : 2011.06.13
  • Published : 2011.06.30
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Abstract

Objectives : This study was designed to determine the effects of the GGEx18 ethyl acetate fraction(EF) on body weight gain, feeding efficiency ratio, and obesity-related factors in plasma as well as histology of liver and adipose tissues using high fat diet-fed male C57BL/6N obese mice. Methods : 8 weeks old, high fat diet-fed obese male mice were divided into 5 groups: C57BL/6N normal, control, EF(1), EF(2) and EF(3). After mice were treated with EF for 9 weeks, we measured body weight gain, food intake, feeding efficiency ratio, fat weight, plasma leptin and lipid levels. We also analysed histology of liver and adipose tissues on high fat diet-fed male C57BL/6N obese mice. Results : Compared with control, EF-treated mice had significantly lower body weight gain and feeding efficiency ratio. Consistent with the effects on body weight gain, EF significantly decreased the adipose tissue weight compared with control. Consistent with the effects on feeding efficiency ratio, EF significantly decreased plasma leptin concentrations compared with control. EF reduced the size of adipocytes as well as hepatic lipid accumulation compared with control. EF seems to be safe since not only the plasma levels of ALT and AST are within the normal range, but also EF did not show any toxic effects on organs. EF(3) was most effective among EF(1), EF(2), and EF(3) at doses of 25, 50, and 100 mg/kg, respectively. Conclusions : These results demonstrate that EF effectively reduces body weight gain, feeding efficiency ratio in high fat diet-fed obese mice, leading to the modulation of obesity. In addition, EF decreases the size of adipocytes and improves plasma lipids and controls hepatic lipid accumulation, suggesting that EF may act as a therapeutic agent for obesity.

Keywords

Acknowledgement

Supported by : 동의대학교

References

  1. Speakman JR, Selman C. Physical activity and resting metabolic rate. Proc Nutr Soc. 2003;62(3):621-34. https://doi.org/10.1079/PNS2003282
  2. Kim DK, Park HW. Relationship between resting metabolic rate and VO2 max in obese women. J Kor Sports Med. 2005;23(2):131-6.
  3. Park HS. Pathophysiology of obesity and energy control for obesity treatment. J Korean Acad Fam Med. 2000;22(8):1287-95.
  4. Korean Ministry of Health and Welfare. The Third Korea National Health and Nutrition Examination Survey. 2005.
  5. 한의과대학 본초학 편찬위원회. 본초학. 초판 발행. 서울 : 영림사. 2004:154-6, 285-7, 510-1.
  6. 尹琦現. Ob/Ob 마우스에서 GGEx18의 비만 조절 지방산 산화효소 유전자 발현 촉진. 東義大學校大學院. 韓醫學博士學位論文. 2009:12-65.
  7. 楊裕仁. 고지방식이 비만마우스에서 GGEx18이 체중감량과 PPAR alpha 표적유전자 발현에 미치는 영향. 東義大學校大學院. 韓醫學博士學位論文. 2009:8-50.
  8. 車昌敏. C2C12 골격근세표에서 GGEx18에 의한 지방산 $\beta$-산화효소 유전자발현의 조절. 東義大學校大學院. 韓醫學博士學位論文. 2009:9-21.
  9. 오창호. 비만억제자인 $\alpha$-MSH 유사체의 합성 및 약효분석 (신약개발연구개발사업 최종보고서). 서울:보건복지부. 2003:21.
  10. Garrido-Polonio C,Garcia-Linares MC, Garcia-Arias MT, Lopez-Varela S, Garcia-Fernandez MC, Terpstra AH, Sanchez- Muniz FJ.. Thermally oxidised sunflower-seed oil increases liver and serum peroxidation and modifies lipoprotein composition in rats. Br J Nutr. 2004. Aug;92(2):257-65. https://doi.org/10.1079/BJN20041174