Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Mutagenic and Antimutagenic Effects of Hemp Seed Oil Evaluated by Ames Salmonella Testing

삼종실유의 항돌연변이 효과

  • Jeun, Jung-Ae (Division of Food Bioscience & Technology, College of Life Sciences and Biotechnology, Korea University) ;
  • Cho, Hee-Joon (Division of Food Bioscience & Technology, College of Life Sciences and Biotechnology, Korea University) ;
  • Jun, Hee-Jin (Division of Food Bioscience & Technology, College of Life Sciences and Biotechnology, Korea University) ;
  • Lee, Ji-Hae (Division of Food Bioscience & Technology, College of Life Sciences and Biotechnology, Korea University) ;
  • Jia, Yao-Yao (Division of Food Bioscience & Technology, College of Life Sciences and Biotechnology, Korea University) ;
  • Cho, Kyoung-Sang (Department of Biological Science, Konkuk University) ;
  • Kim, Eun-Soo (Department of Biological Science, Konkuk University) ;
  • Lee, Sung-Joon (Division of Food Bioscience & Technology, College of Life Sciences and Biotechnology, Korea University)
  • Received : 2011.02.24
  • Accepted : 2011.03.06
  • Published : 2011.06.30
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Abstract

We examined the in vitro mutagenic and antimutagenic effects of hexane-extracted hemp (Cannabis sativa L. subsp. sativa var. sativa) seed oil (HO) with and without S9-mediated metabolic activation, using the TA98 and TA100 Salmonella Typhimurium strains. The MTT assay revealed no cytotoxicity in HepG2 cells for HO quantities $\leq400g$/mL. In the mutagenicity test, revertant colonies did not exceed spontaneous colonies in number. Colony numbers did not increase in either strain after HO treatment, with or without metabolic activation. HO showed no mutagenic effects and did not induce a mutation in either strain. In the antimutagenicity test, HO reduced the number of mutated colonies induced by 4NQO in both strains. The inhibition rates of HO (TA98, 21-91%; TA100, 21-85%) indicated a potent reduction in mutagenicity induced by 4NQO. HO showed no significant mutagenicity and may have antimutagenic effects, as assessed by Ames testing.

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References

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