Journal of Acupuncture Research

Korean Acupuncture & Moxibustion Medicine Society (대한침구의학회)
권호 표지

Effect of Paeoniae Radix Rubra Extract on the Production of NO and Prostaglandin $E_2$ in LPS-stimulated RAW264.7 Marcrophages

적작약(赤芍藥) 추출물이 대식세포(大食細胞)에서 NO 및 $PGE_2$ 생성에 미치는 영향

  • Han, Sang-Yeob (Dept. of Acupuncture & Moxibustion, Bucheon Jaseng Hospital of Oriental Medicine) ;
  • Lee, Eun-Yong (Dept. of Acupuncture & Moxibustion, College of Oriental medicine, Semyung University)
  • 한상엽 (부천자생한방병원 침구과) ;
  • 이은용 (세명대학교 한의과대학 침구학교실)
  • Received : 2011.01.18
  • Accepted : 2011.01.26
  • Published : 2011.02.20
Download PDF
⟨ Previous Next ⟩

Abstract

Objectives : The objective of this study is to study the effects of hot aqueous extract and ethanol extract from Paeoniae Radix Rubra on nitric oxide(NO) and prostaglandin $E_2(PGE_2)$ production in macrophage. Methods : Paeoniae Radix Rubra were extracted in 2 ways. one was extracted with hot aqueous for 4 hr in $100^{\circ}C$ and the other one was extracted with 70% ethanol for 4 hr in $70^{\circ}C$. RAW264.7 cells, a mouse macrophage lines, were incubated with different concentrations of the extract for 30 min and then stimulated with LPS at indicated times. Cell toxicity was determined by MTT assay. The concentrations of NO and $PGE_2$ were measured by griess assay and enzyme immunoassay(EIA). Results : The hot aqueous and ethanol extracts of Paeoniae Radix Rubra significantly inhibited the NO productions in LPS-stimulated RAW264.7 cells. The hot water extract of Paeoniae Radix Rubra significantly inhibited the $PGE_2$ productions in LPS-stimulated RAW264.7 cells. Conclusions : Our results demonstrated that Paeoniae Radix Rubra extract is able to significantly inhibit the production of NO, $PGE_2$ expression. Hot aqueous extract of Paeoniae Radix Rubra has more effective anti-inflammation than ethanol extract.

Keywords

References

  1. 전국한의과대학 본초학교수 공편저. 본초학. 서울 : 영림사. 1991 : 195.
  2. 안덕균. 한국본초도감. 서울 : 교학사. 2003 : 713.
  3. Shu YZ, Hatori M, Akao T, Kobashi K, Kagei K, Fukuyama K, Tsukihara T and Namba T. Metabolism of paeoniflorin and related compounds by human intestinal bacteria. Chem. Pharm. Bull. 1987 ; 35 : 3726. https://doi.org/10.1248/cpb.35.3726
  4. Bogdan, C. Nitric oxide and the immune response. Nat Immunol. 2001 ; 2(10) : 907-16. https://doi.org/10.1038/ni1001-907
  5. Chang YH, Lee ST, Lin WW. Effects of cannabinoids on LPS-stimulated inflammatory mediator release from macrophages:involvement of eicosanoids. J Cell Biochem. 2001 ; 81(4) : 715-23. https://doi.org/10.1002/jcb.1103
  6. Korhonen R, Lahti A, Kankaanranta H, Moilanen E. Nitric oxide production and signaling in inflammation. Curr Drug Targets Inflamm Allergy. 005 ; 4(4) : 471-9. https://doi.org/10.2174/1568010054526359
  7. Raison CL, Capuron L, Miller AH. Cytokines sing the blues: inflammation and the pathogenesis of depression. Trends Immunol. 2006 ; 27(1) : 24-31. https://doi.org/10.1016/j.it.2005.11.006
  8. 이상인, 김동걸, 노승현, 이영종, 주영승. 방제학. 서울 : 영림사. 1992 : 29-36.
  9. 리상인. 새로운 한약제제의 개발에 관한연구(-淸熱解毒湯 忍冬藤의 제형화에 관한연구-)보고서. 한국한의학연구원. 1998 : 106-7.
  10. 김유승, 류봉하, 김진성. 元氣生脈散의 추출물별 항산화작용과 쥐의 운동피로 회복효과. 대한한방내과학회. 2009 ; 30(1) : 94-106.
  11. 허태석, 고은정, 정희재, 이형구, 배현수, 정승기. 加味淸上補下湯의 물과 에탄올 추출법에 따른 抗알레르기 效果에 對한 硏究. 대한한방내과학회지. 2006 ; 27(1) : 40-54.
  12. Mosman T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. Journal of immunologic methods. 1983 ; 65(1-2) : 55-63.
  13. 허준. 동의보감. 서울 : 동의보감출판사. 2005 : 2171.
  14. Guo LY, Hung TM, Bae KH, Shin EM, Zhou HY, Hong YN, Kang SS, Kim HP, Kim YS. Anti-inflammatory effects of schisandrin isolated from the fruit of Schisandra chinensis Baill. European journal of pharmacology. 2008 ; 591 (1-3) : 293-9.
  15. Bosca L, Zeini M, Traves PG, Hortelano S. Nitric oxide and cell viability in inflammatory cells: a role for NO in macrophage function and fate. Toxicology. 2005 ; 208(2) : 249-58. https://doi.org/10.1016/j.tox.2004.11.035
  16. Nathan C. Nitric oxide as a secretory product of mammalian cells. The FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 1992 ; 6(12) : 3051-64.
  17. 염정호. 니켈 및 코발트의 세포독성 기전에서 NO의 역할. 대한산업의학회지. 2001 ; 13(3) : 274-7.
  18. 김창민, 박용기. 강활의 RAW264.7 세포에서 LPS에 의해 유도되는 염증물질 생성에 대한 효과. 대한본초학회지. 2009 ; 24(1) : 170-7.
  19. 윤용갑, 김규민, 이성준, 유승훈, 장선일. 금은화 수용성 추출물의 LPS 유도 염증매개물 억제 효과. 대한본초학회지. 2007 ; 22(3) : 118-23.
  20. 곡정강, 정승기, 정희재, 김진주. 連翹의 LPS로 유도된 Raw 264.7 Cell에서의 抗炎症 효과. 대한한방내과학회지. 2010 ; 31(2) : 54-63.
  21. 오성원, 김병우. 土茯苓의 RAW 264.7 세포에 대한 항염효과. 대한한방내과학회지. 2009 ; 30(2) : 288-95.
  22. 김현수, 김연숙, 정준희, 김이화. 황기추출물이 LPS로 염증유도된 RAW 264.7 대식세포주에서 산화질소 및 프로스타글란딘 생성에 미치는 영향. 세명대학교 한의학연구소 논문집. 2007 ; 10(10) : 45- 55.
  23. Hobbs AJ, Higgs A, Moncada S. Inhibition of nitric oxide synthase as a potential therapeutic target. Annu Rev Pharmacol Toxicol. 1999 ; 39 : 191-220. https://doi.org/10.1146/annurev.pharmtox.39.1.191
  24. Hibbs JB Jr, Taintor RR, Vavrin Z, Rachlin EM. Nitric oxide: acytotoxic activates macrophage effector molecule. Biochem. Biophys Res Commun. 1988 ; 157(1) : 87-94. https://doi.org/10.1016/S0006-291X(88)80015-9
  25. Wang Y, Marsden PA. Nitric oxide synthases: gene structure and regulation. Adv Pharmacol. 1995 ; 34 : 71-90.
  26. Duerksen-Hughes PJ, Day DB, Laster SM, Zachariades NA, Aquino L, Gooding LR. Both tumor necrosis factor and nitric oxide participate in lysis of simian virus 40-transformed cells by activated macrophages. J Immunol. 1992 ; 149(6) : 2114-22.
  27. Kuo CL, Chi CW, Liu TY. The anti-inflammatory potential of berberine in vitro and in vivo. Cancer letters. 2004 ; 203 : 127-37. https://doi.org/10.1016/j.canlet.2003.09.002
  28. Lee DU, Kang YJ, Park MK, Lee YS, Seo HG, Kim TS, Kim CH, Chang KC. Effects of 13-alkyl-substituted berberine alkaloids on the expression of COX-II, TNF-alpha, iNOS, and IL-12 production in LPS-stimulated macrophages. Life sciences. 2003 ; 73 : 1401-12. https://doi.org/10.1016/S0024-3205(03)00435-1
  29. Kim KW, Ha KT, Park CS, Jin UH, Chang HW, Lee IS, Kim CH. Polygonum cuspidatum, compared with baicalin and berberine, inhibits inducible nitric oxide synthase and cyclooxygenase- 2 gene expressions in RAW 264.7 macrophages. Vascular pharmacology. 2007 ; 47 : 99-107. https://doi.org/10.1016/j.vph.2007.04.007
  30. Park JY, Pillinger MH, Abramson SB. Prostaglandin E2 synthesis and secretion: the role of $PGE_2$ synthases. Clinical immunology(Orlando, Fla). 2006 ; 119(3) : 229-40.