Journal of the korean academy of Pediatric Dentistry (대한소아치과학회지)

Korean Academy of Pediatric Dentistry (대한소아치과학회)
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PERIODONTOPATHIC BACTERIA AND ANTIBIOTIC RESISTANCE GENES OF ORAL BIOFILMS IN CHILDREN

어린이 치면세균막에서 치주질환원인균과 항생제 내성유전자의 출현율

  • Kim, Seon-Mi (Department of Pediatric Dentistry and Dental Research Institute, School of Dentistry, Chonnam National University) ;
  • Choi, Nam-Ki (Department of Pediatric Dentistry and Dental Research Institute, School of Dentistry, Chonnam National University) ;
  • Cho, Seong-Hoon (Department of Pediatric Dentistry and Dental Research Institute, School of Dentistry, Chonnam National University) ;
  • Lee, Seok-Woo (Department of Periodontics and Dental education, School of Dentistry, Chonnam National University) ;
  • Lim, Hoi-Jeong (Department of Orthodontics, School of Dentistry, Chonnam National University) ;
  • Lim, Hoi-Soon (Department of Dental education, School of Dentistry, Chonnam National University) ;
  • Kang, Mi-Sun (Department of Microbiology, College of Medicine, Chonnam National University) ;
  • Oh, Jong-Suk (Department of Microbiology, College of Medicine, Chonnam National University)
  • 김선미 (전남대학교 치의학전문대학원 소아치과학교실 및 치의학연구소) ;
  • 최남기 (전남대학교 치의학전문대학원 소아치과학교실 및 치의학연구소) ;
  • 조성훈 (전남대학교 치의학전문대학원 소아치과학교실 및 치의학연구소) ;
  • 이석우 (전남대학교 치의학전문대학원 치의학교육학교실 및 치주학교실) ;
  • 임회정 (전남대학교 치의학전문대학원 교정학교실) ;
  • 임회순 (전남대학교 치의학전문대학원 치의학교육학교실) ;
  • 강미선 (전남대학교 의과대학 미생물학교실) ;
  • 오종석 (전남대학교 의과대학 미생물학교실)
  • Received : 2011.04.13
  • Accepted : 2011.05.10
  • Published : 2011.05.31
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Abstract

The purpose of this study was to assess the prevalence of periodontopathic bacteria and resistance determinants from oral biofilm of children. Subgingival dental plaque was isolated from 87 healthy children, and PCR was performed to determine the presence of 5 periodontal pathogens including P. gingivalis, T. forsythia, T. denticola, F. nucleatum, A. actinomycetemcomitans, and nine resistance genes including tet(Q), tet(M), ermF, aacA-aphD, cfxA, $bla_{SHV}$, $bla_{TEM}$, vanA, mecA. 1. The prevalence of F. nucleatum, T. forsythia. and P. gingivalis was 95.4%, 55.2%, and 40.2%, respectively. In addition. the prevalence of A. actinomycetemc omitans was 5.7%, while T. denticola was 3.4%. 2. In analysis of antibiotic resistance determinants. cfxA, $bla_{TEM}$ and tet(M) were detected in all the samples tested. It was also found that the prevalence of tet(Q) showing tetracycline resistance. $bla_{SHV}$ associated with resistance to ${\beta}$-lactams, ermF exhibiting erythromycin resistance, and, vanA resulting vancomycin resistance was 88.5%, 29.9% 87.4%, and 48.5%, respectively. The aacA-aphD gene showing resistance to aminoglycosides and mecA gene harboring methicillin resistance exhibited the lowest prevalence with 9.2%. 3. In a correlation analysis between periodontopathic pathogens and antibiotic resistance determinants, it was found that there was a significant correlation between T. forsythia and $bla_{SHV}$. Also, P. gingivalis and vanA showed a correlation. Finally, tet(Q) and ermF showed a significant correlation (phi: 0.514) while mecA and vanA also showed a correlation(phi: 0.25).

항생제 사용에 따라 구강내 세균들이 가지게 되는 내성의 발현이 문제가 될 수 있다. 어린이 치면세균막에서 치주질환의 원인균들과 흔히 사용하고 있는 항생제들에 대한 내성유전자의 출현율을 알아보고자 중합효소연쇄반응을 이용하여 조사하였다. 1. 치주질환 원인균의 출현율은 F. nucleatum 95.4%, T. forsythia 55.2% 이었으며, P. gingivalis 40.2%, A. actinomycetemcomitans 5.7%, T. denticola는 3.4% 순이었다. 2. 항생제 내성유전자의 출현율 조사에서 cephalosporin 분해효소인 cfxA는 100%에서 발견되었으며 ${\beta}$-lactam 분해효소인 $bla_{TEM}$과 tetracycline 내성유전자인 tet(M)도 100%의 출현율을 보였다. tet(Q)는 88.5%, ${\beta}$-lactam 분해효소인 $bla_{SHV}$는 29.9%, macrolide계 내성 ermF유전자는 87.4%, vancomycin 내성 vanA는 48.5%의 출현율을 보였다. Aminoglycoside에 대한 복합 내성을 보이는 aacA-aphD와 meticillin 내성유전자 mecA는 9.2%로 가장 낮은 출현율을 보였다. 3. 치주질환 원인균과 항생제 내성유전자와의 관련성 조사에서 T. forsythia와 $bla_{SHV}$간에 그리고 P. gingivalis와 vanA간 에 유의한 상관성이 있었다. 항생제 내성유전자 tet(Q)와 ermF (0.514)간에 중등도의 상관성을 나타내었으며, mecA와 vanA (0.25)간에 유의한 상관성을 나타내었다. 건강한 어린이들의 치면세균막에 다양한 치주질환 원인균들과 항생제 내성유전자들이 존재하며, 상호 관련성을 가지고 존재함을 보여주었다.

Keywords

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