Journal of the Society of Cosmetic Scientists of Korea (대한화장품학회지)

Society of Cosmetic Scientists of Korea (대한화장품학회)
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Whitening and Antioxidative Effect of Rice Bran Fermented by Bacillus subtilis

발효된 미강의 항산화 효과 및 미백 효과

  • Chae, Ga-Yeon (Department of Biotechnology, College of Medical Life Science, Silla University) ;
  • Kwon, Ryun-Hee (Department of Biotechnology, College of Medical Life Science, Silla University) ;
  • Jang, Min-Woo (Department of Biotechnology, College of Medical Life Science, Silla University) ;
  • Kim, Min-Jung (Department of Biotechnology, College of Medical Life Science, Silla University) ;
  • Ha, Bae-Jin (Department of Biotechnology, College of Medical Life Science, Silla University)
  • 채가연 (신라대학교 일반대학원 생명공학과) ;
  • 권륜희 (신라대학교 일반대학원 생명공학과) ;
  • 장민우 (신라대학교 일반대학원 생명공학과) ;
  • 김민정 (신라대학교 일반대학원 생명공학과) ;
  • 하배진 (신라대학교 일반대학원 생명공학과)
  • Received : 2011.03.15
  • Accepted : 2011.06.17
  • Published : 2011.06.30
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Abstract

In this study, we investigated rice bran fermented by Bacillus subtilis (RFB) at $40^{\circ}C$ for 36 h to develop a new natural antioxidant and whitening agent for new natural cosmetics. RFB showed reactive oxygen species (ROS) scavenging activities in 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, superoxide radical scavenging activities and hydroxyl radical scavenging activities at a concentration of $100 \;{\mu}L$/mL. DPPH, superoxide radical scavenging activities and hydroxyl radical scavenging activities were higher in the RFB than in the RB. Inhibitory activity on auto-oxidation of linoleic acid is also highest at a concentration of $100 \;{\mu}L$/mL. RFB showed the higher inhibitory activity than RB in auto-oxidation. RFB reduced intracellular tyrosinase activity about 80 % at a concentration of $100 \;{\mu}L$/mL. Therefore, we suggest that RFB could be used as a useful antioxidant and whitening agent.

이 연구에서는 천연 항산화소재와 미백소재 개발을 위하여 미강을 Bacillus subtilis에 접종하여 $40^{\circ}C$에서 36 h 동안 발효한 미강추출(RFB)과 80 % ethanol을 이용한 미강추출물(RB)을 사용하여 연구하였다. 추출물의 free radical 소거활성, superoxide radical 소거활성, hydroxyl radical 소거활성은 농도 $100 \;{\mu}L$/mL에서 가장 큰 활성을 나타내었고 리놀산을 이용한 지질과산화 억제 활성 또한 농도 $100 \;{\mu}L$/mL에서 가장 큰 활성을 나타내었고, RB에 비하여 RFB의 더 높은 항산화 능을 확인하였다. Mushroom tyrosinase의 활성 저해효과는 $100 \;{\mu}L$/mL 농도에서 mushroom tyrosinase 의 활성을 80 % 저해하였으며 이는 vitamin C에 비해 더 높은 활성을 나타내었다. 그러므로 우리는 미강 발효추출물이 우수한 항산화와 미백 효능을 갖는 화장품 소재로서 개발 가능성이 클 것으로 기대된다.

Keywords

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