International Journal of Oral Biology

The Korean Academy of Oral Biology (대한구강생물학회)
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Strain-specific PCR Primers for the Detection of Prevotella intermedia ATCC 49046

  • Kim, Min-Jung (Department of Oral Biochemistry, School of Dentistry, Chosun University) ;
  • Min, Jeong-Bum (Department of Conservative Dentistry, School of Dentistry, Chosun University) ;
  • Lim, Sun-A (Department of Dental Hygiene, Songwon University College) ;
  • Kook, Joong-Ki (Department of Oral Biochemistry, School of Dentistry, Chosun University)
  • Received : 2011.04.01
  • Accepted : 2011.05.20
  • Published : 2011.06.30
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Abstract

The aim of this study was to develop Prevotella intermedia ATCC 49046-specific PCR primers designed based on the nucleotide sequence of a DNA probe Pig28. The strainspecificity of the PCR primers, Pig28-F1/Pig28-R1, was confirmed with 9 strains of P. intermedia and 25 strains (15 species) of Prevotella species. The detection limit of the PCR primers was 2 pg of the purified genomic DNA of P. intermedia ATCC 49046. These PCR primers were found to be useful for identifying P. intermedia ATCC 49046, particularly for determining the authenticity of the strain.

Keywords

References

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