The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Molecular biologic mechanism of obesity by GGEx18

경신강지환(輕身降脂丸)18의 분자생물학적인 비만조절 기전에 관한 연구

  • Lee, Hee-Young (Dept. of Formula Science, College of Oriental Medicine and Research Institute of Oriental Medicine, Dongeui University) ;
  • Yoon, Ki-Hyeon (Dept. of Formula Science, College of Oriental Medicine and Research Institute of Oriental Medicine, Dongeui University) ;
  • Seo, Bu-Il (Department of Oriental Herbology, Daegu Haany University) ;
  • Park, Gyu-Ryeol (Department of Oriental Herbology, Daegu Haany University) ;
  • Yoon, Mi-Chung (Dept. of Life Sciences, Mokwon University) ;
  • Shen, Zhi-Bin (Dept. of Chemistry and Analysis of Traditional Chinese Medicine, College of Chinese Materia Medica, Guangdong Pharmaceutical University) ;
  • Cui, Hong-Hua (Dept. of Chemistry and Analysis of Traditional Chinese Medicine, College of Chinese Materia Medica, Guangdong Pharmaceutical University) ;
  • Shin, Soon-Shik (Dept. of Formula Science, College of Oriental Medicine and Research Institute of Oriental Medicine, Dongeui University)
  • 이희영 (동의대학교 한의과대학 및 한의학연구소 방제학교실) ;
  • 윤기현 (동의대학교 한의과대학 및 한의학연구소 방제학교실) ;
  • 서부일 (대구한의대학교 한의과대학 본초학교실) ;
  • 박규열 (대구한의대학교 한의과대학 본초학교실) ;
  • 윤미정 (목원대학교 바이오건강학부) ;
  • 심지빈 (중국 광동약학원 중약학원 중약화학여분석계) ;
  • 최홍화 (중국 광동약학원 중약학원 중약화학여분석계) ;
  • 신순식 (동의대학교 한의과대학 및 한의학연구소 방제학교실)
  • Received : 2011.02.01
  • Accepted : 2011.03.10
  • Published : 2011.03.30
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Abstract

Objectives : This study was undertaken to verify the modulation mechanism of Gyeongshingangjeehwan18 (GGEx18) in ob/ob male mice. Methods : Eight-week old mice (wild-type C57BL/6J and ob/ob) were used for all experiments. Wild-type C57BL/6J mice were used as lean control and obese ob/ob mice were randomly divided into 5 groups : obese control, GGEx15 (Ephedra sinica Stapf + Rheum palmatum L.), GGEx16 (Ephedra sinica Stapf + Laminaria japonica Aresch), GGEx17 (Rheum palmatum L. + Laminaria japonica Aresch), and GGEx18 (Ephedra sinica Stapf + Laminaria japonica Aresch + Rheum palmatum L.). After mice were treated with several kinds of GGEx for 11 weeks, the mRNA expression of peroxisome proliferator-activated receptor (PPAR) target genes and uncoupling protein (UCP) were measured. In addition, $PPAR{\alpha}$ and $PPAR{\beta}$ transactivation was examined in NMu2Li hepatocytes, C2C12 myocytes, and 3T3-L1 preadipocytes using transient transfection assays. Results : 1. Hepatic $PPAR{\alpha}$ target genes, such as ACOX and VLCAD mRNA levels were significantly increased by GGEx18 compared with obese controls. In skeletal muscle, LCAD mRNA expression was stimulated by GGEx16, GGEx17, and GGEx18, whereas MCAD mRNA expression by GGEx17 and GGEx18. $PPAR{\beta}$ target LPL mRNA levels were also increased by GGEx16, GGEx17, and GGEx18 in skeletal muscle, but adipose LPL mRNA levels were decreased. In addition, GGEx18 upregulated UCP mRNA expression in skeletal muslce. 2. $PPAR{\alpha}$ reporter gene expression was increased by GGEx18 in NMu2Li cells compared with vehicle. $PPAR{\alpha}$ and $PPAR{\beta}$ reporter activities were also increased by all GGEx treatments in C2C12 and 3T3-L1 cells. Conclusions : These results suggest that GGEx can act as $PPAR{\alpha}$ and $PPAR{\beta}$ activators, and that GGEx may regulate obesity by stimulating $PPAR{\alpha}$, $PPAR{\beta}$, and UCP activity. Of the 4 compositions, GGEx18 seems to be most effective in improving obesity and lipid disorders.

Keywords

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