Food Science of Animal Resources (한국축산식품학회지)

Korean Society for Food Science of Animal Resources (한국축산식품학회)
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The Analysis for Trans Fatty Acids in Dairy Products Imported to Republic of Korea

수입 유가공품 중 트랜스 지방산 함량 분석

  • Park, Jae-Woo (Livestock Products Standard Division, National Veterinary Research & Quarantine Service) ;
  • Park, Ji-Sung (Livestock Products Standard Division, National Veterinary Research & Quarantine Service) ;
  • Jung, Doo-Kyung (Livestock Products Standard Division, National Veterinary Research & Quarantine Service) ;
  • Song, Sung-Ok (Livestock Products Standard Division, National Veterinary Research & Quarantine Service) ;
  • Woon, Jae-Ho (Seoul Regional Office, National Veterinary Research & Quarantine Service) ;
  • Kim, Jin-Man (Department of Food Science and Biotechnology of Animal Resources, Konkuk University) ;
  • Wee, Sung-Hwan (Livestock Products Standard Division, National Veterinary Research & Quarantine Service)
  • 박재우 (국립수의과학검역원 축산물규격과) ;
  • 박지성 (국립수의과학검역원 축산물규격과) ;
  • 정두경 (국립수의과학검역원 축산물규격과) ;
  • 송성옥 (국립수의과학검역원 축산물규격과) ;
  • 운재호 (국립수의과학검역원 서울지원) ;
  • 김진만 (건국대학교 축산식품생물공학과) ;
  • 위성환 (국립수의과학검역원 축산물규격과)
  • Received : 2011.03.30
  • Accepted : 2011.06.13
  • Published : 2011.06.30
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Abstract

In this study, the most preferred trans fatty acid analysis methods, AOAC 996.06 and the Korea Food and Drug Administration official method, were reviewed and modified to apply to dairy products and dairy products imported into the Republic of Korea for evaluating trans fatty acid (TFA) content. The Rose-Gottlieb method for total fat analysis was validated with accuracy and precision parameters by analyzing infant formula standard reference material provided by the National Institute for Standards and Technology. The accuracy and precision data satisfied the CODEX guidelines. TFAs were analyzed with a resolution of 1.5 for 45 min using the modified oven temperature program. This modified method was applied to 45 dairy products from 11 countries. Average TFA contents in these imported dairy products ranged from 0.1 to 5.4 g per 100 g product. The majority of dairy products imported into the Republic of Korea were cheeses. TFA contents in the cheeses were 0.1 to 2.4 g per 100 g cheese. TFA contents in other dairy products were 1.7 to 5.4 g per 100 g product. These TFAs content variations can be explained by the trans fatty acids naturally present in ruminant milk formed by bacterial bio-hydrogenation in the rumen of cows and the different vegetable fat used as ingredients in the final products.

본 연구는 유가공품에 적용할 수 있는 트랜스 지방산 분석조건을 확립하고 우리나라로 수입되는 유제품의 트랜스 지방산 함량 수준을 확인하기 위해 실시되었다. 조지방 추출은 Rose-Gottlieb법으로 조제분유 표준인증물질을 분석하고 그 인증값과 비교하였던 바, CODEX 가이드에 부합되는 정확도를 나타냈다. 유제품의 트랜스 지방산 분석을 위해 가스크로마토그래프 오븐 온도 상승 조건을 다양하게 변형하여, 트랜스 지방산들이 양호한 분해능을 보이면서 분석되는 적합한 온도 조건을 제시하였고, 최적화된 분석법을 사용하여 우리나라로 수입되는 유가공품(13개 유형, 45제품)에 적용하였다. 수입되는 유제품의 트랜스 지방산 함량은 제품 100 g 당 0.1-5.4 g 범위를 나타내었다. 가장 많이 수입이 되는 치즈류는 0.1-2.4 g/100 g 제품(평균값 1.1 g/100 g, n=35)으로 확인된 반면, 치즈를 제외한 유가공품은 1.7-5.4 g/100 g 제품 수준이었다. 이러한 트랜스 지방산 함량은 반추동물의 제1위 미생물에 의해서 생성되어 우유 중에 존재하는 트랜스 지방산 함량수준이며, 원료로 사용되는 우유의 트랜스 지방산 함량이나 부가적으로 첨가되는 식물성 지방에서 유래되는 트랜스 지방산의 함량에 따라 검사대상 유제품에서 함량의 차이는 편차가 있을 것으로 평가하였다

Keywords

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