The Journal of Korean Obstetrics and Gynecology (대한한방부인과학회지)

The Society of Korean Medicine Obstetrics and Gynecology (대한한방부인과학회)
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Inhibitory Effects of Wogonin Mixed with Hydrogel on Osteoclast Differentiation

우고닌-하이드로젤 지지체의 파골세포 분화 억제 효과

  • Yang, Na-Rae (Dept. of Gynecology, College of Oriental Medicine, Kyung-Hee University) ;
  • Lee, Jin-Moo (Dept. of Gynecology, College of Oriental Medicine, Kyung-Hee University) ;
  • Lee, Chang-Hoon (Dept. of Gynecology, College of Oriental Medicine, Kyung-Hee University) ;
  • Jang, Jun-Bock (Dept. of Gynecology, College of Oriental Medicine, Kyung-Hee University) ;
  • Lee, Kyung-Sub (Dept. of Gynecology, College of Oriental Medicine, Kyung-Hee University)
  • 양나래 (경희대학교 한의과대학 한방부인과) ;
  • 이진무 (경희대학교 한의과대학 한방부인과) ;
  • 이창훈 (경희대학교 한의과대학 한방부인과) ;
  • 장준복 (경희대학교 한의과대학 한방부인과) ;
  • 이경섭 (경희대학교 한의과대학 한방부인과)
  • Received : 2011.10.31
  • Accepted : 2011.11.07
  • Published : 2011.11.25
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Abstract

Purpose: Wogonin is an active component isolated from scutellariae radix. This study was conducted to evaluate the inhibitory effect of wogonin mixed with hydrogel on osteoclast differentiation. Methods: It was performed to estimate cytotoxicity of Wogonin alginate hydrogel disk(WHD) in BMMs stimulated with RANKL, M-CSF. ROS synthesis and actin ring formation were analysed to observe the effect of WHD. Results: WHD has no cytotoxicity at the concentration of 0.1 ${\mu}g/ml$ or lower. 0.1 ${\mu}g/ml$, 1 ${\mu}g/ml$ WHD restrained the synthesis of ROS and 0.1 ${\mu}g/ml$, 1 ${\mu}g/ml$ WHD restrained the formation of actin ring. Conclusions: WHD has the inhibitory effect of osteoclast differentiation and bone resorption. Further studies are needed to treat osteoporosis by herbal medicine.

Keywords

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