BMB Reports

Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Suppression of TNF-alpha-induced MMP-9 expression by a cell-permeable superoxide dismutase in keratinocytes

  • Song, Ha-Yong (Department of Biomedical Science, Research Institute for Bioscience & Biotechnology, Hallym University) ;
  • Ju, Sung-Mi (Department of Biomedical Science, Research Institute for Bioscience & Biotechnology, Hallym University) ;
  • Goh, Ah-Ra (Department of Biomedical Science, Research Institute for Bioscience & Biotechnology, Hallym University) ;
  • Kwon, Dong-Joo (Department of Biomedical Science, Research Institute for Bioscience & Biotechnology, Hallym University) ;
  • Choi, Soo-Young (Department of Biomedical Science, Research Institute for Bioscience & Biotechnology, Hallym University) ;
  • Park, Jin-Seu (Department of Biomedical Science, Research Institute for Bioscience & Biotechnology, Hallym University)
  • Received : 2011.05.02
  • Accepted : 2011.05.06
  • Published : 2011.07.31
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Abstract

Up-regulation of selected matrix metalloproteinases (MMPs) such as MMP-9 contributes to inflammatory processes during the development of various skin diseases, such as atopic dermatitis. In this study, we examined the effect of a cell-permeable superoxide dismutase (Tat-SOD) on TNF-${\alpha}$-induced MMP-9 expression in human keratinocyte cells (HaCaT). When Tat-SOD was added to the culture medium of HaCaT cells, it rapidly entered the cells in dose- and time-dependent manners. Tat-SOD decreased TNF-${\alpha}$-induced reactive oxygen species (ROS) generation. Tat-SOD also inhibited TNF-${\alpha}$-induced NF-${\kappa}B$ DNA binding activity. Treatment of HaCaT cells with Tat-SOD significantly inhibited TNF-${\alpha}$-induced mRNA and protein expression of MMP-9, as measured by RT-PCR and Western blot analysis. In addition, Tat-SOD suppressed TNF-${\alpha}$-induced gelatinolytic activity of MMP-9. Taken together, our results indicate that Tat-SOD can suppress TNF-${\alpha}$-induced MMP-9 expression via ROS-NF-${\kappa}B$-dependent mechanisms in keratinocytes, and therefore can be used as an immunomodulatory agent against inflammatory skin diseases related to oxidative stress.

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References

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