The Korean Journal of Food And Nutrition (한국식품영양학회지)

The Korean Society of Food and Nutrition (한국식품영양학회)
권호 표지
DOI QR코드

DOI QR Code

Isolation of Berberine from the Rhizome of $Coptis$ $chinensis$ by Centrifugal Partition Chromatography

향류분배 크로마토그라피법에 의한 황련($Coptis$ $chinensis$) 뿌리로 부터 Berberine의 분리

  • Kim, Jung-Bae (Dept. of Food Nutrition and Cookery, Sangji Youngseo College)
  • 김중배 (상지영서대학 식품영양조리과)
  • Received : 2011.10.12
  • Accepted : 2011.12.20
  • Published : 2011.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

$Coptis$ $chinensis$ Franch rhizome is one of the important traditional Korea medicines with anti-inflammatory, anti-bacterial, anti-hypertensive and anti-cancer properties. The methanol extract of rhizome from the $Coptis$ $chinensis$ rhizome was purified by using preparative centrifugal partition chromatography (CPC) and HPLC method. The optimum two-phase CPC solvent system was composed of n-butanol:acetic acid:water at a ratio of 4:1:5. Berberine (16.8 mg) was successfully isolated by CPC and HPLC. The chemical structure of the compound was identified by (1 H)$^1H$, $^{13}C$-NMR and ESI-MS spectral data analysis.

황련은 염증, 항균, 고혈압 및 항암 작용을 지닌 중요한 전통 한약재이다. 황련 뿌리 메탄올 추출성분은 CPC와 HPLC 방법으로 정제하였다. CPC의 최적 용매 조건은 n-butanol:acetic acid:water(4:1:5)이었다. Berberine(16.8 mg)은 CPC와 HPLC 방법에 의하여 효과적으로 분리하였다. 이 물질의 화학적 구조는 $^1H$, $^{13}C$-NMR과 ESI-MS 데이터 분석에 의하여 확인되었다.

Keywords

References

  1. Chen J, Wang F, Liu J, Lee FSC, Wang X, Yang H. 2008. Analysis of alkaloids in Coptis chinesis Franch by accelerated solvent extraction combined with ultra performance liquid chromatographic analysis with photodiode array and tandem mass spectrometry detections. Analytica Chimica Acta 613:184-195 https://doi.org/10.1016/j.aca.2008.02.060
  2. Cheong JH, Kim CY, Kim JW. 2007. Preparative isolation and purification of sinomenine from Sinomenium acutum by centrifugal partition chromatography. J Sep Sci 30:2105-2108 https://doi.org/10.1002/jssc.200700019
  3. Deevanhxay P, Suzuki M, Maeshibu N, Li H, Tanaka K, Hirose S. 2009. Simultaneous characterization of quaternary alkaloids, 8-oxoprotoberberine alkaloids, and a steroid compound in Coscinium fenistratum by liquid chromatography hybrid ion time-of-flight mass spectrometry. J Pharm Biomed Anal 50: 413-425 https://doi.org/10.1016/j.jpba.2009.05.023
  4. Kim CY, Ahn MJ, Kim JW. 2006. Preparative isolation of mangiferin from Anemarrhena asphodeloides rhizomes by centrifugal partition chromatography. J Sep Sci 29:869-875
  5. Kim CY, Kim JW. 2007. Preparative isolation and purification of geniposide from gardenia fruits by centrifugal partition chromatography. Phytochemical Analysis 18:115-117 https://doi.org/10.1002/pca.958
  6. Kim SM, Sang YF, Um BH. 2010. Preparative separation of chlorogenic acid by centrifugal partition chromatography from highbush blueberry leaves. Phytochemical Analysis 21: 457-462 https://doi.org/10.1002/pca.1218
  7. Li CY, Tsai SI, Damu AG, Wu TS. 2009. A rapid and simple determination of protoberberine alkaloids in rhizoma Coptidis by H NMR and its application for quality control of commercial prescriptions. J Pharm Biomed Anal 49:1272-1276 https://doi.org/10.1016/j.jpba.2009.02.028
  8. Min BS, Cho JS. 2008. Quantitive determination of protobeberine from the roots of Coptis chinensis. Natural Product Scinces 14:68-72
  9. Osamu S, Kumi N, Setsuko S, Noriko K, Masanori K, Makoto I, Sanae O, Mitsuyasu U. 2008. Preparative separation of the saponin lancemaside A from Codonopsis lanceolate by centrifugal partition chromatography. Phytochemical Analysis 19:403-410 https://doi.org/10.1002/pca.1065
  10. Poucault AP. 1994. Centrifugal Partition Chromatography. pp 51-239. Marcel Dekker
  11. Seneviratne CJ, Wong RWK, Samaranayake LP. 2007. Potent anti-microbial activity of traditional Chinese medicine herbs against Candida species. Mycoses 51,30-34
  12. Yin L, Lu B, Lu Y, Xu L, Han X, Xu Y, Peng J, Sun C. 2009. Simultaneous determination of 11 active components in two well-known traditional Chinese medicines by HPLC coupled with diode array detection for quality control. J Pharm Biomed Anal 49:1101-1108 https://doi.org/10.1016/j.jpba.2009.01.039

Cited by

  1. Isolation and Purification of Berberine in Cortex Phellodendri by Centrifugal Partition Chromatography vol.27, pp.3, 2014, https://doi.org/10.9799/ksfan.2014.27.3.532
  2. Analysis of Volatile Compounds in Phellodendron amurense Ruprecht, Coptis japonica Makino, and Chelidonium majus var. asiaticum by TD GC/MS vol.50, pp.5, 2013, https://doi.org/10.12772/TSE.2013.50.275
  3. Isolation and Purification of Antibacterial Components in Cortex Phellodendri vol.26, pp.3, 2013, https://doi.org/10.9799/ksfan.2013.26.3.547