The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Inhibitory Effect of Paraconiothyrium minitans CM2 on Sclerotial Germination of Sclerotinia sclerotiorum and S. minor Causing Sclerotinia Rot of Lettuce

Paraconiothyrium minitans CM2의 상추균핵병균(Sclerotinia sclerotiorum, S. minor) 균핵 발아에 대한 억제 효과

  • Lee, Sang-Yeob (Agricultural Microbiology Team, National Academy of Agricultural Science (NAAS), Rural Development Administration (RDA)) ;
  • Kim, Wan-Gyu (Agricultural Microbiology Team, National Academy of Agricultural Science (NAAS), Rural Development Administration (RDA)) ;
  • Hong, Sung-Kee (Crop Protection Division, NAAS, RDA) ;
  • Weon, Hang-Yeon (Agricultural Microbiology Team, National Academy of Agricultural Science (NAAS), Rural Development Administration (RDA)) ;
  • Park, Kyung-Seok (Agricultural Microbiology Team, National Academy of Agricultural Science (NAAS), Rural Development Administration (RDA))
  • 이상엽 (농촌진흥청 국립농업과학원 농업미생물팀) ;
  • 김완규 (농촌진흥청 국립농업과학원 농업미생물팀) ;
  • 홍성기 (농촌진흥청 국립농업과학원 작물보호과) ;
  • 원항연 (농촌진흥청 국립농업과학원 농업미생물팀) ;
  • 박경석 (농촌진흥청 국립농업과학원 농업미생물팀)
  • Received : 2011.07.20
  • Accepted : 2011.07.25
  • Published : 2011.08.01
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Abstract

One fungal isolate CM2 parasitic to Sclerotinia sclerotiorum and S. minor causing Sclerotinia rot of lettuce was identified as Paraconiothyrium minitans based on its morphological and molecular characteristics. P. minitans CM2 grew best on PDA with pH 6.5 at $22^{\circ}C$ under alternating cycles of 12 hr near ultraviolet light and 12 hr darkness. Scleroria of S. sclerotiorum and S. minor treated with conidial suspension of P. minitans CM2 did not directly germinate and produced no apothecia.

2종의 상추균핵병균(S. sclerotiorum, S. minor)에 기생성인 선발진균(CM2)의 형태적 및 분자적 특성을 조사한 결과, Paraconiothyrium minitans로 동정되었다. P. minitans CM2의 균사 배양조건은 pH 6.5로 조절한 PDA배지를 사용하고, $22^{\circ}C$에서 1일 12시간 주기의 근자외선광을 처리하여 배양하는 것이 가장 좋은 것으로 나타났다. P. minitans CM2의 분생포자현탁액을 상추균핵병균의 균핵에 처리하여 발아에 미치는 영향을 조사한 결과, 균핵의 직접발아와 자낭반 형성이 전혀 이루어지지 않았다.

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