Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Isolation of a Nonylphenol-degrading Microbial Consortium

Nonylphenol 분해 미생물 컨소시엄 균주 개발

  • Song, Won (Department of Food Science and Technology, Pukyong National University) ;
  • Lim, Keun-Sick (Department of Food Science and Technology, Pukyong National University) ;
  • Yu, Dae-Ung (Department of Food Science and Technology, Pukyong National University) ;
  • Park, Mi-Eun (Department of Ecological Engineering, Pukyong National University) ;
  • Jeong, Eun-Tak (SNTECH Co., Ltd.) ;
  • Kim, Dong-Myung (Department of Ecological Engineering, Pukyong National University) ;
  • Chung, Yong-Hyun (Department of Ecological Engineering, Pukyong National University) ;
  • Kim, Young-Mog (Department of Food Science and Technology, Pukyong National University)
  • Received : 2011.04.15
  • Accepted : 2011.07.02
  • Published : 2011.08.30
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Abstract

Nonylphenol (NP), which is well known as an endocrine disrupter, has been detected widely in untreated sewage or waste water streams. Given the necessity of discovering an eco-friendly method of degrading this toxic organic compound, this study was conducted to isolate NP-degrading microorganisms from the aqueous environment. NP-degrading microbes were isolated through NP-containing enrichment culture. Finally, a microbial consortium, SW-3, capable of degrading NP with high efficiency, was selected from the mixture sample. The microbial consortium SW-3 was able to degrade over 99% of 100 ppm NP in the culture medium for 40 days at $25^{\circ}C$. The microbial consortium SW-3 seemed to utilize NP as a carbon source, since NP was the sole carbon source in the culture medium. In order to isolate the NP-degrading bacterium, we further conducted single colony isolation using the microbial consortium SW-3. Four strains isolated from SW-3 exhibited lower NP-degradation efficiency than that of SW-3, suggesting that NP was degraded by the co-metabolism of the microbial consortium. We suggest that the microbial consortium obtained in this study would be useful in developing an eco-friendly bioremediation technology for NP degradation.

Keywords

References

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