The Korean Journal of Food And Nutrition (한국식품영양학회지)

The Korean Society of Food and Nutrition (한국식품영양학회)
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Anticancer Activity and Quantitative Analysis of Glucosinolates from Green and Red Leaf Mustard

청갓과 적갓에 함유된 Glucosinolates의 항암 활성 및 정량 분석

  • 김활 (경기북과학고등학교) ;
  • 김준열 (경기북과학고등학교) ;
  • 김효진 (경기북과학고등학교) ;
  • 김도경 (경기북과학고등학교) ;
  • 조혜진 (경기북과학고등학교) ;
  • 한병수 (경기북과학고등학교) ;
  • 김헌웅 (국립농업과학원 농식품자원부) ;
  • 김정봉 (국립농업과학원 농식품자원부)
  • Received : 2011.07.07
  • Accepted : 2011.07.27
  • Published : 2011.09.30
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Abstract

The objective of this study was to compare the anticancer activity of glucosinolates against four different cancer cells; SNU-251, SNU-354, SNU-C4, MCF-7 and to determine the amounts of glucosinolates in mustard leaves. Green and red mustard leaves were cultivated on the field of Rural Development Administration from May to July, 2010. After the leaves were harvest and lypophilized, the fine powder was extracted with boiling 70% methanol(v/v) for the measurement of anticancer activity and then applied to the cancer cells obtained from Korean Cell Line Bank(KCLB). The anticancer activity of red leaf mustard was higher than that of green mustard leaf, particularly in SNU-251 for 24 hr and in SNU-C4 for 48 hr. In both green and red mustard leaf, sinigrin was the most abundant glucosinolte and was determined as 4.71 and 3.06 mg/g dry wt., respectively, but glucoiberin only presented 0.14 mg/g dry wt. with minor amounts.

청갓과 적갓에서 추출한 GSLs에 의한 4종류의 암세포주를 이용하여 항암 활성을 조사하였다. 또한 갓 추출물에 함유된 GSLs를 HPLC와 LC-ESI/MS로 분석한 결과는 다음과 같다. 1. 갓에서 추출한 GSLs의 항암 활성을 분석한 결과, SNU-354 (간암)>SNU-C4결정암)>SNU-251(난소암)>MCF-7(유방암) 순으로 높게 나타났다. 또한 GSLs 추출물의 처리 시간(24, 48시간)에 따르면 적갓 추출물의 항암 효과가 청갓 추출물의 항암 효과보다 큰 것으로 나타났다. 2. 갓의 주요한 GSLs 성분은 sinigrin이었으며, 청갓이 적갓보다 더 높은 함량을 나타내었으며, 또한 청갓에서만 glucoiberin이 미량으로 검출되었다. 3. 항암 활성 평가에서 적갓 GSLs 추출물이 청갓보다 더 높은 활성을 나타낸 것은 GSLs 물질 이외에 다른 물질들이 관여 했을 것으로 생각되므로 이에 대한 추가적인 연구가 필요할 것으로 사료된다.

Keywords

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