Ocean and Polar Research

Korea Institute of Ocean Science & Technology (한국해양과학기술원)
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Antioxidant Activity of Flavonoids Isolated from Vitex rotundifolia

순비기나무(Vitex rotundifolia)로부터 분리한 플라보노이드 성분의 항산화 활성

  • Kim, You-Ah (Natural Compounds Bank Establishment Department, DGOM) ;
  • Lee, Jung-Im (Division of Marine Environment & Bioscience, College of Ocean Science and Technology, Korea Maritime University) ;
  • Hong, Joo-Wan (Division of Marine Environment & Bioscience, College of Ocean Science and Technology, Korea Maritime University) ;
  • Jung, Myoung-Eun (Division of Marine Environment & Bioscience, College of Ocean Science and Technology, Korea Maritime University) ;
  • Seo, Young-Wan (Division of Marine Environment & Bioscience, College of Ocean Science and Technology, Korea Maritime University)
  • 김유아 ((재)대구경북한방산업진흥원 천연물물질은행구축사업단) ;
  • 이정임 (한국해양대학교 해양과학기술대학 해양환경.생명과학부) ;
  • 홍주완 (한국해양대학교 해양과학기술대학 해양환경.생명과학부) ;
  • 정명은 (한국해양대학교 해양과학기술대학 해양환경.생명과학부) ;
  • 서영완 (한국해양대학교 해양과학기술대학 해양환경.생명과학부)
  • Received : 2011.08.17
  • Accepted : 2011.09.07
  • Published : 2011.09.30
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Abstract

The aim of this investigation was to evaluate antioxidant activity of crude extracts from the halophyte Vitex rotundifolia, their solvent fractions, and isolated compounds (1-3). Antioxidant capacity was determined by measuring DPPH radical, and authentic $ONOO^-$ and $ONOO^-$ generated from 3- morpholinsydnonimine (SIN-1) in vitro as well as degree of occurrence of intracellular ROS, NO and GSH in mouse macrophage Raw 264.7 cells. From comparative analysis, MeOH extract, n-BuOH, and 85% aq. MeOH solvent fractions showed significant antioxidant effect in DPPH radical and $ONOO^-$ assay systems. Activity-guided purification of n-BuOH and 85% aq. MeOH fractions led to the isolation of flavonoids 1-3. Among them, compound 1 exhibited excellent antioxidant effect in all bioassay systems tested. On the other hand, compounds 2 and 3 revealed potent inhibitory effect against $ONOO^-$ generated from SIN-1, comparable with the positive control penicillamine.

Keywords

References

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  1. Isolation of a New Labdane-type Diterpene from Vitex rotundifolia vol.34, pp.12, 2013, https://doi.org/10.5012/bkcs.2013.34.12.3840
  2. Chemical Constituents of the Halophyte Vitex rotundifolia vol.58, pp.6, 2014, https://doi.org/10.5012/jkcs.2014.58.6.686