Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
권호 표지
DOI QR코드

DOI QR Code

Effects of Ixeris dentata extract on the production of pro-inflammatory cytokines in the LPS stimulated rat and Raw 264.7 cells

씀바귀 추출물이 LPS투여 흰쥐 및 Raw 264.7세포에서 전염증성 cytokines 생성에 미치는 영향

  • Lee, Eun (Department of Pharmaceutical Engineering, College of Health Sciences, Sangji University)
  • 이은 (상지대학교 보건과학대학 제약공학과)
  • Received : 2011.07.13
  • Accepted : 2011.09.20
  • Published : 2011.10.31
Download PDF
⟨ Previous Next ⟩

Abstract

The purpose of this study was to investigate anti-inflammatory effect of Ixeris dentata ethanol extract in lipopolysaccharide-exposed rats and Raw 264.7 cells. Plasma concentrations of IL-$1{\beta}$, IL-6 and TNF-${\alpha}$ were lower in Ixeris dentata-treated groups than in control group. Concentration of plasma IL-10 was higher in Ixeris dentata-treated groups than in control group. Concentrations of liver IL-$1{\beta}$ and IL-6 were lower in the Ixeris dentata-treated groups than in control group. However, concentrations of liver TNF-${\alpha}$ and IL-10 were not significantly different among all treatment groups. In the study using lipopolysaccharide-exposed Raw 264.7 cells, concentrations of IL-$1{\beta}$, IL-6 and TNF-${\alpha}$ tended to be decreased, but concentration of IL-10 tended to be increased in Ixeris dentata-treated groups. Plasm concentrations of total protein and albumin appeared to be increased in Ixeris dentata-treated groups.

씀바귀추출물이 LPS shock 염증반응에서 항염증효과에 미치는 영향을 검토하기 위하여 생체 및 세포배양실험에서 전염증성 cytokine들의 생성과 혈액 내 생물학적 수치를 조사했다. 그 결과 LPS 염증유도 rat에서 씀바귀 추출물은 혈액 내 전염증성 cytokines 들, 즉 IL-$1{\beta}$, IL-6 및 TNF-${\alpha}$의 생산을 저해하고, 한편으로는 IL-10의 생산을 촉진하였다. 간장 IL-$1{\beta}$ 및 IL-6의 농도는 씀바귀추출물 처리군이 대조군보다 유의하게 낮은 값을 나타내었으나, TNF-${\alpha}$ 및 IL-10의 농도는 모든 처리군 간에 유의한 차이를 나타내지 않았다. Raw 264.7 cell의 세포배양실험에서는 TNF-${\alpha}$의 농도가 유의한 차이를 나타내지는 않았지만, 3개 cytokine 모두가 씀바귀 투여량이 증가함에 따라 감소하는 경향을 보였다. IL-10의 농도는 씀바귀 처리군 들 모두가 높은 경향을 나타내었다. 혈액 내 total protein 및 albumin의 농도는 대조군과 유의한 차이를 나타내지는 않았으나, 씀바귀추출물 처리 군에서 높은 경향을 보였다. 이상의 결과들을 종합해 보면 씀바귀 추출물에는 항염증반응에 관여하는 기능성물질이 내재하고 있음을 시사해 준다.

Keywords

References

  1. Abul., K., H. Andrew and S.P. Lichtman. 2007. Cellular and molecular immunology. 6th edition. pp. 353-354.
  2. Arai, Y.Y., M. Kusumoto, K. Nagao and H. Shiojima. 1963. Composite constituents: Aliphatics and triterpenoids isolated from the whole plants of Ixers debilis and I. dentata. Yakugaku Zasshi 103:356-359.
  3. Barton, C.C., E. Barton, P.E. Ganey, S.L. Kunkel and R.A. Roth. 2001. Bacterial lipopolysaccharide enhances aflatoxin B1 hepatotoxicity in rats by a mechanism that depends on tumor necrosis factor-${\alpha}$. Hepatology 33:66-73. https://doi.org/10.1053/jhep.2001.20643
  4. Benvenistem, E.N. 1997. Immunology of the Nervous System. Oxford University Press, New York. USA. pp. 419-459.
  5. Benvenistem E.N. 1998. Cytokine actions in the central nervous system. Cytokine Growth Factor Rev. 9:259-275. https://doi.org/10.1016/S1359-6101(98)00015-X
  6. Bhattacharyya, A.,S. Pathak, S. Datta, S. Chattopadhyay, J. Basu and M. KunDu. 2002. Mitogenactivated protein kinases and NF-kappaB regulate H. pylori-mediated IL-8 release from macrophages. Biochem J. 366:376-82.
  7. Binetruy, B., T. Smeal and M. Kariu. 1991. Ha-Ras augments c-Jun activity and stimulates phosphoylation of its activation domain. Nature 351:122-7. https://doi.org/10.1038/351122a0
  8. Boumpas, D.T., G.P. Chrousos, R.L. Wilder, T.R. Cupps and J.E. Balow. 1993. Glucocorticoid therapy of immune-mediated diseases: basic and clinical correlates. Ann. Intern. Med. 119:1198-1208. https://doi.org/10.7326/0003-4819-119-12-199312150-00007
  9. Briscoe, D.M., R.S. Cotran and J.S. Pober. 1992. Effect of tumor necrosis factor, lipopolysaccharide, and IL-4 on the expression of vascular cell adhesion molecule-1 in vivo: correlation with CD3+T cell infiltration. J. Immunol. 149:2954-60.
  10. Busam, K.J., T.M. Bauer, J. Bauer, W. Gerok and K. Decker. 1990. Interleukin-6 release by rat liver macrophages. J. Hepatol. 11:367-373. https://doi.org/10.1016/0168-8278(90)90223-E
  11. Byun, B.H.. 2005. Effects of Inonotus obliquus Ethanol Extract on Cytokine Expression in Raw 264.7 Cell. Korean J. Herbology 20(2):55-60.
  12. Chamulitrat, W., M.E. Blazka, S.J. Jordan, M.I. Luster and R.P. Mason. 1995. Tumor necrosis factor-${\alpha}$ and nitric oxide production in endotoxin-primed rats administered carbon tetrachloride. Life Sci. 24:2273-80.
  13. Chao, C.Y., S.L. Yeh, M.T. Lin and W.J. Chen. 2000. Effects of parenteral infusion with fish-oil or safflower-oil emulsion on hepatic lipids, plasma amino acids, and inflammatory mediators in septic rats. Nutrition 16:284-288. https://doi.org/10.1016/S0899-9007(99)00299-3
  14. Cho, J.S., H.Y. chung and H.S. Young. 1990. A preliminary study on hypo-cholesterolemic and hypoglycemic activities of some medical plants. Korean J. phamacogn. 21:153-155.
  15. Choi, J.S., H.S. Young and B.W. Kim. 1991. Hypoglycemic and hypolipodemic effects of Ixeris dentata in diabetes rats. Arch. Pham. Res. 13:269-270.
  16. Clerici, M., E. Ferrario, D. Trabattoni, S. Viviani, V. Bonfganti and D.J. Vanzon . 1994. Multiple defects of T helper cell function in newly diagnosed patients with Hodgkin's disease. European J. Cancer 30A:1464-1470.
  17. Funk, C.D., L.B. Frunk, M.E. Kennedy, A.S. Pong and. G.A. Fitzgerald.1991. Human platelet/ erythroleukemia cell prostaglandin G/H synthase: cDNA cloning, expression, and gene chromosomal assignment. FASEB J. 5:2304-2312.
  18. Gamber, J.R., J.M. Harlan, S.J. Klebanoff and M.A. Vadas. 1985. Stimulation of the adherence of neutrophils to umbilical vein endothelium by human recombinant tumor necrosis factor. Proc. Natl. Acad. Sci. USA. 82:8667-8671. https://doi.org/10.1073/pnas.82.24.8667
  19. Hamada, E., T. Nishida, Y. Uchiyama, J. Nakamura, K. Isihara and H. Kazuo. 1999. Activation of Kupffer cells and caspases-3 involved in rat hepatocyte apoptosis induced by endotoxin. J. Hepatol. 30:807-818. https://doi.org/10.1016/S0168-8278(99)80133-0
  20. Harbrecht, B.G., M. Disilvio, A.J. Demetris, R.L. Simmons, and T.R. Billiar. 1994. Tumor necrosis factor-${\alpha}$ regulates in vivo nitric oxide synthesis and induces liver injury during endotoxemia. Hepatology 20:1055-1060. https://doi.org/10.1002/hep.1840200439
  21. Hibi, M., K. Nakajima and T. Hirano. 1996. IL-6 cytokine family and signal transduction: a model of the cytokine system. J. Mol. Med. 4(1):1-12.
  22. Hill, D.B., L.S. Marsano and C.J. McClain. 1993. Increased plasma interleukin-8 concentrations in alcoholic hepatitis. Hepatology 18:576-580. https://doi.org/10.1002/hep.1840180316
  23. Hirano, T., T. Matsuda and K. Nakajima. 1994. Signal transduction through gp130 that is shared among the receptors for the interleukin 6 related cytokine subfamily. Stem Cells 12(3):262-277. https://doi.org/10.1002/stem.5530120303
  24. Huang, Y.S., C.Y. Chan, J.C. Wu, C.H. Pai, Y. Chao and S.D. Lee. 1996. Serum levels of interleukin-8 in alcoholic liver disease: relationship with disease stage, biochemical parameters and survival. J. Hepatol. 24:377-384. https://doi.org/10.1016/S0168-8278(96)80156-5
  25. Hukuta, J.R. 1988. Illustrate Medicinal Plants of the World. Amazon. Co. Tokyo, Japan. pp. 1067-1068.
  26. Jirik, F.R., T.J. Podor and T. Hirano. 1988. Bacterial lipopolysaccharide and inflammatory mediators augment IL-6 secretion by human endothelial cells. J. Immunol. 142:144-147.
  27. Kakuma, S., A. Fukatsu, T. Shinagawa, S. Kurokawa and A. Kusakabe. 1992. Localization of intra hepatic interleukin-6 in patients with acute and chronic liver disease. J. Clin. Pathol. 45:408-411. https://doi.org/10.1136/jcp.45.5.408
  28. Lee, E. 2007. Anti-inflammatory effect of Scutellariae Radix. Korean J. Plant Res. 20(6):548-522.
  29. Louis, H., O. LeMoine, M.O. Peny, E. Quertinmont, D. Fokan and M. Goldman. 1997. Production and role of interleukin-10 in concanavalin A- induced hepatitis in mice. Hepatology 25:1382-1389. https://doi.org/10.1002/hep.510250614
  30. Marriot, J.B., M. Westby, S. Cookson, M. Guckian, S. Goodbourn and G. Muller . 1988. CC-3052: a water-soluble analog of thalidomide and potent inhibitor of activation-induced TNF-${\alpha}$ production. J. Immunol. 161:4236-4243.
  31. Mathiak, G., G. Grass, T. Herzmann and T. Luebke. 2000. Cu-Zetina C, Boehm SA. Capase-1-inhibitor ac-YVAD-cmk reduces LPS-lethality in rats without affecting haematology or cytokine responses. British J. Pharmacol. 131:383-386. https://doi.org/10.1038/sj.bjp.0703629
  32. Murphy, S., M.L. Simmons, L. Agullo, A. Garcia, D.L. Feinstein, E. Galea,D.J. Reis DJ, D. Minc-Golomb and J.P. Schwartz. 1993. Synthesis of nitric oxide in CNS glial cells. Trends Neurosci. 16:323-328. https://doi.org/10.1016/0166-2236(93)90109-Y
  33. Murphy, S. 2000. Production of nitric oxide by glial cells: regulation and potential roles in the CNS. Glia 29:1-13. https://doi.org/10.1002/(SICI)1098-1136(20000101)29:1<1::AID-GLIA1>3.0.CO;2-N
  34. Pender, S.L., E.J. Breese and U. Gunther. 1999. Suppression of T-cell-mediated injury in human gut by interleukin 10: Role of matrix metalloproteinases. Gastroenterology 115:573-583.
  35. Rabson, A., I.M. Roitt and P.J. Delves. 2005. Really Essential Medical Immunology. Blackwell publishing Ltd, Oxford, UK. pp. 1-14.
  36. Schotte, H., B. Schluter, P. Willeke, E. Mickholz, A. Schora, W. Domschke, and M. Gaubitz. 2004. Long-term treatment with etanercept significantly reduces the number of proinflammatory cytokine secteting peripheral blood mononuclear cells in patients with rheumatoid arthritis. Rheumatology 4:112-118.
  37. Seo, W.G., H.O. Pae, G.S. Oh , K.Y. Chai, T.O. Kwon, Y.G. Yun, N.Y. Kim and H.T. Chung. Inhibitory rotundus rhizomes on nitric oxide and superoxide production by murine macrophage cell line, Raw 264.7 cells. J. Ethnopharmacol. 76:59-64.
  38. Seto, M., T. Miyasa and S. Fukushima. 1986. Sesquiterpene lactones from Ixeris dentata Nakai. Chem. Pham. Bull. 34:4170-4176. https://doi.org/10.1248/cpb.34.4170
  39. Simpson, K.J., N.W.Lukacs, L. Colletti, R.M. Strieter and S.L. Kunkel. 1997. Cytokines and the liver. J. Hepatol. 27:1120-32. https://doi.org/10.1016/S0168-8278(97)80160-2
  40. Thompson, K.C., A. Trowern, A. Fowell, M. Marathe, C. Haycock and M.J.P. Arthur. 1988. Primary rat and mouse hepatic stellate cells express the macrophage inhibitor cytokine interleukin-10 during the course of activation in vitro. Hepatology 28:1518-1524.
  41. Tracy, K.J., Y. Fong, D.G. Hesse, K.R. Manogue, A.T. lee and G.C. Kuo.1987. Anti-cachectin/TNF monoclonal antibodies prevent septic shock dmuring lethal bacteraemia. Nature 330:662-664. https://doi.org/10.1038/330662a0
  42. Van Snick, J. 1990. IL-6: an overview. Annu. Rev. Immunol. 8:253-278. https://doi.org/10.1146/annurev.iy.08.040190.001345
  43. Willeaume, V., V. Kruys, T. Mijatovic and G. Huez. 1996. Tumor necrosis factor-alpha production induced by viruses and by lipopolysaccharidesin macrophages: similarities and differences. J. Inflamm. 46:1-12.
  44. Yun, H.J., S.K. Heo, H.S. Yi, Kim CH, B.W. Kim and S.D. Park. 2008. Anti-inflammatory effect of Injinho-tang in RAW 264.7 Cells. Korean J. Herbology 23(2):169-178.

Cited by

  1. Ixeris dentata (Thunb. Ex Thunb.) Nakai Extract Inhibits Proliferation and Induces Apoptosis in Breast Cancer Cells through Akt/NF-κB Pathways vol.18, pp.12, 2017, https://doi.org/10.3390/ijms18020275
  2. Anti-inflammatory Activity of the Undaria pinnatifida Water Extract vol.55, pp.4, 2012, https://doi.org/10.3839/jabc.2012.035
  3. Induction of Apoptosis in Human Cancer Cells with Extracts of Taraxacum coreanum, Youngia sonchifolia and Ixeris dentate vol.31, pp.1, 2016, https://doi.org/10.13103/JFHS.2016.31.1.51
  4. Anti-inflammatory Effect of Ethanol Extract from Sargassum fulvellum on Lipopolysaccharide Induced Inflammatory Responses in RAW 264.7 Cells and Mice Ears vol.43, pp.8, 2014, https://doi.org/10.3746/jkfn.2014.43.8.1158
  5. Quantification of Major Compounds fromIxeris dentata,Ixeris dentataVar.albiflora,andIxeris sonchifoliaand Their Comparative Anti-Inflammatory Activity in Lipopolysaccharide-Stimulated RAW 264.7 Cells vol.18, pp.1, 2015, https://doi.org/10.1089/jmf.2014.3205
  6. Anti-inflammatory activity of ethanolic extract of Sargassum sagamianum in RAW 264.7 cells vol.22, pp.4, 2013, https://doi.org/10.1007/s10068-013-0191-9
  7. Anticancer effects of Ixeris dentata (Thunb. ex Thunb.) nakai extract on human melanoma cells A375P and A375SM vol.194, 2016, https://doi.org/10.1016/j.jep.2016.11.010
  8. Anti-inflammatory activity of manassantin A from ultra-fine ground Saururus chinensis in lipopolysaccharide-stimulated RAW 264.7 cells vol.60, pp.1, 2017, https://doi.org/10.1007/s13765-016-0249-5
  9. Anti-inflammatory Activity of Ethanol Extract of Undaria pinnatifida Root in RAW 264.7 Cells vol.47, pp.6, 2014, https://doi.org/10.5657/KFAS.2014.0751
  10. Inhibitory effect of epigallocatechin from Camellia sinensis leaves against pro-inflammatory mediator release in macrophages vol.60, pp.3, 2017, https://doi.org/10.3839/jabc.2017.032
  11. 씀바귀 추출물이 인체유방암세포의 활성 산소 및 Bcl-2 Family에 미치는 영향 vol.24, pp.6, 2014, https://doi.org/10.17495/easdl.2014.12.24.6.739
  12. 씀바귀, 질경이, 소리쟁이 에탄올 추출 혼합물에 대한 주요 성분 동시 분석 vol.51, pp.3, 2011, https://doi.org/10.22889/kjp.2020.51.3.222
  13. Responses of inflammation signaling pathway by saucerneol D from elicitor-treated Saururus chinensis on pro-inflammatory responses in LPS-stimulated Raw 264.7 cell vol.64, pp.1, 2011, https://doi.org/10.1186/s13765-020-00585-z