Journal of fish pathology (한국어병학회지)

The Korean Society of Fish Pathology (한국어병학회)
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Characteristics of Vibrio isolated from cultured file fish, Stephanolepis cirrhifer in Korea

우리나라 양식 쥐치, Stephanolepis cirrhifer에서 분리된 비브리오의 특성

  • Lee, Bo-Young (Aquatic life disease control division, National Fisheries Research and Development Institute) ;
  • Bang, Jong-Deuk (Aquatic life disease control division, National Fisheries Research and Development Institute) ;
  • Cho, Mi-Young (Aquatic life disease control division, National Fisheries Research and Development Institute) ;
  • Kim, Jin-Woo (Aquatic life disease control division, National Fisheries Research and Development Institute) ;
  • Won, Kyoung-Mi (Aquatic life disease control division, National Fisheries Research and Development Institute) ;
  • Cho, Young-Ah (Aquatic life disease control division, National Fisheries Research and Development Institute)
  • 지보영 (국립수산과학원 수산생물방역과) ;
  • 방종득 (국립수산과학원 수산생물방역과) ;
  • 조미영 (국립수산과학원 수산생물방역과) ;
  • 김진우 (국립수산과학원 수산생물방역과) ;
  • 원경미 (국립수산과학원 수산생물방역과) ;
  • 조영아 (국립수산과학원 수산생물방역과)
  • Received : 2011.07.05
  • Accepted : 2011.07.26
  • Published : 2011.08.31
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Abstract

An epidemic was occurred in Stephanolepis cirrhifer during acclimation in laboratory tanks (water temperature was about $17^{\circ}C$). Diseased fish showed an unique external sign, large cloudy skin surface. We investigated the cause of the disease, and isolated one pure cultured bacterium, that was identified as Vibrio anguillarum by biochemical analysis and sequence analysis of the l6S rRNA and recA genes. During the outbreak, about 30% of S. cirrhifer showed the large cloudy skin surface, and approximately 10% of the stocked fish died. Fish were effectively cured by treatment with oxolinic acid.

본 연구는 실험을 위해 저수온에서 순치 중이던 쥐치에서 체표의 백탁을 보이며 폐사하여 그 원인을 밝히고자 하였다. 순수 배양된 균체를 확인하여 계대 배양하여 생화학적 성상과 16S rRNA유전자와 recA 유전자의 염기서열 분석 결과 Vibrio anguillarum으로 나타났다. 빈사어 (체장 4~6 cm)의 약 30%는 체표 백탁 증상을 보였으며 약 10%는 폐사하였다. 이에 약제 감수성 시험 후, oxolinic acid 200 ppm을 1시간 동안 약욕시켰으며, 2일 경과 후 폐사가 중단되고 증상이 호전되었다.

Keywords

References

  1. Eddy, S.D. and Jones, S.H.: Microbiology of summer flounder Paralichthys dentatus fingerling production at a marine fish hatchery. Aquaculture, 211: 9-28, 2002. https://doi.org/10.1016/S0044-8486(01)00882-1
  2. Funakoshi, K., Atobe, Y., Nakano, M., Hisajima, T., Goris, R.C. and Kishida, R.: Monoaminergic and peptidergic axonal projections to the vagal motor cell column of a teleost, the filefish Stephanolepis cirrhifer. J. Comp. Neurol., 447(4): 351-365, 2002. https://doi.org/10.1002/cne.10238
  3. Gwag, S.N., Baeg, G.U. and Heo, S.H.: Feeding Habits of Stephanolepis cirrhifer in a Zostera marina Bed. Korean J. Ichthyol., 15(4): 219-223, 2003.
  4. Jang, H., Moon, J.S., Joh, S.J., Kim, J.Y. and Son, S.W.: Occurrence and treatment of mixed infections with Vibrio anguillarum and Ichthyobodo sp. from cultured oliver flounder(Paralichthys olivaceus). Korean J. Vet. Res., 47(3): 303-308, 2007.
  5. Kim, M.S., Seo, J.S., Park, M.A., Cho, J.Y., Hwang, J.Y., Kwon, M.G. and Jung, S.H.: Antimicrobial resistance of Edwardsiella tarda, Vibrio spp., and Streptococcus spp. isolated from olive flounder Paralichthys olivaceus. J. Fish Pathol., 23(1): 37-45, 2010.
  6. Kim, S.M., Won, K.M., Woo, S.H., Li, H., Kim, E.J., Choi, K.J., Cho, M.Y., Kim, M.S. and Park, S.I.: Vibrios Isolated from Diseased Marine Culturing Fishes in Korea. J. Fish Pathol., 18(2): 133-145, 2005.
  7. Krawiec, S. and Riley, M.: Organization of the bacterial chromosome. Microbiol. Rev., 54: 502-539, 1990.
  8. Lee, S.J., Go, Y.B. and Lee, Y.D.: Annual reproductive cycle of the file fish, Stephanolepis cirrhifer, on the southern coast of Cheju Island. Korean J. Ichthyol., 12(1): 62-70, 2000.
  9. Lunder, T., Sorum, H., Holstad, G., Steigerwalt, A.G., Mowinckel, P. and Brenner, D.J.: Phenotypic and genotypic charcterization of Vibrio viscosus sp. nov. and Vibrio wodanis sp. nov. isolated from Atlantic salmon(Salmon salar) with 'winter ulcer'. Int. J. Syst. Evol. Microbiol., 50: 427-450, 2000. https://doi.org/10.1099/00207713-50-2-427
  10. MacFaddin, J.E.: Individual biochemical tests. In: Biochemical tests for identification of medical bacteria. 3rd ed., Lippincott Williams and Wilkins, Philadelphia, pp. 1-456, 2000.
  11. Miyajima, Y., Masuda, R., Kurihara, A., Kamata, R., Yamashita, Y. and Takeuchi, T.: Juveniles of threadsail filefish, Stephanolepis cirrhifer, can survive and grow by feeding on moon jellyfish Aurelia aurita. Fish Sci., 77: 41-48, 2011. https://doi.org/10.1007/s12562-010-0305-8
  12. Mo, Z.L., Tan, X.G., Xu, Y.L. and Zhang, P.J.: A Vibrio anguillarum strain associated with skin ulcer on cultured flounder, paralichthys olivaceus. Chinese J. Oceanol. Limnol., 19(4): 319-326, 2001. https://doi.org/10.1007/BF02850735
  13. Muroga, K., Tanasoomwang, V. and Momoyama, K.: Vibrio anguillarum infection in juveniles of tiger puffer(Takifugu rubripes). Fish Pathol., 22: 29-30, 1987. https://doi.org/10.3147/jsfp.22.29
  14. Muroga, K. and Tatani, M.: Vibrio anguillarum from juvenile red sea-bream(Pagrus major). Fish Pathol., 16: 211-214, 1982. https://doi.org/10.3147/jsfp.16.211
  15. Muroga, K., Iida, M., Masumoto, H. and Nakai, T.: Detection of Vibrio anguillarum from water. Nippon Suisan Gakkaishi, 52: 641-647, 1986. https://doi.org/10.2331/suisan.52.641
  16. Pirarat, N., Katagiri, T., Maita, M., Nakai, T., Endo, M.: Viral encephalopathy and retinopathy in hatchery-reared juvenile thread-sail filefish(Stephanolepis cirrhifer). Aquaculture, 288(3-4): 329-352, 2009.
  17. Rubin, S.J. and Tilton, R.C.: Isolation of Vibrio alginolyticus from Wound infections. J. Clin. Microbiol., 2(6): 556-558, 1975.
  18. Sugita, H., Mizuki, H. and Itoi, S.: Prevalence of a fish pathogen, Listonella anguillarum, in the intestinal tract of fish collected off the coast of Japan. Aquaculture Res., 39: 103-105, 2008.
  19. Toranzo, A.E. and Barja, J.L.: A review of the taxonomy and seroepizootiology of Vibrio anguillarum, with special reference to aquaculture in the northwest of Spain. Dis. Aquat. Org., 9: 73-82, 1990. https://doi.org/10.3354/dao009073
  20. Wang, C.Y., Yan, M.C., Chen, S.B., Guan, M.X., Shan, L.Z., Al, W.M., Xie, Q.L. and Cai, Y.B.: Isolation and identification of Listonella anguillarum from Plecoglossus altivelis based on 16S rRNA and recA. Mar. Sci. Bull., 29(1): 84-90, 2010.
  21. Yamanoi, H., Momoyama, K., Yasunobu, H. and Muroga, K.: Vibrio anguillarum infection in founder(Paralichthys olivaceus) fingerings. Fish Pathol., 23: 69-70, 1988. https://doi.org/10.3147/jsfp.23.69
  22. Zorrilla, I., Chabrillon, M., Arijo, S., Diaz-Rosales, P., Martinez-Manzanares, E., Balebona, M.C. and Morinigo M.A.: Bacteria recovered from diseased cultured gilthead sea bream(Sparus aurata L.) in southwestern Spain. Aquaculture, 218: 11-20, 2003. https://doi.org/10.1016/S0044-8486(02)00309-5
  23. 명정구, 김병일, 이선명, 전길봉과 낚시춘추: 우리바다 어류도감, p.234, (4), 예조원, 경기도, 2007.
  24. 해양수산부(연구기관: 동우대학): 쥐치종묘 대량생산 기술개발 연구보고서, p.3, 2001.
  25. 통계청: 2006-2009, 어류양식동향조사: 시도.시군구별 양식방법별 어종별 양식현황[온라인], http://kostat.go.kr/portal/korea/index.action[인용일자: 2011.6.23]
  26. 국립수산과학원, 2009-2010, 실시간어장정보: 관측정보 [온라인], http://portal.nfrdi.re.kr/risa/subpage/realtime04.jsp [인용일자: 2011.7.1]

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