FLOWER RESEARCH JOURNAL (화훼연구)

The Korean Society for Floricultural Science (한국화훼학회)
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Fragrance Analysis Using GC-MS and Electronic Nose in Phalaenopsis

GC-MS와 전자코를 이용한 팔레놉시스 향기 분석

  • Park, PueHee (Floriculture Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Yae, ByeongWoo (Floriculture Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Kim, MiSeon (Floriculture Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Lee, YoungRan (Floriculture Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Park, PilMan (Floriculture Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Lee, DongSoo (Floriculture Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration)
  • 박부희 (국립원예특작과학원 화훼과) ;
  • 예병우 (국립원예특작과학원 화훼과) ;
  • 김미선 (국립원예특작과학원 화훼과) ;
  • 이영란 (국립원예특작과학원 화훼과) ;
  • 박필만 (국립원예특작과학원 화훼과) ;
  • 이동수 (국립원예특작과학원 화훼과)
  • Received : 2011.08.12
  • Accepted : 2011.11.03
  • Published : 2011.12.30
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Abstract

Phalaenopsis (P.) has various species, and some of them have strong fragrance. There are fragrant species such as P. bellina, P. violacea, P. schilleriana and used in breeding program for fragrant Phalaenopsis. This study was performed for establishment of fragrance analysis system using GC-MS and electronic nose in eight P. resources. We analyzed fragrant compound using the tissue of sepal, petal, column, and lip of P. '3010'. The percentage of the major compound was high in the petal and lip tissues. The main compound emitted from P. bellina was linalool (21.21%). It was possible that fragrance pattern could be analyzed among the resources using the electronic nose. Discriminant function analysis (DFA) was more useful than the principal component analysis (PCA) in statistics program. We utilized GC-MS method for the major compounds of flower from our breeding materials. This study would be useful to the fragrant analysis system for the fragrant orchid breeding in the future.

팔레놉시스는 다양한 종을 가지고 있고, 강한 향기를 보유하고 있는 종들이 존재한다. 팔레놉시스 원종 중 P. bellina, P. violacea, P. schilleriana 등 소수 계통들은 향을 보유하고 있어 방향성 육종을 위한 재료로 이용된다. 본 연구는 팔레놉시스 방향성 육종을 위한 효율적인 향기 분석 시스템을 확립하고자 팔레놉시스 유전자원 8종류에 대하여 GC-MS와 전자코를 이용하여 향기 분석을 수행하였다. 팔레놉시스 '3010'에 대해서는 화기 조직별 즉 꽃받침, 꽃잎, 주두, 설판으로 나누어 분석하였다. 주된 성분비가 꽃잎과 설판 조직에서 높게 나타났다. P. bellina 계통의 주된 성분은 linalool(21.21%)이었다. 전자코를 이용하여 각 계통간 향기성분의 패턴 분석이 가능하였다. 통계 프로그램에서는 PCA 보다는 DFA 분석이 더 유용하였다. 방향성 육종 시 보유하는 육종 재료들의 주된 향기 구성성분을 알고자 GC-MS를 이용하였다. 본 연구는 향후 난의 방향성 육종을 위한 향기 분석시스템에 유용하게 활용될 것이다.

Keywords

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