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The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Effects of Resveratrol on Migration and Proliferation in HT-29 Colon Cancer Cells

레스베라트롤의 HT-29 대장암 세포증식 및 이동성 억제효과

  • Lee, Sol Hwa (Department of Biological Sciences and Biotechnology, College of Life Science and Nano Technology, Hannam University) ;
  • Park, Song Yi (Department of Biological Sciences and Biotechnology, College of Life Science and Nano Technology, Hannam University) ;
  • Kim, In-Seop (Department of Biological Sciences and Biotechnology, College of Life Science and Nano Technology, Hannam University) ;
  • Park, Ock Jin (Department of Food and Nutrition, College of Life Science and Nano Technology, Hannam University) ;
  • Kim, Young Min (Department of Biological Sciences and Biotechnology, College of Life Science and Nano Technology, Hannam University)
  • 이솔화 (한남대학교 생명나노과학대학 생명시스템과학과) ;
  • 박송이 (한남대학교 생명나노과학대학 생명시스템과학과) ;
  • 김인섭 (한남대학교 생명나노과학대학 생명시스템과학과) ;
  • 박옥진 (한남대학교 생명나노과학대학 식품영양학과) ;
  • 김영민 (한남대학교 생명나노과학대학 생명시스템과학과)
  • Received : 2012.07.30
  • Accepted : 2012.10.10
  • Published : 2012.10.31
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Abstract

Resveratrol, natural polyphenol in grapes and red wine, is known to have the anti-proliferatory and anti-angiogenic effects in various cancer cells. In this study, we have investigated the effects of resveratrol in HT-29 colon cancer cells. Treatment of resveratrol in different concentrations and time inhibited proliferation of HT-29 colon cancer cells. We explored the effects of resveratrol on HT-29 colon cancer cell motility using a wound healing assay. In the absence of the resveratrol, the HT-29 cells are migrated along the edges of the wound and showed a large-scale migration, whereas dose- and time-dependent inhibition of cell flattening and spreading was observed in the presence of resveratrol. Resveratrol inhibited MMP-9 in a dose- and time-dependent on HT-29 colon cancer cells by Western blotting. In addition, resveratrol increased AMPK activity and decreased COX-2, VASP and VEGF expression. Treatment of compound C inhibited AMPK activity, however, the expression of VASP and COX-2 increased thus, COX-2 and VASP are modulated by AMPK. However treatment of celecoxib could not control AMPK activity but decreased VEGF expression. We suggest that resveratrol inhibits cell proliferation and migration through activation of AMPK and decreased COX-2, VASP and VEGF expression in HT-29 colon cancer cells.

Keywords

References

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