Asian Pacific Journal of Cancer Prevention

Asian Pacific Journal of Cancer Prevention (아시아태평양암예방학회)
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Tanshinone II-A Inhibits Angiogenesis through Down Regulation of COX-2 in Human Colorectal Cancer

  • Zhou, Li-Hong (Clinic Oncology, Putuo Hospital & Cancer Institute, Shanghai University of Traditional Chinese Medicine) ;
  • Hu, Qiang (Department of Genernal Surgery, Dahua Hospital) ;
  • Sui, Hua (Clinic Oncology, Putuo Hospital & Cancer Institute, Shanghai University of Traditional Chinese Medicine) ;
  • Ci, Shu-Jun (Clinic Oncology, Putuo Hospital & Cancer Institute, Shanghai University of Traditional Chinese Medicine) ;
  • Wang, Yan (Clinic Oncology, Putuo Hospital & Cancer Institute, Shanghai University of Traditional Chinese Medicine) ;
  • Liu, Xuan (Clinic Oncology, Putuo Hospital & Cancer Institute, Shanghai University of Traditional Chinese Medicine) ;
  • Liu, Ning-Ning (Clinic Oncology, Putuo Hospital & Cancer Institute, Shanghai University of Traditional Chinese Medicine) ;
  • Yin, Pei-Hao (Department of Genernal Surgery, Putuo Hospital, Shanghai University of Traditional Chinese Medicine) ;
  • Qin, Jian-Min (Department of Genernal Surgery, Putuo Hospital, Shanghai University of Traditional Chinese Medicine) ;
  • Li, Qi (Clinic Oncology, Putuo Hospital & Cancer Institute, Shanghai University of Traditional Chinese Medicine)
  • Published : 2012.09.30
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Abstract

Angiogenesis plays a significant role in colorectal cancer (CRC) and cyclooxygenase-2 (COX-2) appears to be involved with multiple aspects of CRC angiogenesis. Our aim was to investigate the inhibitory effects of Tan II-A (Tanshinone II-A, Tan II-A) on tumor growth in mice, as well as alteration of expression of COX-2 and VEGF in CRC. We established the mice xenograft model of C26 CRC cell line, and injected 0.5, 1, 2mg/kg of Tan II-A and 1mg/kg of 5-FU in respectively in vivo. Then, we assayed tumor weight and volume, and evaluated microvascular density and expression of VEGF. COX-2 promoter and COX-2 plasmids were transfected into HCT-116 cells, followed by detection of COX-2 promoter activity by chemiluminescence, and detection of COX-2 mRNA expression by fluorescence quantitative PCR. Taken together, the results showed Tan II-A could inhibit tumor growth and suppress the VEGF level in vivo. HCT-116 cell experiments showed marked inhibitory effects of Tan II-A on COX-2 and VEGF in a dose-dependent manner. The results indicate that Tan II-A can effectively inhibit tumor growth and angiogenesis of human colorectal cancer via inhibiting the expression level of COX-2 and VEGF.

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References

  1. Arentz G, Chataway T, Price TJ, et al (2011). Desmin expression in colorectal cancer stroma correlates with advanced stage disease and marks angiogenic microvessels. Clin Proteomics, 8, 16. https://doi.org/10.1186/1559-0275-8-16
  2. Amini A, Moghaddam SM, Morris DL, et al (2012). The Critical Role of Vascular Endothelial Growth Factor in Tumor Angiogenesis. Curr Cancer Drug Targets, 12, 23-43. https://doi.org/10.2174/156800912798888956
  3. Chen J, Shi DY, Liu SL, et al (2012). Tanshinone IIA induces growth inhibition and apoptosis in gastric cancer in vitro and in vivo. Oncol Rep, 27, 523-8.
  4. Casimiro S, Luis I, Fernandes A, et al (2012). Analysis of a bone metastasis gene expression signature in patients with bone metastasis from solid tumors. Clin Exp Metastasis, 29, 155-64. https://doi.org/10.1007/s10585-011-9438-0
  5. Chien SY, Kuo SJ, Chen YL, et al (2012). Tanshinone IIA inhibits human hepatocellular carcinoma J5 cell growth by increasing Bax and caspase 3 and decreasing CD31 expression in vivo. Mol Med Report, 5, 282-6.
  6. Dai ZK, Qin JK, Huang JE, et al (2012). Tanshinone IIA activates calcium-dependent apoptosis signaling pathway in human hepatoma cells. J Nat Med, 66, 192-201. https://doi.org/10.1007/s11418-011-0576-0
  7. Dohadwala M, Yang SC, Luo J, et al (2006). Cyclooxygenase-2 dependent regulation of E-cadherin: prostaglandin E(2) induces transcriptional repressors ZEB1 and snail in nonsmall cell lung cancer. Cancer Res, 66, 5338-45. https://doi.org/10.1158/0008-5472.CAN-05-3635
  8. Jemal A, Bray F, Center MM, et al (2011). Global cancer statistics. CA Cancer J Clin, 61, 69-90. https://doi.org/10.3322/caac.20107
  9. Folkman J (1974). Tumor angiogenesis. Adv Cancer Res, 0, 331-58.
  10. Franchi A, Gallo O, Massi D, et al (2004). Tumor lymphangiogenesis in head and neck squamous cell carcinoma: a morphometric study with clinical correlations. Cancer, 101, 973-8. https://doi.org/10.1002/cncr.20454
  11. Itatsu K, Sasaki M, Yamaguchi J, et al (2009). Cyclooxygenase-2 is involved in the up-regulation of matrix metalloproteinase-9 in cholangiocarcinoma induced by tumor necrosis factoralpha. Am J Pathol, 174, 829-41. https://doi.org/10.2353/ajpath.2009.080012
  12. Karahan N, Güney M, Baspinar S, et al (2007). Expression of gelatinase (MMP-2 and MMP-9) and cyclooxygenase-2 (COX-2) in endometrial carcinoma. Eur J Gynaecol Oncol, 28, 184-8.
  13. Lazar D, Taban S, Ardeleanu C, et al (2008). Immunohistochemical expression of the cyclooxygenase-2 (COX-2) in gastric cancer. The correlations with the tumor angiogenesis and patients' survival. Rom J Morphol Embryol, 49, 371-9.
  14. Tsujii M, Kawano S, Tsuji S, et al (1998). Cyclooxygenase regulates angiogenesis induced by colon cancer cells. Cell, 93, 705-16. https://doi.org/10.1016/S0092-8674(00)81433-6
  15. Wang J, Wang X, Jiang S, et al (2007). Growth inhibition and induction of apoptosis and differentiation of tanshinone IIA in human glioma cells. J Neurooncol, 82, 11-21. https://doi.org/10.1007/s11060-006-9242-x
  16. Waldner MJ, Neurath MF (2012). Targeting the VEGF signaling pathway in cancer therapy. Expert Opin Ther Targets, 16, 5-13. https://doi.org/10.1517/14728222.2011.641951
  17. Zhang K, Li J, Meng W, et al (2010). $C/EBP\beta$ and CHOP participate in tanshinone IIA-induced differentiation and apoptosis of acute promyelocytic leukemia cells in vitro. Int J Hematol, 92, 571-8. https://doi.org/10.1007/s12185-010-0686-6
  18. Zhang Y, Wei RX, Zhu XB, et al (2012). Tanshinone IIA induces apoptosis and inhibits the proliferation, migration, and invasion of the osteosarcoma MG-63 cell line in vitro. Anticancer Drugs, 23, 212-9. https://doi.org/10.1097/CAD.0b013e32834e5592

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