Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Effect of Cordycepin Purified from Cordyceps militaris on Th1 and Th2 Cytokines in Mouse Splenocytes

  • Jeong, Min-Ho (Department of Microbiology, Dong-A University College of Medicine) ;
  • Seo, Min Jeong (Medi-Farm Industrialization Research Center, Dong-A University) ;
  • Park, Jeong Uck (Medi-Farm Industrialization Research Center, Dong-A University) ;
  • Kang, Byoung Won (Medi-Farm Industrialization Research Center, Dong-A University) ;
  • Kim, Kyoung-Sook (Medi-Farm Industrialization Research Center, Dong-A University) ;
  • Lee, Jae Yun (Cheongweon-Industrial Farm) ;
  • Kim, Gi-Young (Laboratory of Immunobiology, Department of Marine Life Sciences, Jeju National University) ;
  • Kim, Jung-In (School of Food and Life Sciences, Inje University) ;
  • Choi, Yung Hyun (Department of Biochemistry, College of Oriental Medicine, Dong-Eui University) ;
  • Kim, Kwang Hyuk (Department of Microbiology, Kosin University College of Medicine) ;
  • Jeong, Yong Kee (Department of Microbiology, Dong-A University College of Medicine)
  • Received : 2012.03.15
  • Accepted : 2012.03.31
  • Published : 2012.08.28
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Abstract

Cordycepin was purified from a mushroom, Cordyceps militaris, and its effect on Th1 and Th2 cytokines was examined. The level of cytokine induction in mouse splenocytes was estimated after co-inoculation of purified cordycepin and LPS. When $5{\mu}g/ml$ of purified cordycepin was exposed to mouse splenocytes for 72 h, the level of a Th1 cytokine IL-12 increased by 2.9-fold. The addition of the purified cordycepin to splenocytes also increased the level of Th2 cytokines, IL-4 and IL-10, by 1.9- and 1.8-fold, respectively. Therefore, cordycepin increases the cytokine levels and may contribute to the up-regulation of cellular and humoral immunity.

Keywords

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