Journal of Nutrition and Health

The Korean Nutrition Society (한국영양학회)
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A comparison of anti-inflammatory activities of green tea and grapefruit seed extract with those of microencapsulated extracts

미세캡슐화한 녹차 및 자몽종자 추출물이 Murine RAW 264.7 대식세포주의 항염증에 미치는 영향 비교

  • Received : 2012.10.02
  • Accepted : 2012.10.19
  • Published : 2012.10.31
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Abstract

We compared the effects of grapefruit seed extract (GFSE), green tea extract (GT) and their microencapsulated extract on anti-inflammatory activities in murine RAW 264.7 macrophages cell line. In order to protect the bioactive compounds in the extracts, they were microencapsulated with maltodextrin and $H_2O$. Nitric oxide (NO), prostaglandin E2 (PGE2), tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$), inducible nitric oxide synthase (iNOS) protein expression and thiobarbiturate reactive substances (TBARS) were analyzed in LPS activated RAW 264.7 macrophages. The green tea extract at the range of $100-600{\mu}g/mL$ inhibited NO, PGE2 production and iNOS protein expression without cytotoxicity in a dose-dependent manner. Grapefruit seed extract had strong inhibitory effects on NO and PGE production and iNOS protein expression at the range of $5-20{\mu}g/mL$ without cytotoxicity. Microencapsulation of green tea extract had further inhibitory effects on NO and PGE2 production and on iNOS protein expression, whereas microencapsulated GFSE did not show any further inhibitory effects on these parameters. Taken together, our results suggest that GSFE might be a promising candidate for preventing inflammation related diseases, such as cardiovascular disease, cancer or diabetes, and the microencapsulation of green tea extract could improve its bioactivity.

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References

  1. Sies H. Oxidative stress: oxidants and antioxidants. Exp Physiol 1997; 82(2): 291-295
  2. Byers T, Perry G. Dietary carotenes, vitamin C, and vitamin E as protective antioxidants in human cancers. Annu Rev Nutr 1992; 12: 139-159 https://doi.org/10.1146/annurev.nu.12.070192.001035
  3. Bogdan C, Rollinghoff M, Diefenbach A. The role of nitric oxide in innate immunity. Immunol Rev 2000; 173(1): 17-26 https://doi.org/10.1034/j.1600-065X.2000.917307.x
  4. Evans CH. Nitric oxide: what role does it play in inflammation and tissue destruction? Agents Actions Suppl 1995; 47: 107-116
  5. Grosjean SA, Arstall MA, Mitchell RN, Klappacher GW, Kelly RA, Pfeffer MA, Pfeffer JM. Inducible nitric oxide synthase and tumor necrosis factor in animal models of myocardial necrosis induced by coronary artery ligation or isoproterenol injection. J Card Fail 1999; 5(3): 236-245 https://doi.org/10.1016/S1071-9164(99)90008-8
  6. Crook MA, Tutt P, Pickup JC. Elevated serum sialic acid concentration in NIDDM and its relationship to blood pressure and retinopathy. Diabetes Care 1993; 16(1): 57-60 https://doi.org/10.2337/diacare.16.1.57
  7. Nathan CF, Hibbs JB Jr. Role of nitric oxide synthesis in macrophage antimicrobial activity. Curr Opin Immunol 1991; 3(1): 65-70 https://doi.org/10.1016/0952-7915(91)90079-G
  8. Tang SY, Whiteman M, Jenner A, Peng ZF, Halliwell B. Mechanism of cell death induced by an antioxidant extract of Cratoxylum cochinchinense (YCT) in Jurkat T cells: the role of reactive oxygen species and calcium. Free Radic Biol Med 2004; 36(12): 1588-1611 https://doi.org/10.1016/j.freeradbiomed.2004.03.018
  9. Ebrahimzadeh MA, Pourmorad F, Bekhradnia AR. Iron chelating activity, phenol and flavonoid content of some medicinal plants from Iran. Afr J Biotechnol 2008; 7(18): 3188-3192
  10. Hertog MG, Feskens EJ, Hollman PC, Katan MB, Kromhout D. Dietary antioxidant flavonoids and risk of coronary heart disease: the Zutphen Elderly Study. Lancet 1993; 342(8878): 1007-1011 https://doi.org/10.1016/0140-6736(93)92876-U
  11. Mirmiran P, Noori N, Zavareh MB, Azizi F. Fruit and vegetable consumption and risk factors for cardiovascular disease. Metab olism 2009; 58(4): 460-468 https://doi.org/10.1016/j.metabol.2008.11.002
  12. Proteggente AR, Pannala AS, Paganga G, Van Buren L, Wagner E, Wiseman S, Van De Put F, Dacombe C, Rice-Evans CA. The antioxidant activity of regularly consumed fruit and vegetables reflects their phenolic and vitamin C composition. Free Radic Res 2002; 36(2): 217-233 https://doi.org/10.1080/10715760290006484
  13. Dillard CJ, German JB. Phytochemicals: nutraceuticals and human health. J Sci Food Agric 2000; 80(12): 1744-1756 https://doi.org/10.1002/1097-0010(20000915)80:12<1744::AID-JSFA725>3.0.CO;2-W
  14. Al-Saikhan MS, Howard LR, Miller JC. Antioxidant activity and total phenolics in different genotypes of potato (Solanum tuberosum, L.). J Food Sci 1995; 60(2): 341-343 https://doi.org/10.1111/j.1365-2621.1995.tb05668.x
  15. Vinson JA, Su X, Zubik L, Bose P. Phenol antioxidant quantity and quality in foods: fruits. J Agric Food Chem 2001; 49(11): 5315-5321 https://doi.org/10.1021/jf0009293
  16. Singh R, Akhtar N, Haqqi TM. Green tea polyphenol epigallocatechin- 3-gallate: inflammation and arthritis. [corrected]. Life Sci 2010; 86(25-26): 907-918 https://doi.org/10.1016/j.lfs.2010.04.013
  17. Serafini M, Ghiselli A, Ferro-Luzzi A. In vivo antioxidant effect of green and black tea in man. Eur J Clin Nutr 1996; 50(1): 28-32
  18. Conney AH, Wang ZY, Huang MT, Ho CT, Yang CS. Inhibitory effect of green tea on tumorigenesis by chemicals and ultraviolet light. Prev Med 1992; 21(3): 361-369 https://doi.org/10.1016/0091-7435(92)90043-H
  19. Ishikawa T, Suzukawa M, Ito T, Yoshida H, Ayaori M, Nishiwaki M, Yonemura A, Hara Y, Nakamura H. Effect of tea flavonoid supplementation on the susceptibility of low-density lipoprotein to oxidative modification. Am J Clin Nutr 1997; 66(2): 261-266
  20. Keevil JG, Osman HE, Reed JD, Folts JD. Grape juice, but not orange juice or grapefruit juice, inhibits human platelet aggregation. J Nutr 2000; 130(1): 53-56
  21. Yue X, Xu Z. Changes of anthocyanins, anthocyanidins, and antioxidant activity in bilberry extract during dry heating. J Food Sci 2008; 73(6): C494-C499 https://doi.org/10.1111/j.1750-3841.2008.00845.x
  22. Francis FJ. Food colorants: anthocyanins. Crit Rev Food Sci Nutr 1989; 28(4): 273-314 https://doi.org/10.1080/10408398909527503
  23. Davies AJ, Mazza G. Copigmentation of simple and acylated anthocyanins with colorless phenolic-compounds. J Agric Food Chem 1993; 41(5): 716-720 https://doi.org/10.1021/jf00029a007
  24. Main JH, Clydesdale FM, Francis FJ. Spray drying anthocyanin concentrates for use as food colorants. J Food Sci 1978; 43(6): 1693-1694 https://doi.org/10.1111/j.1365-2621.1978.tb07390.x
  25. Ersus S, Yurdagel U. Microencapsulation of anthocyanin pigments of black carrot (Daucus carota L.) by spray drier. J Food Eng 2007; 80(3): 805-812 https://doi.org/10.1016/j.jfoodeng.2006.07.009
  26. Takahashi M, Uechi S, Takara K, Asikin Y, Wada K. Evaluation of an oral carrier system in rats: bioavailability and antioxidant properties of liposome-encapsulated curcumin. J Agric Food Chem 2009; 57(19): 9141-9146 https://doi.org/10.1021/jf9013923
  27. Lee HD, Kim HS, Seong PN, Kim MH. Effect of microencapsulation processing on functional property of natural pigment and antioxidant. Food Eng Prog 2012; 16(3): 283-286
  28. Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 1983; 65(1-2): 55-63 https://doi.org/10.1016/0022-1759(83)90303-4
  29. Green LC, Wagner DA, Glogowski J, Skipper PL, Wishnok JS, Tannenbaum SR. Analysis of nitrate, nitrite, and [15N]nitrate in biological fluids. Anal Biochem 1982; 126(1): 131-138 https://doi.org/10.1016/0003-2697(82)90118-X
  30. Radkar V, Hardej D, Lau-Cam C, Billack B. Evaluation of resveratrol and piceatannol cytotoxicity in macrophages, T cells, and skin cells. Arh Hig Rada Toksikol 2007; 58(3): 293-304
  31. Smith JR, Hart PH, Williams KA. Basic pathogenic mechanisms operating in experimental models of acute anterior uveitis. Immunol Cell Biol 1998; 76(6): 497-512 https://doi.org/10.1046/j.1440-1711.1998.00783.x
  32. Chiou WF, Chen CF, Lin JJ. Mechanisms of suppression of inducible nitric oxide synthase (iNOS) expression in RAW 264.7 cells by andrographolide. Br J Pharmacol 2000; 129(8): 1553-1560 https://doi.org/10.1038/sj.bjp.0703191
  33. Tsai PJ, Tsai TH, Yu CH, Ho SC. Comparison of NO-scavenging and NO-suppressing activities of different herbal teas with those of green tea. Food Chem 2007; 103(1): 181-187 https://doi.org/10.1016/j.foodchem.2006.08.013
  34. Nakagawa T, Yokozawa T. Direct scavenging of nitric oxide and superoxide by green tea. Food Chem Toxicol 2002; 40(12): 1745-1750 https://doi.org/10.1016/S0278-6915(02)00169-2
  35. Paquay JB, Haenen GR, Stender G, Wiseman SA, Tijburg LB, Bast A. Protection against nitric oxide toxicity by tea. J Agric Food Chem 2000; 48(11): 5768-5772 https://doi.org/10.1021/jf981316h
  36. Mandadi KK, Jayaprakasha GK, Bhat NG, Patil BS. Red Mexican grapefruit: a novel source for bioactive limonoids and their antioxidant activity. Z Naturforsch C 2007; 62(3-4): 179-188
  37. Kaur M, Agarwal CH, Agarwal R. Anticancer and cancer chemopreventive potential of grape seed extract and other grpaebased product. J Nutr 2009;139:1806S-1812S https://doi.org/10.3945/jn.109.106864
  38. Bagchi D, Garg A, Krohn RL, Bagchi M, Tran MX, Stohs SJ. Oxygen free radical scavenging abilites of vitamin C and E, and a grape seed proanthocyanidin extract in vitro. Res Commun Mol Pathos Pharmacol 1997; 95: 179-189
  39. Pedrielli P, Pedulli GF, Skibsted LH. Antioxidant mechanism of flavonoids. Solvent effect on rate constant for chain-breaking reaction of quercetin and epicatechin in autoxidation of methyl linoleate. J Agric Food Chem 2001; 49(6): 3034-3040 https://doi.org/10.1021/jf010017g
  40. Yu J. Citrus limonoids and flavonoids: extraction, antioxidant activity and effects on hamster plasma cholesterol distribution [dissertation]. College Station (TX): Texas A & M University; 2004
  41. Halliwell B. Are polyphenols antioxidants or pro-oxidants? What do we learn from cell cultrue and in vivo studies? Arc Biochem Biophys 2008; 476: 107-112 https://doi.org/10.1016/j.abb.2008.01.028
  42. McClements DJ. Design of nano-laminated coatings to control bioavailability of lipophilic food components. J Food Sci 2010; 75(1): R30-R42 https://doi.org/10.1111/j.1750-3841.2009.01452.x
  43. Fang Z, Bhandari B. Encapsulation of polyphenols-a review. Trends Food Sci Technol 2010; 21(10): 510-523 https://doi.org/10.1016/j.tifs.2010.08.003
  44. Zhang L, Mou D, Du Y. Procyanidins: extraction and micro-encapsulation. J Sci Food Agric 2007; 87(12): 2192-2197 https://doi.org/10.1002/jsfa.2899
  45. Aoshima H, Ayabe S. Prevention of the deterioration of polyphenol-rich beverages. Food Chem 2007; 100:350-355 https://doi.org/10.1016/j.foodchem.2005.09.052

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