DOI QR코드

DOI QR Code

Isolation and Indenfication of Acid-forming Bacteria from a Fresh Wheat Makgeolli in Jeonju

전주 생 밀 막걸리의 산패 원인균의 분리 및 동정

  • Park, Soon-Hi (Dept. of Alternative Medicine and Alternative Medicine, Jeonju University) ;
  • Lee, Seul (Dept. of Alternative Medicine and Alternative Medicine, Jeonju University) ;
  • Jin, Hyo-Sang (Dept. of Alternative Medicine and Alternative Medicine, Jeonju University)
  • 박순희 (전주대학교 대체의학대학원 영양식품요법) ;
  • 이슬 (전주대학교 대체의학대학원 영양식품요법) ;
  • 진효상 (전주대학교 대체의학대학원 영양식품요법)
  • Received : 2012.10.19
  • Accepted : 2012.12.04
  • Published : 2012.12.31

Abstract

This research was carried out in order to discover acid-forming bacteria during fermentation of Makgeolli, as Makgeolli loses its commercial value due to overproduced acidic materials. In Makgeolli kept at $25^{\circ}C$, a sudden increase of acidity as well as the disappearance of yeast cells occurred at day 6, whereas the total cell count and bacterial type remained unchanged; the result implies that a succession of bacterial types, including acid forming bacteria, occurred. Two acid-forming bacteria were isolated from acidified Makgeolli and were identified as Acetobacter pasteurianus and Lactobacillus casei. When fresh and heat-treated Makgeolli were inoculated with Acetobacter pasteurianus and/or Lactobacillus casei, the greatest amount of acid was formed in Makgeolli inoculated with Acetobacter pasteurianus and Lactobacillus casei and also in Makgeolli with Acetobacter pasteurianus alone. This result indicates that Acetobacter pasteurianus is the main acidifier; furthermore, it shows the synergy effect in acid formation with Lactobacillus casei.

Keywords

References

  1. Blast. http://blast.ncbi.nlm.nih.gov. 2010.9.3 방문
  2. Cha YJ, Park KJ, Kim DK, Chun HS, Lee BK, Kim KH, Lee SY, Kim SJ. 1994. Isolation and characterization of cellulose producing Acetobacter xylinum KI strain. Korean J Appl Microbiol Biotechnol 22:571-576
  3. Chang JH. 1989. History of Korean spirits. Korean J Dietary Culture 4:271-274
  4. Hong HG. 1984. The effect on the composition of takju of addition of nuruk under varied conditions. MS Thesis, Seoul Women's University. Seoul. Korea
  5. J Ames M Jay. 2000. Modern Food Microbiology. 6th ed. pp. 149-152. APAC Pub.
  6. Jung BK. 2007. Studies on Takju brewing with red yeast rice. MS Thesis, Seoul National University of Science and Technology. Seoul. Korea
  7. Jung EK, Kim SJ, Joo NM. 2011. Consumption and satisfaction evaluation of Takju among consumers ages 20 to 29. J Korean Diet Assoc 17:47-57
  8. Jwa MK, Lim SB, Song DJ, Kim BO. 2000. Quality changes of commercial Yakju and Takju during storage. Cheju Journal of Life Science 3:3-9
  9. Kim JH, Lee SJ, Jung YW, Park SY, Shin WC, Park CS, Hong SY, Kim GW. 2011. Composition of organic acids and physiological functionality of commercial Makgeolli. Korean J Food Sci Tec 43:206-212 https://doi.org/10.9721/KJFST.2011.43.2.206
  10. Kim JH, Lee SY, Kim KBWR, Song EJ, Kim AR, Kim MJ, Ji KW, Ahn IS, AH DH. 2007. Effects of Glycyrrhiza uralensis, Menthae herba, Schizandra chinensis and chitosan on the shelf-life and quality of Takju. J Korean Soc Food Sci Nutr 36:1436-1443 https://doi.org/10.3746/jkfn.2007.36.11.1436
  11. Kim MY. 2011. Effect of inoculation level of starter culture (Acetobacter aceti) and acetic acid addition on fermentation of Makgeolli vinegar. MS Thesis, Konkuk University. Seoul. Korea
  12. Kirsop BE, Snell JJS. 1984. Maintenance of Microorganisms. pp. 35-40. Academic Press. London
  13. Lee CH, Kim GM. 1995. Determination of the shelf-life of pasteurized Korean rice wine, Yakju, in aseptic packaging. Korean J Food Sci Technol 27:156-163
  14. Lee HO. 2008. Studies on Takju brewing with birch sap. MS Thesis, Seoul National University of Science and Technology. Seoul. Korea
  15. Lee JS, Lee TW. 1970. Studies on the microflora of Takju brewing. Korean J Microbiol 8:116-133
  16. Min JH, Baek SY, Lee JS, Kim HK. 2011. Changes of yeasts and bacterial flora during the storage of Korean traditional Makgeolli. Kor J Mycol 39:151-153 https://doi.org/10.4489/KJM.2010.39.2.151
  17. MSU Ribosomal Database Project. http://rdp.cme.msu.edu. 2010. 9.3 방문
  18. Park CS, Lee TS. 2002. Quality characteristics of Takju prepared by wheat flour Nuruks. Korean J Food Sci Technol 34: 296-320
  19. Seo MY, Lee JK, Ahn BH, Cha SK. 2005. The chanages of microflora during the fermentation of Takju and Yakju. Korean J Food Sci Technol 37:61-66
  20. So MH, Lee YS, Noh WS. 1999. Changes in microorganisms and main components during Takju grewing by a modified Nuruk. Korean J Food & Nutr 12:226-232
  21. Sung CK. 1998. Development of natural food preservatives from agricultura products. Ph.D. Thesis, Chungnam National University. Daejeon. Korea
  22. Technical Service Institute of National Tax Service. 1997. Manufacturing Guideline of Alcoholic Beverages. pp.83-176

Cited by

  1. Antimicrobal Activity of Sutellaria baicalensis·Coptidis rhizoma Extract on the Preservation of Makgeolli vol.25, pp.4, 2012, https://doi.org/10.9799/ksfan.2012.25.4.974
  2. Characterization of Lactobacillus brevis JBE 30 as a Starter for the Brewing of Traditional Liquor vol.50, pp.3, 2014, https://doi.org/10.7845/kjm.2014.4065
  3. 알코올 내성 젖산균 Pediococcus acidilactici K3와 S1의 분리 및 생리적 특성 vol.41, pp.4, 2013, https://doi.org/10.4014/kjmb.1308.08001
  4. Fermentation and Quality Evaluation of makgeolli, Korean Rice Wine Supplemented with Alcohol-tolerant Pediococcus acidilactici K3 vol.42, pp.4, 2012, https://doi.org/10.4014/kjmb.1409.09002