Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
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Effect of Microalgal Extracts of Tetraselmis suecica against UVB-Induced Photoaging in Human Skin Fibroblasts

  • Jo, Wol Soon (Department of Research center, Dong Nam Institute of Radiological & Medical Sciences) ;
  • Yang, Kwang Mo (Department of Research center, Dong Nam Institute of Radiological & Medical Sciences) ;
  • Park, Hee Sung (Department of Research center, Dong Nam Institute of Radiological & Medical Sciences) ;
  • Kim, Gi Yong (Department of Microbiology, College of Medicine, Dong-A University) ;
  • Nam, Byung Hyouk (Test and Certification Team, Marine Bio-industry Development Center) ;
  • Jeong, Min Ho (Department of Microbiology, College of Medicine, Dong-A University) ;
  • Choi, Yoo Jin (Department of Research center, Dong Nam Institute of Radiological & Medical Sciences)
  • Received : 2012.08.29
  • Accepted : 2012.11.26
  • Published : 2012.12.31
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Abstract

Exposure of cells to ultraviolet B (UVB) radiation can induce production of free radicals and reactive oxygen species (ROS), which damage cellular components. In addition, these agents can stimulate the expression of matrix metalloproteinase (MMP) and decrease collagen synthesis in human skin cells. In this study, we examined the anti-photoaging effects of extracts of Tetraselmis suecica (W-TS). W-TS showed the strongest scavenging activity against 2,2-difenyl-1-picrylhydrazyl (DPPH) and peroxyl radicals, followed by superoxide anions from the xanthine/xanthine oxidase system. We observed that the levels of both intracellular ROS and lipid peroxidation significantly increased in UVB-irradiated human skin fibroblast cells. Furthermore, the activities of enzymatic antioxidants (e.g., superoxide dismutase) and the levels of non-enzymatic antioxidants (e.g., glutathione) significantly decreased in cells. However, W-TS pretreatment, at the maximum tested concentration, significantly decreased intracellular ROS and malondialdehyde (MDA) levels, and increased superoxide dismutase and glutathione levels in the cells. At this same concentration, W-TS did not show cytotoxicity. Type 1 procollagen and MMP-1 released were quantified using RT-PCR techniques. The results showed that W-TS protected type 1 procollagen against UVB-induced depletion in fibroblast cells in a dose-dependent manner via inhibition of UVB-induced MMP-1. Taken together, the results of the study suggest that W-TS effectively inhibits UVB-induced photoaging in skin fibroblasts by its strong anti-oxidant ability.

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References

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