Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
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The Chloroform Fraction of Carpinus tschonoskii Leaves Inhibits the Production of Inflammatory Mediators in HaCaT Keratinocytes and RAW264.7 Macrophages

  • Kang, Gyeoung-Jin (Department of Pharmacology, School of Medicine, Jeju National University) ;
  • Kang, Na-Jin (Department of Biomedicine & Drug Development, Jeju National University) ;
  • Han, Sang-Chul (Department of Pharmacology, School of Medicine, Jeju National University) ;
  • Koo, Dong-Hwan (Department of Biomedicine & Drug Development, Jeju National University) ;
  • Kang, Hee-Kyoung (Department of Pharmacology, School of Medicine, Jeju National University) ;
  • Yoo, Byoung-Sam (Cosmetic R&D Center, COSMAX Inc.) ;
  • Yoo, Eun-Sook (Department of Pharmacology, School of Medicine, Jeju National University)
  • Received : 2012.09.18
  • Accepted : 2012.12.14
  • Published : 2012.12.31
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Abstract

Inflammation is the immune system's response to infection and injury-related disorders, and is related to pro-inflammatory factors (NO, $PGE_2$, cytokines, etc.) produced by inflammatory cells. Atopic dermatitis (AD) is a representative inflammatory skin disease that is characterized by increasing serum levels of inflammatory chemokines, including macrophage-derived chemokine (MDC). Carpinus tschonoskii is a member of the genus Carpinus. We investigated the anti-inflammatory activity of C. tschonoskii by studying the effects of various solvent fractions prepared from its leaves on inflammatory mediators in HaCaT and RAW264.7 cells. We found that the chloroform fraction of C. tschonoskii inhibited MDC at both the protein and mRNA levels in HaCaT cells, acting via the inhibition of STAT1 in the IFN-${\gamma}$ signaling pathway. In addition, the chloroform fraction significantly suppressed the expression of inflammatory factors induced by lipopolysaccharide stimulation, except COX-2 and TNF-${\alpha}$. These results suggest that the chloroform fraction of C. tschonoskii leaves may include a component with potential anti-inflammatory activity.

Keywords

References

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