Journal of the Korean Society of Hazard Mitigation (한국방재학회 논문집)

Korean Society of Hazard Mitigation (한국방재학회)
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A Research for Pathological Analysis of Hydrogen Fluoride(HF) Toxicity

불화수소(HF)의 독성에 관한 병리학적 분석 연구

  • Received : 2012.10.19
  • Accepted : 2012.11.05
  • Published : 2012.12.31
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Abstract

Polymer materials are well-used for Modern building constructions. Combustion gas of construction material has different components between natural and polymer. Recently research shows, Apart from combustion gas of natural construction material contain CO. $CO_2$ species. Also polymer combustion gas include HF, HCN, HBr, HCl, NO, $NO_2$, and $SO_2$ additionally. It analyzed that species product from Polymer compounds. These species can act as major disturbance factors in the evacuation from the building's fire. Because, polymer will increase the proportion in building materials. In this research, effects from HF gas are analyzed by pathological method. Histopathologic examination, DNA toxicity evaluation experiments were conducted for mouse exposed by HF standard gas. Exposing HF gas were caused extensive hemorrhage and necrosis in the liver and lung from experimental animals. and blood and DNA damage were analyzed quantitatively by comet assay.

현대의 건축 재료는 각종 고분자 화합물의 비중이 커지고 있어 건축 재료 연소 시에 발생하는 가스의 성분비가 천연재료를 이용하던 과거의 건축물과 차이를 보인다. 실제로 근래의 건축재료 연소가스의 정성분석결과를 살펴보면, 천연재료의 연소가스에 포함되어있는 CO, $CO_2$외에도 HF, HCN, HBr, HCl, NO, $NO_2$, $SO_2$등의 화학종이 포함되어 있음을 알 수 있다. 이러한 화학종들은 고분자 화합물에서 발생하는 것으로 분석되며, 건축 재료에서 그 비중이 높아지는 만큼 향후 이러한 화학종들이 건축물 화재에서 대피의 주요 방해요인으로 작용할 수 있을 것으로 예상된다. 이에 본 연구에서는 연소가스중 HF가 실험동물의 생체에 미치는 영향을 병리학적으로 분석하기 위하여, HF의 표준가스를 실험동물에 노출시켜 조직 병리 검사, 유전 독성 평가실험을 실시하였다. 이를 통하여 HF에 노출된 실험동물은 폐와 간에서의 광범위한 출혈과 괴사가 발생함을 확인하였으며, 이로 인한 혈액과 장기별 DNA손상은 Comet assay를 이용하여 정량적으로 분석하였다.

Keywords

Acknowledgement

Supported by : 한국건설교통기술평가원

References

  1. ASTM E1678-02 (2002) Standard Test Method for Measuring Smoke Toxicity for Use in Fire Hazard Analysis, ASTM International, W. Conshohocken, PA,
  2. Cho, N.-W., Jeon, J.-P., Kim, H.-Y. and Min, B.-Y. (2006) Study of Combustibility Test of Sandwich Panel, Journal of the Architectural Institute of Korea, No. 26, Vol. 1, pp. 369-372.
  3. Cho, N.-W., Lee, J.-C. and Rie, D.-H. (2011) A Comparative Study on Toxic Gas Index and Stop Time of Mouse Activity, Journal of Korean Institute of Fire Science & Engineering, No, 25, Vol. 4, pp. 35-41.
  4. EU (2001) "Directive 67/548/EEC, Vol. 3"
  5. ISO 13344 (2004) "Determination of the Lethal Toxic Potency of Fire Effluents", International Organization for Standardization, Geneva, Switzerland.
  6. John B. Morris, Frank A. Smith (1982) "Regional deposition and absorption of inhaled hydrogen fluoride in the rat", Toxicology and applied pharmacology, Vol. 62, pp. 81-89. https://doi.org/10.1016/0041-008X(82)90104-1
  7. Korea Standards Association (1998) KS F 2271 : Fire Retardant Testing Method of Interior Finishes and Structures.
  8. KOSHA (2011) MSDS(Material safety data sheet).
  9. Narendra. P. Singh, Michael T. Mccoy (1988) "A simple technique for quantitation of low levels of DNA damage in individual cells" experimental Cell Research, March 1988 Vol. 175 pp. 184-191. https://doi.org/10.1016/0014-4827(88)90265-0
  10. NFPA (2003) "Fire protection handbook", pp. 6-17 (Twentieth Edition)
  11. Olivier arbier, Laura Arreola-Mendoza, Lus Maria Del Razo (2010) "Molecular mechanisms of fluoride toxicity", Chemico Biological Interactions.
  12. Ostling, O., Johanson, K.J (1984) "Microelectrophoretic study of radiation-induced DNA damages in individual mammalian cells", Biochemical and Biophysical Research Communications, 30 August 1984 Vol. 123, pp. 291-298. https://doi.org/10.1016/0006-291X(84)90411-X
  13. Rie, D.-H., Cho, N.-W. and Choi, S.-Y. (2012) A Study on Toxicity Bio-markers of a Mouse using Combustion Gas SO2 generated from Fire, Journal of the Korea Safety Management & Science, No. 14, Vol. 1. https://doi.org/10.12812/ksms.2012.14.1.043
  14. SFPE (2005) "The SFPE Handbook of Fire Protection Engineering", Section Three. HAZARD CALCULATIONS, pp. 3-85 - 3-86 (3rd edition).
  15. SOLAS (2002) "FTP Code : International Code for Application of Fire Test Procedures".
  16. Xiaochen Qiu, Hengyu Li, Hongtai Tang, Yichao Jin, Wuquan Li, Yusun, PingFeng, Xuejun Sun, Zhaofan Xia (2011) "Hydrogen Inhalation ameliorates lipopolysaccharide-induced acute lung injury in mice" international Immunopharmacology.