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Cultivation of Paecilomyces tenuipes using Mini-kit, small culture container

소규모 재배상을 이용한 생동충하초 재배

  • 남성희 (농촌진흥청 국립농업과학원 농업생물부) ;
  • 이광길 (농촌진흥청 국립농업과학원 농업생물부) ;
  • 여주홍 (농촌진흥청 국립농업과학원 농업생물부) ;
  • 이희삼 (농촌진흥청 국립농업과학원 농업생물부) ;
  • 황재삼 (농촌진흥청 국립농업과학원 농업생물부) ;
  • 최영철 (농촌진흥청 국립농업과학원 농업생물부) ;
  • 박관호 (농촌진흥청 국립농업과학원 농업생물부)
  • Received : 2012.04.18
  • Accepted : 2012.10.15
  • Published : 2012.12.30

Abstract

Cordyceps and its allies fungi has been described as a secret medicine that gives eternal youth and a long life. Some species of Cordyceps are sources of biochemicals, such ascordycepin, with interesting biological and pharmacological properties. Hence, it has been studied to uncover its pharmacological effect. We attempted to study the formation of fruiting bodies and to develop means of mass production Korean isolate of Paecilomyces tenuipes has been inoculated into silkworms, where it reproduced using culture container, mini-kit successfully. Culture container, mini-kit is composed of a cylinder-shaped body and lid. The container is made of translucent polyethylene terephthalate. The size of the container is $82{\times}75mm$, reduced by 10 times as compared with the conventional culture kit. The mini kit has many advantages - high culture amount, ability of maintaining optimal humidity, parasite-free cultivation and high-end appearance. With the kit, the optimal cultivation condition is under $22^{\circ}C$, culture period of 53 days. And synnemata of P. tenuipes could be kept fresh for 14 days at the temperature of under $10^{\circ}C$. Therefore, the Min-kit can be used in both ways as a culture container and a packing kit for end-user customers.

신선한 생동충하초를 소규모 용기에서 재배, 유통할 수 있는 기술개발에 대한 수요가 급증함에 따라, 소규모 재배상인 미니키트를 선발하고 장기간 보존 및 유통기간을 연장할 수 있는 최적 재배 조건을 구명하였다. 미니키트는 몸체가 투명한 PET, 뚜껑은 PP 재질로 구성되며 350 ml 용량으로 높이 ($H:82mm{\times}W:75mm$) 용량의 밀폐형으로 구성되며 단위 당 투입 감염 번데기 수는 15~20개체 재배가 적합하였다. 미니키트 내 동충하초 생산은 총 53일이 소요되며 키트 내에서 자실체 생장기간은 15일이었으며, 배양은 온도 $22^{\circ}C$ 하에서 생산성이 가장 우수하였다. 미니키트 동충하초의 저장력은 냉장처리 14일까지 신선도를 90%로 유지하였다. 현재까지 농가 및 소비자로부터 동충하초의 소량확보 및 생동충하초의 유통에 관한 요구가 늘어나고 있는 추세이다. 이러한 현실에 맞게 미니키트 동충하초는 생초로 유통하는 것이 주요 특징으로 밀폐용기를 적용함으로써 오염방지 및 위생적 관리가 가능하며 동결건조 공정이 생략됨으로써 공정을 줄이는 장점이 있다. 따라서 미니키트는 일반소비자로부터 생동충하초의 확보가 용이하게 되어, 식품소재로 널리 활용할 수 있을 것으로 예상된다.

Keywords

References

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