Journal of Acupuncture Research

Korean Acupuncture & Moxibustion Medicine Society (대한침구의학회)
권호 표지

Effects of Astragalus Membranaceus, Innamomum Cassia, Phellodendron Amurensis(BHH10) on MC3T3-E1 Cells Proliferation, Differntiation and Bone Mineralized Formation

MC3T3-E1 세포주에서 황기.계지.황백 처방(BHH10)의 골형성 촉진 효능 연구

  • Lee, Mi Lim (Dept. of Cancer Preventive Material Development, College of Korean Medicine, Kyung Hee University) ;
  • Huh, Jeong Eun (Oriental Medicine Research Center for Bone & Joint Disease, Kyung-Hee University) ;
  • Nam, Dong Woo (Department of Acupuncture & Moxibustion Medicine, College of Korean Medicine, Kyung-Hee University) ;
  • Seon, Jong In (Department of Acupuncture & Moxibustion Medicine, College of Korean Medicine, Kyung-Hee University) ;
  • Kang, Jung Won (Department of Acupuncture & Moxibustion Medicine, College of Korean Medicine, Kyung-Hee University) ;
  • Kim, Sung Hoon (Dept. of Cancer Preventive Material Development, College of Korean Medicine, Kyung Hee University) ;
  • Choi, Do Young (Department of Acupuncture & Moxibustion Medicine, College of Korean Medicine, Kyung-Hee University) ;
  • Lee, Jae Dong (Department of Acupuncture & Moxibustion Medicine, College of Korean Medicine, Kyung-Hee University)
  • 이미림 (경희대학교 한의과대학 암예방소재개발센터) ;
  • 허정은 (경희대학교 골관절질환 한방연구센터) ;
  • 남동우 (경희대학교 한의과대학 침구의학교실) ;
  • 선종인 (경희대학교 한의과대학 침구의학교실) ;
  • 강중원 (경희대학교 한의과대학 침구의학교실) ;
  • 김성훈 (경희대학교 한의과대학 암예방소재개발센터) ;
  • 최도영 (경희대학교 한의과대학 침구의학교실) ;
  • 이재동 (경희대학교 한의과대학 침구의학교실)
  • Received : 2012.09.10
  • Accepted : 2012.11.28
  • Published : 2012.12.20
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Abstract

Objectives : BHH10 is traditional medicine herb used for enhancing body resistance against various diseases. The aim of this study was to identify BHH10 extract induces osteogenic activity in human osteoblast-like MC3T3-E1 cells. Methods : MC3T3-E1, pre-osteoblast cell line, were treated with BHH10 of various concentrations($0.1{\mu}g/mL$, $1{\mu}g/mL$, $10{\mu}g/mL$). And then, the effect of BHH10 on osteoblast differentiation was examined by alkaline phosphatase(ALP) activity, von Kossa staining and RT-PCR for osteoblast differentiation markers such as osteocalcin(OCN), osteopontin(OPN). Results : BHH10 had dose-dependent effect on the viability of osteoblastic cells, and dose-dependently increased alkaline phosphatase(ALP) activity. BHH10 markedly increased mRNA expression for OCN, OPN in MC3T3-E1 cells. Also, BHH10 significantly induced mineralization in the culture of MC3T3-E1 cells. Conclusions : In conclusion, these results propose that BHH10 can play an important role in osteoblastic bone formation, osteogenesis, and may possibly lead to the development of bone-forming drugs.

Keywords

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