DOI QR코드

DOI QR Code

Molecular Identification of Endophytic Fungi Isolated from Needle Leaves of Conifers in Bohyeon Mountain, Korea

  • Yoo, Jae-Joon (Department of Biology Education, Korea National University of Education) ;
  • Eom, Ahn-Heum (Department of Biology Education, Korea National University of Education)
  • Received : 2012.11.13
  • Accepted : 2012.12.07
  • Published : 2012.12.31

Abstract

Fungal endophytes are microfungi that live in plants without causing apparent symptoms of infection. This study was conducted to identify endophytic fungi isolated from leaves of coniferous trees in Bohyeon Mountain of Korea. We collected leaves of two species of coniferous trees, Pinus densiflora and Pinus koraiensis, from 11 sites in the study area. A total 58 isolates were obtained and identified using molecular and morphological characteristics. Four species of endophytic fungi were isolated from P. densiflora: Lophodermium conigenum, Leotiomycetes sp., Septoria pini-thunbergii, and Polyporales sp., while two fungal species were isolated from P. koraiensis: Eurotiomycetes sp. and Rhytismataceae sp. The most frequently isolated species were L. conigenum and S. pini-thunbergii.

Keywords

References

  1. Petrini O. Fungal endophytes of tree leaves. In: Adrews JH, Hirano SS, editors. Microbial ecology of leaves. New York: Springer-Verlag; 1991. p. 179-97.
  2. Carroll GC, Carroll FE. Studies on the incidence of coniferous needle endophytes in the Pacific Northwest. Can J Bot 1978;56:3034-43. https://doi.org/10.1139/b78-367
  3. Stone JK, Bacon CW, White JF Jr. An overview of endophytic microbes: endophytism defined. In: Bacon CW, White JF Jr, editors. Microbial endophytes. New York: Marcel Dekker; 2000. p. 3-30.
  4. Kriel WM, Swart WJ, Crous PW. Foliar endophytes and their interactions with host plants, with specific reference to the gymnospermae. Adv Bot Res 2000;33:1-34.
  5. Muller MM, Valjakka R, Suokko A, Hantula J. Diversity of endophytic fungi of single Norway spruce needles and their role as pioneer decomposers. Mol Ecol 2001;10:1801-10. https://doi.org/10.1046/j.1365-294X.2001.01304.x
  6. Arnold AE, Henk DA, Eells RL, Lutzoni F, Vilgalys R. Diversity and phylogenetic affinities of foliar fungal endophytes in loblolly pine inferred by culturing and environmental PCR. Mycologia 2007;99:185-206. https://doi.org/10.3852/mycologia.99.2.185
  7. Strobel G, Daisy B, Castillo U, Harper J. Natural products from endophytic microorganisms. J Nat Prod 2004;67:257-68. https://doi.org/10.1021/np030397v
  8. Gao XX, Zhou H, Xu DY, Yu CH, Chen YQ, Qu LH. High diversity of endophytic fungi from the pharmaceutical plant, Heterosmilax japonica Kunth revealed by cultivationindependent approach. FEMS Microbiol Lett 2005;249:255-66. https://doi.org/10.1016/j.femsle.2005.06.017
  9. Kil YJ, Eo JK, Eom AH. Molecular identification and diversity of endophytic fungi isolated from Pinus densiflora in Boeun, Korea. Kor J Mycol 2009;37:130-3. https://doi.org/10.4489/KJM.2009.37.2.130
  10. Kim CK, Eo JK, Eom AH. Diversity of foliar endophytic fungi isolated from Lindera obtusiloba in Korea. Kor J Mycol 2012;40:136-40. https://doi.org/10.4489/KJM.2012.40.3.136
  11. Edelstein L, Hadar Y, Chet I, Henis Y, Segel LA. A model for fungal colony growth applied to Sclerotium rolfsii. J Gen Microb 1983;129:1873-81.
  12. Photita W, Taylor PW, Ford R, Hyde KD, Lumyong S. Morphological and molecular characterization of Colletotrichum species from herbaceous plants in Thailand. Fungal Divers 2005;18:117-33.
  13. White TJ, Bruns T, Lee S, Taylor J. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ, editors. PCR protocols: a guide to methods and applications. New York: Academic Press; 1990. p. 315-22.
  14. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 2011;28:2731-9. https://doi.org/10.1093/molbev/msr121
  15. Nei M, Kumar S. Molecular evolution and phylogenetics. New York: Oxford University Press; 2000.
  16. Stone JK. Foliar endophytes of Pseudotsuga menziesii (Mirb.) Franco. Cytology and physiology of the host-endophyte relationship [dissertation]. Eugene: University of Oregon; 1986.
  17. Petrini O. Taxonomy of endophytic fungi of aerial plant tissues. In: Fokkema NJ, van den Heuvel J, editors. Microbiology of th Phyllospere. Cambridge: Cambridge University Press; 1986. p. 175-87.
  18. Arnold A. Neotropical fungal endophytes: diversity and ecology [dissertation]. Tucson: University of Arizona; 2002.
  19. Lacap DC, Hyde KD, Liew EC. An evaluation of the fungal 'morphotype'concept based on ribosomal DNA sequences. Fungal Divers 2003;12:53-66.