Mycobiology

The Korean Society of Mycology (한국균학회)
권호 표지
DOI QR코드

DOI QR Code

Application of Rhizobacteria for Plant Growth Promotion Effect and Biocontrol of Anthracnose Caused by Colletotrichum acutatum on Pepper

  • Lamsal, Kabir (Department of Applied Plant Sciences, Kangwon National University) ;
  • Kim, Sang Woo (Department of Applied Plant Sciences, Kangwon National University) ;
  • Kim, Yun Seok (Department of Applied Plant Sciences, Kangwon National University) ;
  • Lee, Youn Su (Department of Applied Plant Sciences, Kangwon National University)
  • Received : 2012.10.13
  • Accepted : 2012.11.14
  • Published : 2012.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

In vitro and greenhouse screening of seven rhizobacterial isolates, AB05, AB10, AB11, AB12, AB14, AB15 and AB17, was conducted to investigate the plant growth promoting activities and inhibition against anthracnose caused by Colletotrichum acutatum in pepper. According to identification based on 16S rDNA sequencing, the majority of the isolates are members of Bacillus and a single isolate belongs to the genus Paenibacillus. All seven bacterial isolates were capable of inhibiting C. acutatum to various degrees. The results primarily showed that antibiotic substances produced by the selected bacteria were effective and resulted in strong antifungal activity against the fungi. However, isolate AB15 was the most effective bacterial strain, with the potential to suppress more than 50% mycelial growth of C. acutatum in vitro. Moreover, antibiotics from Paenibacillus polymyxa (AB15) and volatile compounds from Bacillus subtilis (AB14) exerted efficient antagonistic activity against the pathogens in a dual culture assay. In vivo suppression activity of selected bacteria was also analyzed in a greenhouse with the reference to their prominent in vitro antagonism efficacy. Induced systemic resistance in pepper against C. acutatum was also observed under greenhouse conditions. Where, isolate AB15 was found to be the most effective bacterial strain at suppressing pepper anthracnose under greenhouse conditions. Moreover, four isolates, AB10, AB12, AB15, and AB17, were identified as the most effective growth promoting bacteria under greenhouse conditions, with AB17 inducing the greatest enhancement of pepper growth.

Keywords

References

  1. Oh BJ, Kim KD, Kim YS. Effect of cuticular wax layers of green and red pepper fruits on infection by Colletotrichum gloeosporioides. J Phytopathol 1999;147:547-52. https://doi.org/10.1111/j.1439-0434.1999.tb03863.x
  2. Kim WG, Cho EK, Lee EJ. Two strains of Colletotrichum gloeosporioides Penz. causing anthracnose on pepper fruits. Korean J Plant Pathol 1986;2:107-13.
  3. Freeman S, Katan T, Shabi E. Characterization of Colletotrichum species responsible for anthracnose diseases of various fruits. Plant Dis 1998;82:596-605. https://doi.org/10.1094/PDIS.1998.82.6.596
  4. Lewis Ivey ML, Nava-Diaz C, Miller SA. Identification and management of Colletotrichum acutatum on immature bell peppers. Plant Dis 2004;88:1198-204. https://doi.org/10.1094/PDIS.2004.88.11.1198
  5. Peres NA, Timmer LW, Adaskaveg JE, Correll JC. Lifestyles of Colletotrichum acutatum. Plant Dis 2005;89:784-96. https://doi.org/10.1094/PD-89-0784
  6. Kim JT, Park SY, Choi W, Lee YH, Kim HT. Characterization of Colletotrichum isolates causing anthracnose of pepper in Korea. Plant Pathol J 2008;24:17-23. https://doi.org/10.5423/PPJ.2008.24.1.017
  7. Strange RN. Plant disease control: towards environmentally acceptable methods. New York: Chapman and Hall Publishing; 1993. p. 354.
  8. Shanmugam V, Kanoujia N. Biological management of vascular wilt of tomato caused by Fusarium oxysporum f. sp. lycospersici by plant plant growth-promoting rhizobacterial mixture. Biol Control 2011;57:85-93. https://doi.org/10.1016/j.biocontrol.2011.02.001
  9. Jetiyanon K, Kloepper JW. Mixtures of plant growthpromoting rhizobacteria for induction of systemic resistance against multiple plant diseases. Biol Control 2002;24:285-91. https://doi.org/10.1016/S1049-9644(02)00022-1
  10. van Loon LC, Bakker PA, Pieterse CM. Systemic resistance induced by rhizosphere bacteria. Annu Rev Phytopathol 1998;36:453-83. https://doi.org/10.1146/annurev.phyto.36.1.453
  11. Lugtenberg B, Kamilova F. Plant growth-promoting rhizobacteria. Annu Rev Microbiol 2009;63:541-56. https://doi.org/10.1146/annurev.micro.62.081307.162918
  12. Raaijmakers JM, Vlami M, de Souza JT. Antibiotic production by bacterial biocontrol agents. Antonie Van Leeuwenhoek. 2002; 81:537-47. https://doi.org/10.1023/A:1020501420831
  13. Pal KK, Gardener BM. Biological control of plant pathogens. The Plant Health Instructor [Internet]. St. Paul: American Phytopathological Society; 2006 [cited 2012 Dec 1]. Available from: http://dx.doi.org/10.1094/PHI-A-2006-1117-02.
  14. Ulloa M, Hanlin RT. Illustrated dictionary of mycology. St. Paul: APS Press; 2000.
  15. Bharathi R, Vivekananthan R, Harish S, Ramanathan A, Samiyappan R. Rhizobacteria-based bio-formulations for the management of fruit rot infection in chillies. Crop Prot 2004;23:835-43. https://doi.org/10.1016/j.cropro.2004.01.007
  16. Weisburg WG, Barns SM, Pelletier DA, Lane DJ. 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 1991;173:697-703. https://doi.org/10.1128/jb.173.2.697-703.1991
  17. Reysenbach AL, Giver LJ, Wickham GS, Pace NR. Differential amplification of rRNA genes by polymerase chain reaction. Appl Environ Microbiol 1992;58:3417-8.
  18. O'Brien RG, O'Hare PJ, Glass RJ. Cultural practices in the control of bean root rot. Aust J Exp Agric 1991;31:551-5. https://doi.org/10.1071/EA9910551
  19. Villajuan-Abgona R, Kageyama K, Hyakumachi M. Biocontrol of Rhizoctonia damping-off of cucumber by nonpathogenic binucleate Rhizoctonia. Eur J Plant Pathol 1996;102:227-35. https://doi.org/10.1007/BF01877961
  20. Skerlavaj B, Benincasa M, Risso A, Zanetti M, Gennaro R. SMAP-29: a potent antibacterial and antifungal peptide from sheep leukocytes. FEMS Lett 1999;463:58-62.
  21. Williams GE, Asher MJ. Selection of rhizobacteria for the control of Pythium ultimum and Aphanomyces cochlioides on sugar-beet seedlings. Crop Prot 1996;15:479-86. https://doi.org/10.1016/0261-2194(96)00014-2
  22. Montealegre JR, Reyes R, Perez LM, Herrera R, Silva P, Besoain X. Selection of bioantagonistic bacteria to be used in biological control of Rhizoctonia solani in tomato. Electron J Biotechnol 2003;6:115-27.
  23. Sariah M. Potential of Bacillus spp. as a biocontrol agent for anthracnose fruit rot of chilli. Malays Appl Biol 1994;23:53-60.
  24. Dijksterhuis J, Sanders M, Gorris LG, Smid EJ. Antibiosis plays a role in the context of direct interaction during antagonism of Paenibacillus polymyxa towards Fusarium oxysporum. J Appl Microbiol 1999;86:13-21. https://doi.org/10.1046/j.1365-2672.1999.t01-1-00600.x
  25. Mavingui P, Heulin T. In vitro chitinase and antifungal activity of a soil, rhizosphere and rhizoplane population of Bacillus polymyxa. Soil Biol Biochem 1994;26:801-3. https://doi.org/10.1016/0038-0717(94)90277-1
  26. Compant S, Duffy B, Nowak J, Clement C, Barka EA. Use of plant growth-promoting bacteria for biocontrol of plant diseases: principles, mechanisms of action, and future prospects. Appl Environ Microbiol 2005;71:4951-9. https://doi.org/10.1128/AEM.71.9.4951-4959.2005
  27. Choudhary DK, Johri BN. Interactions of Bacillus spp. and plants with special reference to induced systemic resistance (ISR). Microbiol Res 2009;164:493-513. https://doi.org/10.1016/j.micres.2008.08.007
  28. Kloepper JW, Ryu CM, Zhang S. Induced systemic resistance and promotion of plant growth by Bacillus spp. Phytopathology 2004;94:1259-66. https://doi.org/10.1094/PHYTO.2004.94.11.1259
  29. Mia MA, Shamsuddin ZH, Wahab Z, Marziah M. Highyielding and quality banana production through plant growthpromoting rhizobacterial (PGPR) inoculation. Fruits 2005; 60:179-85. https://doi.org/10.1051/fruits:2005024