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The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Inhibition of Adipogenesis in 3T3-L1 Adipocytes with Ethanol Extracts of Saururus chinensis

삼백초 추출물의 3T3-L1 세포에 대한 지방축적 억제효과

  • 신옥수 (중앙대학교 의과대학 병리학교실) ;
  • 신연호 (중앙대학교 의과대학 병리학교실) ;
  • 이강혁 (바이오랜드 생명공학연구소) ;
  • 김건용 (바이오랜드 생명공학연구소) ;
  • 김기호 (바이오랜드 생명공학연구소) ;
  • 박정극 (동국대학교 의생명공학과) ;
  • 안재일 (충북테크노파크 바이오센터) ;
  • 송계용 (중앙대학교 의과대학 병리학교실)
  • Received : 2012.09.11
  • Accepted : 2012.11.16
  • Published : 2012.12.31
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Abstract

Obesity increases the risk of many adult diseases, such as atherosclerosis, diabetes, hypertension, ischemic heart disease and breast cancer. Inhibition of adipogenesis is an effective way in the anti-obesity management. Because of main components of Saururus chinensis is flavonoid, it has been showed some improvement by its antioxidant effects on the atherosclerosis, heart disease and diabetic hyperlipidemia. But mechanism of anti-obesity effect of S. chinensis is not clear. We investigated the effects of ethanol extracts of S. chinensis on adipogenesis in the 3T3-L1 pre-adipocyte. The 3T3-L1 cell line is commonly used to study adipogenesis in vitro. In this study, ethanol extracts of S. chinensis significantly decrease the lipid accumulation in the 3T3-L1 cells proved by measuring triglyceride contents and Oil red O staining. The proposed mechanism of inhibition of adipogenesis in the 3T3-L1 cells with ethanol extracts of S. chinensis is down-regulation of transcriptional factors and adipocyte-specific genes such CCAAT/enhancer binding protein ${\alpha}$ ($C/EBP{\alpha}$) and Peroxisome proliferator activated receptor ${\gamma}$ ($PPAR{\gamma}$) in concentration dependent pattern. These results suggest that ethanol extracts of S. chinensis inhibits adipognesis in the 3T3-L1 cells and can be used as a safe and efficient natural substance to manage anti-obesity.

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References

  1. Lee, S. R., C. H. Park, E. Y. Kuan, Y. Q. Lu, H. Kim, K. C. Kim, H. U. Son, H. J. Lee, J. C. Heo, and S. H. Lee (2011) Comparision of anti-adipogenesis activity by several grape extracts. Korean J. Food Preserv. 18: 119-123. https://doi.org/10.11002/kjfp.2011.18.1.119
  2. Kim, D. J., J. H. Jung, S. G. Kim, H. K. Lee, S. K. Lee, H. D. Hong, B. Y. Lee, and O. H. Lee (2011) Antioxidants and antiobesity activities of hot water and ethanolic extracts from Cheonnyuncho. Korean J. Food Preserv. 18: 366-373. https://doi.org/10.11002/kjfp.2011.18.3.366
  3. Bradford, B. L. and M. S. Bruce (2000) Towards a molecular understanding of adaptive thermogenesis. Nature 404: 652-660. https://doi.org/10.1038/35007527
  4. Rayalam, S., M. A. Della-Fera, and C. A. Baile (2008) Phytochemicals and regulation of the adipocyte life cycle. J. Nutritional Biochemistry 19: 717-726. https://doi.org/10.1016/j.jnutbio.2007.12.007
  5. Dave, S., N. J. Kaur, R. Nanduri, H. K. Dkhar, A. Kumar, and P. Gupta (2012) Inhibition of adipogenesis and induction of apoptosis and lipolysis by stem bromelanin in 3T3-L1 adipocytes. PLos One 7: e30831. https://doi.org/10.1371/journal.pone.0030831
  6. Park, M. Y., D. W. Seo, J. Y. Lee, M. K. Sung, Y. M. Lee, H. H. Jang, H. Y. Choi, J. H. Kim, and D. S. Park (2011) Effects of Panicum miliaceum L. extract on adipogenic transcription factors and fatty acid accumulation and 3T3-L1 adipocytes. Nutr. Res. Pract. 5: 192-197. https://doi.org/10.4162/nrp.2011.5.3.192
  7. Kim, T. J. (1996) Korean Esource Plants. pp. 66-67. Seoul National University Press, Seoul. Korea.
  8. Seo, C. S., W. H. Lee, H. W. Chung, E. J. Chang, S. H. Lee, Y. Jahng, B. Y. Hwang, J. K. Son, S. B. Han, and Y. S. Kim (2009) Manassantin A and B from Saururus chinensis inhibiting cellular melanin production. Phytoher. Res. 23: 1531-1536. https://doi.org/10.1002/ptr.2791
  9. Choi, H. N., Y. H. Park, J. H. Kim, M. J. Kang, S. M. Jeong, H. H. Kim, and J. I. Kim (2011) Renoprotective and antioxidant effects of Saururus chinensis baill in rats fad a high-fructose diet. Nutr. Res. Pract. 5: 365-369. https://doi.org/10.4162/nrp.2011.5.4.365
  10. Kim, H. S. (2006) The Effects of Saururus chinensis baill extract administration on the blood glucose, electrolyte and lipid metabolism in stz-induced hyperglycemic rats. J. Life Science 16: 911-918. https://doi.org/10.5352/JLS.2006.16.6.911
  11. Hwang, J. Y., J. Zhang, M. J. Kang, S. K. Lee, H. A. Kim, and J. I. Kim (2007) Hypoglycemic and hypolipidemic effects of Sarurus chinensis baill in streptozotocin-induced diabetic rats. Nutr. Res. Pract. 2: 100-104.
  12. Yoon, B. R., B. J. Cho, H. K. Lee, D. J. Kim, S. K. Rhee, H. D. Hong, K. T. Kim, C. W. Cho, H. S. Choi, B. Y. Lee, and O. H. Lee (2012) Antioxidant and anti-adipogenic effects of ethanolic extracts from tartary and common buckwheats. Korean J. Food Preserv. 19: 123-130. https://doi.org/10.11002/kjfp.2012.19.1.123
  13. Lee, M. S., C. T. Kim, I. H. Kim, and Y. H. Kim (2011) Effects of capsaicin on lipid catabolism in 3T3-L1 adipocytes. Phytother. Res. 25, 935-939. https://doi.org/10.1002/ptr.3339
  14. Desai, A., T. Vyas, and M. Amiji (2008) Cytotoxicity and apoptosis enhancement in brain tumor cells upon coadministration of paclitaxel and ceramide in nanoemulsion formulations. J. Pharma Sci. 97: 2745-2756. https://doi.org/10.1002/jps.21182
  15. Spiegelman, B. M. and S. Filer (1996) Adipogenesis and obesity: rounding out the big picture. Cell. 87: 377-389. https://doi.org/10.1016/S0092-8674(00)81359-8
  16. Raylama S., M. A. Della-Fera, and C. A. Baile (2008) Phytochemicals and regulation of the adipocyte life cycle. J. Nutr. Biochem. 19: 717-726. https://doi.org/10.1016/j.jnutbio.2007.12.007
  17. Chin, H. S., K. J. Pack, S. H. Pack, and J. K. Kim (2009) The effects of herbal extract mixture on anti-obesity. J. Korean Soc. Food Sci. Nutr. 38: 32-38. https://doi.org/10.3746/jkfn.2009.38.1.032
  18. Kang, S. R., E. Y. Park, M. S. Park, J. Park, and Y. C. Kim (2011) Antioxidative and collagen synthetic abilities of Gardeniae fructus and Sarurus chinensis water extracts. J. Investigative Cosmetology 7: 165-171. https://doi.org/10.15810/jic.2011.7.2.010
  19. Lee, J. W., H. J. Park, J. S. Kim, E. Y. Choi, S. N. Han, E. S. Seong, C. Y. Yu, Y. S. Kwon, and M. J. Kim (2011) Isolation of flavonol glycoside related to antioxidant activity from hippophae rhamnoides leaves. Korean J. Mdeicinal Crop Sci. 19: 251-256. https://doi.org/10.7783/KJMCS.2011.19.4.251
  20. Yahagi, Tadahiro., Akihiro Daikonya, and Susumu Kitanaka (2012) Flavonol acylglycoside from flower of Albizia julibrissin and their inhibitory effects on lipid accumulation in 3T3-L1 cells. Chem. Pharm. Bull. 60: 129-136. https://doi.org/10.1248/cpb.60.129
  21. Kim, M. J., I. J. Kim, S. Y. Nam, C. H. Lee, T. Yun, and B. H. Song (2006) Effects of drying methods on contents of active components, antioxidant activity and color values of Saururus chinensis Baill. Korean J. Medicinal Crop. Sci. 14: 8-13.
  22. Choi, M. J., H. K. Jung, Y. S. Jeong, S. C. Park, and J. H. Hong (2010) Anti-allergic activities of fermented Eriobotrya japonica and Saururus chinensis extracts in 2,4-dinitrochlorobenzeneinduced BALB/c mice. J. Korean Soc. Food Sci. Nutr. 39: 1611-1618. https://doi.org/10.3746/jkfn.2010.39.11.1611
  23. Rosen, E. D. (2005) The transcriptional basis of adipocyte development. Prostaglandins Leukot Essent Fatty Acids 73: 31-34. https://doi.org/10.1016/j.plefa.2005.04.004
  24. Evans, R. M., G. D. Barish, and Y. X. Wang (2004) PPARs and the complex journey to obesity. Nat. Med. 10: 355-361. https://doi.org/10.1038/nm1025

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