Korean Journal of Soil Science and Fertilizer (한국토양비료학회지)

Korean Society of Soil Science and Fertilizer (한국토양비료학회)
권호 표지
DOI QR코드

DOI QR Code

Changes of Biological and Chemical Properties during Composting of Livestock Manure with Isolated Native Microbe

토착미생물별 가축분 퇴비화 과정중 생물화학적 특성 변화

  • Han, Hyo-Shim (Department of Bio-Environment & Chemistry, Sunchon National University) ;
  • Lee, Kyung-Dong (Department of Oriental Medicine Materials, Dongshin University)
  • 한효심 (순천대학교 생물환경학과) ;
  • 이경동 (동신대학교 한약재산업학과)
  • Received : 2012.11.30
  • Accepted : 2012.12.12
  • Published : 2012.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

In order to produce high-quality fermenting composts, bacteria strains with high activities of extracellular enzymes (cellulase, chitinase, amylase, protease and lipase) were isolated from the soils in 6 provinces of Korea, and characterized by 16S rRNA gene sequence analysis and properties. The selected 7 stains inoculated to livestock manure for 2' fermenting time, and experimental treatment divided into 3 groups, B1, B2 and B3, according to microbial activity and enzyme type. Our results showed that microbe applications (B1, B2 and B3) can increase (p<0.05) both rhizomes (17-38%) and enzyme activities (50-81%) in compost after fermenting time, respectively, compared to non-microbe treatment (control). The microbe application also decreased significantly (p<0.05) the $NH_3$ and $H_2S$ gas contents 13.4 and 27.3% compared with control, and the Propionic acid and Butyric acid gas contents 14.5 and 19.6%, respectively, as compared to the control. The microbial degradation rate (%) of pesticides and heavy metals increased significantly (p<0.05) after fermenting time, respectively, as compared to the control. Especially, microbe applications were more effective in total rhizomes yields and bioactivities than non-microbe treatment. Thus the results of this study could help in development of potential bioinoculants and composting techniques that maybe suitable for crop production, and protectable for earth environment under various conditions.

다양한 기능을 가진 토착미생물을 분리 동정 후 퇴비화에 적용하여 우수한 기능성을 가진 발효퇴비를 제조하고자 하였다. 7개의 우수 토착미생물을 주축으로 3개의 발효퇴비를 제조하여 생리활성을 조사한 결과, 대두의 발아율 증가, 섬유소, 키틴, 단백질 분해 등의 생리활성이 대조구보다 뛰어나고, 오옥신 생산량이 유의적으로 증가하였다. 퇴비중 암모니아 가스와 휘발성 지방산 함량 역시 2차 발효가 시작되는 20일까지 함량 감소가 있었고, 대두의 건물수량이 증가함을 확인하였다. 또한 식물보호제 분해시험에서도 유의적인 분해율이 있었고, 중금속 함량 역시 유의적으로 감소하였다. 따라서 토착미생물의 다 기능적 활성을 활용한 발효퇴비의 제조는 기존보다 더 향상된 발효퇴비를 농업생산 환경에 적용할 수 있을 것이다.

Keywords

References

  1. Frankenberger, W.T. and W. Bruner. 1983. Method of detection of auxin-indole-3-acetic acid in soils by high performance liquid chromatography. Soil Sci. Soc. Am. J. 47:237-241. https://doi.org/10.2136/sssaj1983.03615995004700020012x
  2. Go, W.R., J.Y. Kim, J.H. Yoo, J.H. Lee, A. Kunhikrishnan, J.M. Lee, K.H. Kim, D.H. Kim, and W.I. Kim. 2012. Monitoring of heavy metals in agricultural soils from consecutive applications of commercial liquid pig manure. Korean J. Environ. Agric. 31:217-223. https://doi.org/10.5338/KJEA.2012.31.3.217
  3. Haddaoui, E., M. Francoise, P. Glatron, and R. Chambert. 1995. Characterization of a new cell bound alpha amylase in Bacillus subtilis 168 marburg that is only immunologically related to the exocellular alpha amylase. J. Bacteriol. 177:5148-5150. https://doi.org/10.1128/jb.177.17.5148-5150.1995
  4. Ham, S.K., Y.S. Kim, and C.H. Park. 2010. The growth effects of creeping bentgrass by SCB (Slurry composting and biofilteration) liquid fertilizer application, Korean J. Turf. Sci. 24:56-61.
  5. Han, H.S., S. Woo, D.K. Kim, B.G. Heo, and K.D. Lee. 2010. Effects of composts on the growth, yield and effective components of Turmeric (Curcuma longa L.). Korean J. Environ. Agri. 29:138-145. https://doi.org/10.5338/KJEA.2010.29.2.138
  6. Hanrahan, T.J. 1989. Use of somatotrophin in livestock production: growth in pigs. In: Serjrsen, K., Eds, Use of somatotrophin in livestock production, commission of the European communities. Elsevier Scientific, UK.
  7. Hong, J.H. and K.J. Park. 2009. Composting characteristics of food waste-poultry manure mixture inoculated with effective microorganisms. J. Lives Hous. & Env. 15:59-68.
  8. James, N. 1958. Soil extraction in soil microbiology. Can. J. Microbiol. 4: 363-370. https://doi.org/10.1139/m58-038
  9. Jeong, J.Y., K.Y. Jung, and S.S. Nam. 1999. Evaluation of compost qualities with or without microbial inoculation for food waste composting. Korean J. Environ. Agri. 18:280-286.
  10. Jo, N.C., J.S. Shin, S.H. Kim, S.H. Yoon, B.S. Hwang, M.W. Jung, K.D. Lee, W.H. Kim, S. Seo, J.G. Kim, C.E. Song, and K.C. Choi. 2010. Study on summer forage crop cultivation using SCB (Slurry composting-biofilteration) liquid fertilizer on reclaimed land, J. Kor. Grassl. Forage Sci. 30:121-126. https://doi.org/10.5333/KGFS.2010.30.2.121
  11. Kim, K.J., Y.J. Yang, and J.G. Kim. 2003. Purification and characterization of chitinase from Streptomyces sp. M-20. J. Biochem. & Mol. Biol. 36:185-189. https://doi.org/10.5483/BMBRep.2003.36.2.185
  12. Kunitz, M. 1947. Crystalline soybean trypsin inhibitor. J. Gen Physiol. 30:291-297. https://doi.org/10.1085/jgp.30.4.291
  13. Kuster, E. and S.T. Williams. 1964. Selection of media for isolation of Streptomycetes. Nature. 202:928-929. https://doi.org/10.1038/202928a0
  14. Lee, K.D., J. Kim, and H. Kim. 1999. Purification and characterization of carboxymethyl-cellulase produced by Bacillus sp. KD1014. Agric. Chem. Biotechnol. 42:107-112.
  15. Lim, D.K., Y.H. Moon, I.S. Shin, and K.D. Woo. 1991. Development of organic fertilizer based on the cow dung: 2. Studies on rapid fermentation. Korean J. Soil Sci. Fert. 24:199-205.
  16. MAFF (Ministry of Food, Agriculture, Forestry and Fisheries). 2010. Discharges amounts of livestock manure and resource recycling. p.1-30.
  17. Martin, J.P. 1950. Use of acid, rose bengal and streptomycin in the plate method for estimating soil fungi. Soil Sci. 69:215-233. https://doi.org/10.1097/00010694-195003000-00006
  18. Miller, G.L. 1959. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal. Chem. 31:426-428. https://doi.org/10.1021/ac60147a030
  19. Misra, R.V., R.N. Roy, and H. Hiraoka. 2003. On-farm composting methods. p. 1-35. Food and agriculture organization of the United nations (FAO), Rome, Italy.
  20. NIAST, 2000. Method of soil and plant analysis. Published by National Institute of Agricultural Science & Technology, Suwon, Korea.
  21. Oh, Y.S., J.E. Park, H.J. Oh, J.H. Kim, M.C. Oh, C.K. Oh, Y.J. Oh, and S.B. Lim. 2010. Isolation and characteristics of microorganisms producing extracellular enzymes from Jeju traditional fermented soybean paste (Doenjang). J. Korean Soc. Food Sci. Nutr. 39:47-53. https://doi.org/10.3746/jkfn.2010.39.1.047
  22. Park, J.H., J.K. Yeo, Y.B. Koo, W.W. Lee, H.C. Kim, and C.H. Park. 2008. Effects of slurry composting and biofiltration liquid fertilizer on growth characteristic of poplar clones in a reclaimed land mounding soil. Korean J. Soil Sci. Fert. 41:318-323.
  23. Tchobanoglous, G., H. Theisen, and S.A. Vigil. 1993. Integrated solid waste management, p. 689-691 In: Engineering, principles and management issues. McGraw-Hill International Editions.
  24. Tonkova, A., R. Manolov, and D. Elena. 1993. Thermostable $\alpha$-amylase from derepressed Bacillus licheniformis produce in high yields from glucose. Process Biochem. 28:539-542. https://doi.org/10.1016/0032-9592(93)85015-8
  25. Tyrrell, H.F., A.C.G. Brown, P.J. Reynolds, G.L. Haaland, D.E. Bauman, C.J. Peel, and W.D. Steinhour. 1988. Effect of bovine somatotrophin on metabolism of lactating dairy cows: energy and nitrogen utilisation as determined by respiration calorimetry. J. Nutr. 118:1024-1030. https://doi.org/10.1093/jn/118.8.1024
  26. Van Weerden, E.J. 1987. Effects of clenbuterol on nitrogen deposition and carcass composition in castrated male pigs. p. 152-162. In: Hanrahan, J.P., Beta-agonists and their effects on animal growth and carcass quality. commission of the European communities. Elsevier applied science, UK.