The Journal of Internal Korean Medicine (대한한방내과학회지)

The Society of Internal Korean Medicine (대한한방내과학회)
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Effects of Ethanol Extract of Benincasa Seeds on the Experimental Cellular Model of Nonalcoholic Fatty Liver Disease

동과자 에탄올 추출물이 비알코올성 지방간 세포 모델에 미치는 효과

  • Choi, Jun-Young (Dept. of Internal Medicine, School of Korean Medicine, Pusan National University) ;
  • Kim, So-Yeon (Dept. of Internal Medicine, School of Korean Medicine, Pusan National University) ;
  • Kwun, Min-Jung (Division of Applied Medicine, School of Korean Medicine, Pusan National University) ;
  • Kim, Kyun-Ha (Division of Applied Medicine, School of Korean Medicine, Pusan National University) ;
  • Joo, Myung-Soo (Division of Applied Medicine, School of Korean Medicine, Pusan National University) ;
  • Han, Chang-Woo (Dept. of Internal Medicine, School of Korean Medicine, Pusan National University)
  • 최준용 (부산대학교 한의학전문대학원 내과학교실) ;
  • 김소연 (부산대학교 한의학전문대학원 내과학교실) ;
  • 권민정 (부산대학교 한의학전문대학원 응용의학부) ;
  • 김균하 (부산대학교 한의학전문대학원 응용의학부) ;
  • 주명수 (부산대학교 한의학전문대학원 응용의학부) ;
  • 한창우 (부산대학교 한의학전문대학원 내과학교실)
  • Published : 2012.12.30
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Abstract

Objectives : In this study, we investigated the effect and the underlying mechanism of ethanol extract of Benincasa seeds on a cellular model of non-alcoholic fatty liver disease (NAFLD) established by treating HepG2 cells with palmitate. Methods : We evaluated ethanol extract of Benincasa seeds (EEBS) for its hepatic lipid-lowering potential in fatty acid overloaded HepG2 cells. After incubation in palmitate containing media with or without EEBS, intracellular neutral lipid accumulations were quantified by Nile red staining. We also investigated the effect of EEBS on lipogenesis and ${\beta}$-oxidation. $LXR{\alpha}$-dependent SREBP-1c activation, expression of lipogenic genes, and expression of ${\beta}$-oxidation related genes were determined with or without pretreatment of EEBS. Results : EEBS significantly attenuated palmitate-induced intracellular neutral lipid accumulation in HepG2 cells. EEBS suppressed fatty acid synthesis by inhibiting $LXR{\alpha}$-dependent SREBP-1c activation. EEBS also repressed SREBP-1c mediated induction of lipogenic genes, including ACC, FAS, and SCD-1. However, EEBS had no effect on ${\beta}$-oxidation related CPT-1 and $PPAR{\alpha}$ gene expression. Conclusions : Our results suggest that EEBS has an efficacy to decrease hepatic lipid accumulation, and this effect was mediated by inhibiting the $LXR{\alpha}$-SREBP-1c pathway that leads to expression of lipogenic genes and hepatic steatosis. Therefore, the Benincasa seeds may have a potential clinical application for treatment of this chronic liver disease.

Keywords

References

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