Food Science of Animal Resources (한국축산식품학회지)

Korean Society for Food Science of Animal Resources (한국축산식품학회)
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A Method for Measuring Lipid Peroxidation of Freeze-dried Egg Yolk by Using Chemiluminescence Analyzer

화학발광분석기를 이용한 동결건조 난황분말의 산패도 측정법

  • Pyun, Chang-Won (Department of Food Science and Biotechnology of Animal Resources, Konkuk University) ;
  • Hong, Go-Eun (Department of Food Science and Biotechnology of Animal Resources, Konkuk University) ;
  • Jang, Soon-Hong (Department of Food Science and Biotechnology of Animal Resources, Konkuk University) ;
  • Kim, Jong-Min (Department of Food Science and Biotechnology of Animal Resources, Konkuk University) ;
  • Kim, Soo-Ki (Department of Animal Science and Environment, Konkuk University) ;
  • Lee, Chi-Ho (Department of Food Science and Biotechnology of Animal Resources, Konkuk University)
  • 변장원 (건국대학교 축산식품생물공학과) ;
  • 홍고은 (건국대학교 축산식품생물공학과) ;
  • 장순홍 (건국대학교 축산식품생물공학과) ;
  • 김종민 (건국대학교 축산식품생물공학과) ;
  • 김수기 (건국대학교 동물생산.환경학과) ;
  • 이치호 (건국대학교 축산식품생물공학과)
  • Received : 2011.07.21
  • Accepted : 2012.01.31
  • Published : 2012.02.29
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Abstract

The aim of this study was to investigate the relationship between a simple method for measuring lipid peroxidation by using a chemiluminescence analyzer and traditional methods, such as 2-thiobarbituric acid reactive substances or peroxide value of solid food samples. Freeze-dried egg yolk powder was kept on $25^{\circ}C$, under dark condition. The peroxidation value was measured during certain storage period by using 3 methods, and the chemiluminescence value was compared with 2-thiobarbituric acid reactive substances or peroxide value. For comparison, 3 kinds of freeze-dried egg yolk were prepared from whole eggs purchased from a local market. The chemiluminescence value was significantly correlated with both the peroxide value and the 2-thiobarbituric acid related substances during storage, and showed a high correlation to the real sample test. It showed a little higher correlation with peroxide value. These results suggest that using a chemiluminescence analyzer may provide the ability to measure the lipid peroxidation of high lipid content solid-food samples, instead performing both the 2-thiobarbituric acid reactive substances test and measuring the peroxide value.

본 연구는 TBARS 및 PV 등의 식품중의 지질산패도 측정법을 대체할 수 있는 간단한 측정법을 개발하고자 시행되었다. 이를 위해 선행 연구에서 식용유지의 산패도 측정법 대체가능성을 나타낸 화학발광도 측정법을 고체시료 중에서 축산식품인 동결건조 난황분말에 적용하여 기존의 산패도 측정법과의 상관관계를 조사하여 대체 가능성을 확인하였다. 암소의 $25^{\circ}C$에서 저장기간(0, 12, 24, 48, 72, 144시간) 별로 시료를 채취해 TBARS와 PV 및 화학발광도를 측정하여 비교하였다. 저장시간에 따른 화학발광도 측정 결과는 특히 과산화물가 측정치와 유의적으로 높은 상관관계를 나타내었으며($r^2=0.987$), 상대적으로 높지는 않지만($r^2=0.874$) TBA가와도 유의적으로 상관관계가 있다고 조사되었다. 또한 실제 표준시료를 이용한 분석 결과에서도 유의적으로 높은 상관관계($r^2=0.9214$, 0.9599)를 나타내었다. 따라서 화학발광도를 이용한 지질 산패도 측정법은 여러 식품 등의 지질 산패도 측정에 있어서 POV를 대체할 수 있을 것으로 기대가 되며, 경우에 따라서는 TBARS에 대해서도 대체가 가능할 것으로 기대된다.

Keywords

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