Mass Spectrometry Letters

Korean Society for Mass Spectrometry (한국질량분석학회)
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Observation of Peptide-Ion Generation by Laser-Induced Surface Heating from Tungsten Silicide Surfaces

  • Kim, Shin-Hye (Center for Nano-Bio Technology, Korea Research Institute of Standards and Science) ;
  • Park, Sun-Hwa (Center for Nanocharacterization, Korea Research Institute of Standards and Science) ;
  • Song, Jae-Yong (Center for Nanocharacterization, Korea Research Institute of Standards and Science) ;
  • Han, Sang-Yun (Center for Nano-Bio Technology, Korea Research Institute of Standards and Science)
  • Received : 2012.02.27
  • Accepted : 2012.03.06
  • Published : 2012.03.15
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Abstract

We report observation of laser desorption/ionization (LDI) of peptides from flat surfaces of tungsten silicide ($WSi_2$). In contrast to MALDI (matrix-assisted laser desorption/ionization) and SALDI (surface-assisted laser desorption/ionization) mass spectrometry, this study did not utilize any matrices and surface nanostructures. In this work, LDI on $WSi_2$ surfaces is demonstrated to cover a mass range up to 1,600 Da (somatostatin; monoisotopic mass = 1637.9 Da). In addition, it exhibited a high sensitivity, which could detect peptides, which could detect peptides of low femtomole levels (20 fmol for angiotensin II). The observed LDI process was discussed to be largely thermal, more specifically, due to laser-induced surface heating that is most likely promoted by the low thermal diffusivity (${\kappa}$) of $WSi_2$ substrate.

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