Fisheries and Aquatic Sciences

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Metabolic Activity of Desalted Ground Seawater of Jeju in Rat Muscle and Human Liver Cells

  • Kim, Bo-Youn (Department of Medicine, School of Medicine, Jeju National University) ;
  • Lee, Young-Ki (Department of Medicine, School of Medicine, Jeju National University) ;
  • Park, Deok-Bae (Department of Medicine, School of Medicine, Jeju National University)
  • Received : 2011.12.09
  • Accepted : 2012.02.08
  • Published : 2012.03.30
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Abstract

Ground seawater in the east area of the volcanic Jeju Island contains abundant minerals. We investigated the metabolic activity of electrodialyzed, desalted ground seawater (EDSW) from Jeju in both cultured cells and animals. The addition of EDSW to the culture medium (up to 20%, v/v) reduced the leakage of lactate dehydrogenase and increased MTT activity in CHO-IR cells. EDSW (10%) promoted insulin-induced glucose consumption in L6 muscle cells as well as the activities of the liver ethanol-metabolizing enzymes, alcohol dehydrogenase and aldehyde dehydrogenase. Moreover, EDSW suppressed palmitate-induced intracellular fat accumulation in human hepatoma $HepG_2$ cells. Activities of AMP-stimulated protein kinase and acetyl CoA carboxylase, enzymes that modulate fat metabolism, were altered by EDSW in $HepG_2$ cells toward the suppression of intracellular lipid accumulation. EDSW also suppressed hepatic fat accumulation induced by a high-fat diet in mice. Taken together, EDSW showed beneficial metabolic effects, including the enhancement of ethanol metabolism and insulin-induced glucose consumption, and the suppression of intrahepatic fat accumulation.

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References

  1. Antonescu CN, Foti M, Sauvonnet N and Klip A. 2009. Ready, set, internalize: mechanisms and regulation of GLUT4 endocytosis. Biosci Rep 29, 1-11. https://doi.org/10.1042/BSR20080105
  2. Barbagallo M and Dominguez LJ. 2007. Magnesium metabolism in type 2 diabetes mellitus, metabolic syndrome and insulin resistance. Arch Biochem Biophys 458, 40-47. https://doi.org/10.1016/j.abb.2006.05.007
  3. Barbagallo M, Dominguez LJ and Resnick LM. 2007. Magnesium metabolism in hypertension and type 2 diabetes mellitus. Am J Ther 14, 375-385. https://doi.org/10.1097/01.mjt.0000209676.91582.46
  4. Bostian KA and Betts GF. 1978. Rapid purification and properties of potassium-activated aldehyde dehydrogenase from Saccharomyces cerevisiae. Biochem J 173, 773-786. https://doi.org/10.1042/bj1730773
  5. Chen MD and Lin PY. 2000. Zinc-induced hyperleptinemia relates to the amelioration of sucrose-induced obesity with zinc repletion. Obes Res 8, 525-529. https://doi.org/10.1038/oby.2000.65
  6. De Bolle X, Vinals C, Fastrez J and Feytmans E. 1997. Bivalent cations stabilize yeast alcohol dehydrogenase I. Biochem J 323(Pt 2), 409-413. https://doi.org/10.1042/bj3230409
  7. Dickinson FM. 1996. The purification and some properties of the Mg(2+)-activated cytosolic aldehyde dehydrogenase of Saccharomyces cerevisiae. Biochem J 315(Pt 2), 393-399. https://doi.org/10.1042/bj3150393
  8. Havelaar AH and Melse JM. 2003. Quantifying Public Health Risks in the WHO Guidelines for Drinking-Water Quality: A Burden of Disease Approach. RIVM Report 734301022. National Institute of Public Health and the Environment, Bilthoven, NL.
  9. Hirano T, Abe K, Gotoh M and Oka K. 1995. Citrus flavone tangeretin inhibits leukaemic HL-60 cell growth partially through induction of apoptosis with less cytotoxicity on normal lymphocytes. Br J Cancer 72, 1380-1388. https://doi.org/10.1038/bjc.1995.518
  10. Katsuda S, Yasukawa T, Nakagawa K, Miyake M, Yamasaki M, Katahira K, Mohri M, Shimizu T and Hazama A. 2008. Deep-sea water improves cardiovascular hemodynamics in Kurosawa and Kusanagi-Hypercholesterolemic (KHC) rabbits. Biol Pharm Bull 31, 38-44. https://doi.org/10.1248/bpb.31.38
  11. Kobayashi J. 1957. On geographical relationship between the chemical nature of river water and death-rate from apoplexy. Ber Ohara Inst Landwirtsch Biol Okayama Univ 11, 12-21.
  12. Koh YB, Lee YD and Kim YH. 2008. Final Report of Technological Development Project of Regional Industry (Project No. 10025071): Development of Industrial Resources and Products from Jeju Ground Seawater. Jeju Technopark, Jeju, KR, pp. 214-250.
  13. Miyamura M, Yoshioka S, Hamada A, Takuma D, Yokota J, Kusunose M, Kyotani S, Kawakita H, Odani K, Tsutsui Y and Nishioka Y. 2004. Difference between deep seawater and surface seawater in the preventive effect of atherosclerosis. Biol Pharm Bull 27, 1784-1787.
  14. Mosmann T. 1983. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 65, 55-63. https://doi.org/10.1016/0022-1759(83)90303-4
  15. Niefind K, Müller J, Riebel B, Hummel W and Schomburg D. 2003. The crystal structure of R-specific alcohol dehydrogenase from Lactobacillus brevis suggests the structural basis of its metal dependency. J Mol Biol 327, 317-328. https://doi.org/10.1016/S0022-2836(03)00081-0
  16. Rylander R and Arnaud MJ. 2004. Mineral water intake reduces blood pressure among subjects with low urinary magnesium and calcium levels. BMC Public Health 4, 56. https://doi.org/10.1186/1471-2458-4-56
  17. Santos JM, Ribeiro SB, Gaya AR, Appell HJ and Duarte JA. 2008. Skeletal muscle pathways of contraction-enhanced glucose uptake. Int J Sports Med 29, 785-794. https://doi.org/10.1055/s-2008-1038404
  18. Schoppen S, Perez-Granados AM, Carbajal A, Sarria B, Sanchez-Muniz FJ, Gomez-Gerique JA and Pilar Vaquero M. 2005. Sodium bicarbonated mineral water decreases postprandial lipaemia in postmenopausal women compared to a low mineral water. Br J Nutr 94, 582-587. https://doi.org/10.1079/BJN20051515
  19. Tjio JH and Puck TT. 1958. Genetics of somatic mammalian cells. II. Chromosomal constitution of cells in tissue culture. J Exp Med 108, 259-268. https://doi.org/10.1084/jem.108.2.259
  20. Ueshima S, Fukao H, Okada K and Matsuo O. 2003. Suppression of the release of type-1 plasminogen activator inhibitor from human vascular endothelial cells by Hawaii deep sea water. Pathophysiology 9, 103-109. https://doi.org/10.1016/S0928-4680(02)00076-7
  21. Yoshioka S, Hamada A, Cui T, Yokota J, Yamamoto S, Kusunose M, Miyamura M, Kyotani S, Kaneda R, Tsutsui Y, Odani K, Odani I and Nishioka Y. 2003. Pharmacological activity of deep-sea water: examination of hyperlipemia prevention and medical treatment effect. Biol Pharm Bull 26, 1552-1559. https://doi.org/10.1248/bpb.26.1552