Korean Journal of Poultry Science (한국가금학회지)

The Korean Society of Poultry Science (한국가금학회)
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Effects of N-Methylacetamide on the Viability, Fertility and Hatchability of Cryopreserved Ogye (Korean Native Black Fowl) Semen

N-Methylacetamide 동결보호제가 오계 동결정액의 생존성, 수정 및 부화율에 미치는 영향

  • Choi, Jin Seok (Animal Genetic Resources Station, National Institute of Animal Science, RDA) ;
  • Kim, Sung Woo (Animal Genetic Resources Station, National Institute of Animal Science, RDA) ;
  • Shin, Dan-Bi (Animal Genetic Resources Station, National Institute of Animal Science, RDA) ;
  • Ko, Yeoung-Gyu (Animal Genetic Resources Station, National Institute of Animal Science, RDA) ;
  • Do, Yoon-Jung (Animal Genetic Resources Station, National Institute of Animal Science, RDA) ;
  • Kim, Dong-Hun (Animal Genetic Resources Station, National Institute of Animal Science, RDA) ;
  • Kong, Il-Keun (Department of Animal Science, Institute of Agriculture and Life Science, Graduate School of Gyengsang National University) ;
  • Park, Soo-Bong (Animal Genetic Resources Station, National Institute of Animal Science, RDA)
  • 최진석 (농촌진흥청 국립축산과학원 가축유전자원시험장) ;
  • 김성우 (농촌진흥청 국립축산과학원 가축유전자원시험장) ;
  • 신단비 (농촌진흥청 국립축산과학원 가축유전자원시험장) ;
  • 고응규 (농촌진흥청 국립축산과학원 가축유전자원시험장) ;
  • 도윤정 (농촌진흥청 국립축산과학원 가축유전자원시험장) ;
  • 김동훈 (농촌진흥청 국립축산과학원 가축유전자원시험장) ;
  • 공일근 (경상대학교 농업생명과학대학 축산학과) ;
  • 박수봉 (농촌진흥청 국립축산과학원 가축유전자원시험장)
  • Received : 2012.11.01
  • Accepted : 2012.12.04
  • Published : 2012.12.31
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Abstract

The use of methylacetamide (MA) as a cryoprotective agent for freezing Korean Native Black rooster Ogye semen was examined with artificial insemination. The diluted Ogye semen with HS-1 was subjected for 2 step dilution method of cryopreservation in which the final concentration of MA was adjusted to 7.5%. The sperm viability after thawing was reduced from $95.17{\pm}0.93%$ to $55.93{\pm}1.38%$ which was confirmed by live-death analysis based on Fluorescence-Activated Cell Sorting (FACS). The rates of fertilized eggs with fresh or frozen-thawed semen were reduced from $94.98{\pm}3.93%$ to $66.36{\pm}8.43%$ at day 7 with significant difference. However, the hatching rates of experiments at day 21 did not shown difference between $92.64{\pm}2.33%$ and $90.45{\pm}8.05%$ (P<0.05). With these results, the utilization of MA for freezing of Ogye spermatozoa could affect on viability of frozen-thawed semen but not on the fertility of lain eggs and hatchability of fertilized eggs and also provide possible tools of freezing for poultry genetic resource conservation.

본 연구는 동결보호제로 이용되는 MA가 오계 정액의 생존율과 생산된 종란의 수정 및 부화율에 미치는 영향을 검토하여 재래가금의 동결보존 방법의 기초를 마련하고자 실시하였다. 10수의 오계 수탉에서 채취한 혼합정액을 희석정액을 제조하고 동시에 동일한 희석액으로 준비된 신선정액을 준비하여 각각 20수의 White Leghorn 암탉에 인공수정하였다. 7일 동안 생산된 종란을 각각 114개 및 115개를 회수하여 인공부화기에 입란하였고, 발생 7일째에 검란하여 수정율을 관찰하였으며, 발생 21일째에 부화율과 발육 중지란을 조사하였다. 동결정액을 이용하여 생산된 종란의 수정율은 66.36%로 관찰되었으며, MA가 함유된 희석액으로 준비한 동일한 농도의 신선 정액을 이용하여 생산된 종란의 수정율은 94.98%로 관찰되었다. 또한, MA 동결보호제가 함유된 희석정액과 동결정액을 이용하여 생산된 종란의 부화율은 모두 90% 이상으로 관찰되어 유의성이 없었으며, Glycerol 동결보호제의 문제점으로 보고된 수정 능력 및 부화 억제 효과는 없는 것으로 사료된다. 그러므로 동결정액을 이용한 종란에서 무정란 수는 신선한 희석 정액을 사용하였을 때보다 약 6.3배로 유의적 높았으나, 동결보호제에 의한 효과로 추정되지는 않았다. 닭 동결정액의 기술의 실용화를 위해서는 조류 정액의 보존 및 동결 연구에는 여전히 해결되어야 할 문제점이 많으며, 특히 품종 간 및 개체 간의 변이가 크기 때문에 앞으로 더 많은 연구가 필요하다고 사료된다.

Keywords

References

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  1. Effects of Kinds of Cryoprotectants on the Characteristics of Frozen Fowl Semen vol.40, pp.3, 2013, https://doi.org/10.5536/KJPS.2013.40.3.171