Journal of Oriental Neuropsychiatry (동의신경정신과학회지)

The Korean Society of Oriental Neuropsychiatry (대한한방신경정신과학회)
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Preventive Effects of Santalum album L. Extracts on Oxidation, Platelet Aggregation and Thrombosis

백단향추출물의 항산화, 항혈소판 응집 및 혈전 용해능에 관한 연구

  • Sung, Yung-Wei (Dept. of Neuropsychiatry, College of Oriental Medicine, Dong-Guk University) ;
  • Lee, Ji-Hyun (Dept. of Neuropsychiatry, College of Oriental Medicine, Dong-Guk University) ;
  • Song, Kyoo-Ju (Dept. of Neuropsychiatry, College of Oriental Medicine, Dong-Guk University) ;
  • Koo, Byung-Soo (Dept. of Neuropsychiatry, College of Oriental Medicine, Dong-Guk University) ;
  • Kim, Geun-Woo (Dept. of Neuropsychiatry, College of Oriental Medicine, Dong-Guk University)
  • 송영위 (동국대학교 한의과대학 한방신경정신과학교실) ;
  • 이지현 (동국대학교 한의과대학 한방신경정신과학교실) ;
  • 송규주 (동국대학교 한의과대학 한방신경정신과학교실) ;
  • 구병수 (동국대학교 한의과대학 한방신경정신과학교실) ;
  • 김근우 (동국대학교 한의과대학 한방신경정신과학교실)
  • Received : 2012.03.05
  • Accepted : 2012.03.14
  • Published : 2012.03.30
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Abstract

Objectives : To evaluate the in vitro scavenging activity, inhibitory effect of LDL oxidation of pro-oxidant reactive species, anti-platelet aggregative effects and anti-thrombosis effects in response to treatment with SA using various screening methods including biological and non-biological oxidants. Methods : The antioxidant activity concerning extract from SA was studied with in vitro methods by measuring the antioxidant activity by TEAC, measuring the scavenging effects on reactive oxygen species (ROS) [superoxide anion, hydroxyl radical] and on reactive nitrogen species (RNS) [nitric oxide and peroxynitrite] as well as measuring the inhibitory effect on $Cu^{2+}$-induced human LDL oxidation and the inhibitory effect on thrombin-induced platelet aggregation and thrombosis. Results : SA extracts were found to have a potent scavenging activity regarding oxidative stress as well as an inhibitory effect towards LDL oxidation, platelet aggregation, and thrombosis. Conclusions : The SA extracts have anti-oxidative and anti-atherosclerotic effects in vitro system, which can be used for developing pharmaceutical drugs against oxidative stress and atherosclerosis.

Keywords

References

  1. Chance, B, Sies, H, Boveris, A. Hydroperoxide metabolism in mammalian organs. Physiol. Rev. 1979; 59: 527-605.
  2. Wickens, A.P. Ageing and the free radical theory. Respir. Physio. 2001; 128:379-391. https://doi.org/10.1016/S0034-5687(01)00313-9
  3. Amarowicz, R., Naczk, M. and Shahidi, F. Antioxidant activity of various fractions of nontannin phenolics of canola hulls. J. Agric. Food Chem. 2000; 48: 2755-2759. https://doi.org/10.1021/jf9911601
  4. Frank, M.S. and Martijn, K. Randomized clinical trials on the effects of dietary fat and carbohydrate on plasma lipoprotein and cardiovascular disease. Am. J. Med. 2002;, 113: 13-24. https://doi.org/10.1016/S0002-9343(01)00987-1
  5. Sang Bae Kim. Development of Thrombo-Q, Food for antithrombotic and hyperlipidemia. The Korean Society of Food Science and Nutrition 2000; 5(1): 57-60.
  6. Wolberg, A.S. and Cmpbell, R.A, Thrombin generation, fibrin clot formation and hemostasis. Transfus. Apher. Sci ,2008; 38(1): 15-23. https://doi.org/10.1016/j.transci.2007.12.005
  7. Verstraeta, M., Lijnen, H.R. and Collen, D. Thrombolytic agents in development. Drugs 1995; 50:29-42. https://doi.org/10.2165/00003495-199550010-00003
  8. Pyong-chol Pae. Kumsok Hwangjenaegyong Somun. Seongbosa; 1999. 533 ; 186; 80-82.
  9. Nam In Jo, Seung heon Choi, Kyoo Seok Ahn, Effects of Dansameum and each its components on Intravascular Coagulation Induced by Endotoxin Rats. The journal of K.O.M.S. 1975;12(2):66-77.
  10. Dongyeol Kwak, Jae-won Kim. Uirimkaechakpyongyok. Changjo Munwhasa; 1998. p. 259.
  11. Chonguk haniqwa taehak ponchohak kyosu. Ponchohak. Yongnimsa; 1988. 1-813, 362.
  12. Tae-Hoon Kim, Antioxidative and Biological Activities of Santalum album Extracts by Extracting Methods: The Korean Society of Food Preservation 2008; 15(3): 456-460.
  13. Kujala, T.S., Loponen, J.M., Klika, K.D. and Pihlaja, K. Phenolic and betacyanins in red beetroot (Beta vulgaris) root: distribution and effects of cold storage on the content of total phenolics and three individual compounds. J. Agri. Food Chem., 2000. p. 48: 5338-5342. https://doi.org/10.1021/jf000523q
  14. Roberta, R.E., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M. and Rice-Evans, C. Antioxidant activity applying an improved ABTS radical cation decolorization assay., Free Radical Bio. Med., 1999. p. 26: 1231-1237. https://doi.org/10.1016/S0891-5849(98)00315-3
  15. Miller, N.J., Rice-Evans, C., Davies, M.J., Gopinathan, V. and Milner, A.A. A novel method for measuring antioxidant capacity and its application to monitoring the antioxidant status in premature neonates. Clin. Sci., 1993. p. 84: 407-412,
  16. Blois, M.S. Antioxidant determination by the use of stable free radical. Nature; 1958. p. 26: 1199-1200.
  17. Gotoh, N. and Niki, E., Rates of interactions of superoxide with vitamin E, vitamin C and related compounds as measured by chemiluminescence. Biochem. Biophys. Acta; 1992. p. 1115: 201-207. https://doi.org/10.1016/0304-4165(92)90054-X
  18. Halliwell, B. and Gutteridge, J.M. Role of free radicals and catalytic metalions in human disease: an overview. Method Enzymol.; 1990. p. 186: 1-85. https://doi.org/10.1016/0076-6879(90)86093-B
  19. Nagata, N., Momose, K. and Ishida, Y. nhibitory effects of catecholamines and anti-oxidants on the fluorescence reactionof 4,5-diaminofluorescein, DAF-2, a novel indicator of nitric oxide. J. Biochem. (Tokyo); p. 125: 658-661. https://doi.org/10.1093/oxfordjournals.jbchem.a022333
  20. Crow, J.P. Dichlorodihydrofluorescein and dihydrorhodamine 123 are sensitive indicators of peroxynitrite in vitro: implications for intracellular measurement of reactive nitrogen and oxygen species. Nitric Oxide, 1997. p. 1: 145-157. https://doi.org/10.1006/niox.1996.0113
  21. Hu, C., Zhang, Y. and Kitts, D.D. Evaluation of antioxidant and prooxidant activities of bamboo Phyllostachys nigra var. Henonis leat extract in virto. J. Agric. Food Chem; 2000. p. 48: 3170-3146. https://doi.org/10.1021/jf0001637
  22. Wiseman, H. Important in protection against oxidative damage and disease. Nutr. Biochem.; 1996. p.7: 2-6. https://doi.org/10.1016/0955-2863(95)00152-2
  23. Wickens, A.P. Ageing and the free radical therory. Respir. Physiol. 128: 379-391, 2001. https://doi.org/10.1016/S0034-5687(01)00313-9
  24. Dongyeol Kwak, Jae-won Kim. Uirimkaechakpyongyok. Changjo Munwhasa; 1998. p. 260-262.
  25. Chunwong Huh. Knowing the cause, we can see the prevention of cerebrovascular disease. M.S; 2003. p. 81.
  26. Gaebin Zhang. Gyongakjeonseo. Sanghaesanghae Kwahak Kisul Chulpansa 1985. 71-578.
  27. Satake Zhin. Byunzheungnok. Seoul: Uisongdang. 241-6, 1989.
  28. Jeonkuk Hanigwa Daehak Singyong chongsinkwa Kyogwaso Pyonchan Wiwonhoe .Hani Singyong chongsinkwahak. Kyonggi-do: Chimmundang; 2007. p. 324-333; 237-328.
  29. Hangmun Zhang. Chungpung Chimaezheung Byunggi Tamto. Habseozhungyihakbo. 1997; 20(1) :1-3.
  30. Juni Kim, Bongin Kim, Jinkyoung Kim. Study about preference of aroma 2003; 8:158-161.
  31. Bong-Jeun An, Man-Jong Bae, Hee-Jin Choi, Yun-Bin Zhang, Tae-Soo Sung and Cheoing Choi. Isolation of Polyphenol Compounds from the Leaves of Korean Persimmon (Diospyrus kaki L. Folium): The Korean Society for Applied Biological Chemistry, 45(4): 212-217, 2002.
  32. Ahmad, N., Gupta, S. and Mukhtar, H. Green tea polyphenol epigallocatechin-3- gallate differentially modulates nuclear factor ĸB in cancer cells versus normal cells. Arch. Biochem. Biophys., 376: 338-346, 2000. https://doi.org/10.1006/abbi.2000.1742
  33. Hye-Suk Kim, Mi-jeong Hong, In-Yeong Kang, Ji-Youn Jung, Hye-Kyung Kim, Yong-Seub Shin, et al. Radical Scavening Activities and Antioxidant Constituents of Oriental Melon Extract. Journal of Bio-Environment Control 2009; 18(4): 442-447.
  34. Awika, J.M., Rooney, L.W., Wu, X.L., Prior, R.L. and Cisneros-Zevallos, L. Screening methods to measure antioxidant activity of sorghum (Sorghum bicolor) and sorghum products. J. Agric. Food Chem., 51: 6657-6662, 2003. https://doi.org/10.1021/jf034790i
  35. Sanchez, C.S., Gonzalez, A.M.T., Garcia-Parrilla, M.C., Granados, J.J.Q., Serrana, H.L.G. and Martinez, M.C.L. Different radical scavenging tests in virgin olive oil and their relation to the total phenol content. Anal. Chim. Acta., 593: 103-107, 2007. https://doi.org/10.1016/j.aca.2007.04.037
  36. Yokozawa, T., Chen, C.P., Dong, E., Tanaka, T., Nonaka, G.I. and Nishioka, I. Study on the Inhibitory effect of tannins and flavonoids against the 1,1-diphenyl-2-picrylhydrazyl radical. Biochem. Pharmacol., 56(2):213-222, 1998.
  37. Magalhaes, L.M., Segundo, M.A. and Reis, S. Automatic method for determination of total antioxidant capacity using 2,2-diphenyl-1- picrylhydrazyl assay. Anal. Chinm. Acta., 558, 310-318, 2006. https://doi.org/10.1016/j.aca.2005.11.013
  38. Blois, M.S. Antioxidant determination by the use of a stable free radical. Nature, 181: 1199-1200, 1958. https://doi.org/10.1038/1811199a0
  39. Trush, M. A. , Mimnaugh, E.G. and Gram, T.E. Activation of pharmacologic agents to radical intermediates. Implications for the role of free radicals in drug action and toxicity. Biochem. Pharmacol., 31: 3335-3346, 1982. https://doi.org/10.1016/0006-2952(82)90609-8
  40. Aust, S.D., Morehouse, L.A. and Tomas, C.E. Role of metals in oxygen radical reactions. J. Free Radicals Biol. Med., 1:3-25, 1985. https://doi.org/10.1016/0748-5514(85)90025-X
  41. Aruoma, O.I. Nutrition and health aspects of free radicals and antioxidants. Food Chem. Toxicol., 32: 671-683, 1994. https://doi.org/10.1016/0278-6915(94)90011-6
  42. Davies, K.J. and Goldberg, A.L. Proteins damaged by oxygen radicals are rapidly degraded in extracts of red blood cells. J. Biol. Chem., 262: 8227-8234, 1987.
  43. Sung-Je Jung, Jin-Hee Lee, Hyo-Nam Song, Nak-sul Seong, Seung-Eun Lee and Nam-In Baek, Screening for Antioxidant Activity of Plant Medicinal Extracts: The Korean Society for Applied Biological Chemistry. 47(1): 135-140, 2004.
  44. Patel, R.P., McAndrew, J., Sellak, H., White, C.R., Jo, H., Freeman, B.A. and Darley-Usmar, V.M. Biological aspects of reactive nitrogen species. BBA-Bioenergetics, 1411: 385-400, 1999. https://doi.org/10.1016/S0005-2728(99)00028-6
  45. Liang, Y.C., Huang, Y.T., Tsai, S.H., Lin-shiau, S.Y., Chen, C.F. and Lin, J.K. Suppression of inducible cyclooxygenase and inducible nitric oxide synthase by apigenin and relted flavonoids in mouse macrophase. Carcinogenesis, 20: 1945-1952, 1999. https://doi.org/10.1093/carcin/20.10.1945
  46. Min Jung Jang, Bong Jeun An, Chang Eeon Lee, Jin Tae Lee, Byung Guen Lee, Do Hyung Lee. Study on the Anti-oxidant Effect of Pinus rigida Mill. inner Bark Extracts. Jour. Korean For. Soc. 2008;97(1):88-94.
  47. Hye-Young Min, Eun-jung Park, Sang-Kook Lee, Yong-Jin Cho. Effects of Grape Extracts on Free Radical Scavenging Activity and Inhibition of Pro-Inflammatory Mediator Production in Mouse Macrophage Cells. Korean J. Food SCI. Technol. 2003;35(1):132-137.
  48. Sun-Ok Jee. Antioxidant Acivities and Whitening Effect of the Mulberry(Morus alba L.) Root Bark Extracts. Korean J. Plant Res 2009; 22(2): 145-151.
  49. Moran, N. and Fitzgerald, G. A. 1994. Mechanism of action of antiplatelet drugs. In: Colman, R.W., Hirsh, J., Marder, V.J. and Salzman, E.W. (ed.). Hemostasis & Thrombosis, 3rd edition. Philadelphia, JB: Lippincott, pp 1623-1637.
  50. Mustard, J.F. and Packham, M.A. Factors influencing platelet function: adhesion, release and aggregation. Pharmacol. Rev., 22: 97-137, 1970.
  51. Ki Hyun Cho, Sung Min Choi, Byong Chae Kim, Myong Kyoo Kim, Ui Joo Son, Jong Hee Nam.. An Open, Comparative Study of the Inhibitory Effect on Platelet Aggregatino and Safety of Clopidogrel and Aspirin in Patients with Ischemic Stroke. J. of K.S.C.P.T. 2001; 9(2): 197-205.
  52. Quinn, M.J. and Fitzgerald, D.J. Ticlopidine and clopidogrel. Circulation. 100: 1667-1672, 1999. https://doi.org/10.1161/01.CIR.100.15.1667

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