Asian Pacific Journal of Cancer Prevention

Asian Pacific Journal of Cancer Prevention (아시아태평양암예방학회)
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Iris Nertschinskia Ethanol Extract Differentially Induces Cytotoxicity in Human Breast Cancer Cells Depending on AKT1/2 Activity

  • Shin, Jae-Sik (Innovative Cancer Research, Asan Institute for Life Science, Asan Medical Center) ;
  • Maeng, Hyung-Gun (Innovative Cancer Research, Asan Institute for Life Science, Asan Medical Center) ;
  • Hong, Seung-Woo (Innovative Cancer Research, Asan Institute for Life Science, Asan Medical Center) ;
  • Moon, Jai-Hee (Innovative Cancer Research, Asan Institute for Life Science, Asan Medical Center) ;
  • Kim, Jin-Sun (Innovative Cancer Research, Asan Institute for Life Science, Asan Medical Center) ;
  • Suh, Young-Ah (Innovative Cancer Research, Asan Institute for Life Science, Asan Medical Center) ;
  • Kim, Eun-Sung (Graduate School of East-West Medical Science, Kyung Hee University) ;
  • Lee, Young-Min (PGR Assessment & Utilization Lab, National Agrobiodiversity Center (RDA Genebank)) ;
  • Kim, Ye-Seul (Innovative Cancer Research, Asan Institute for Life Science, Asan Medical Center) ;
  • Choi, Eun-Kyung (Innovative Cancer Research, Asan Institute for Life Science, Asan Medical Center) ;
  • Kim, Inki (Asan Institute for Life Science, Asan Medical Center) ;
  • Lee, Sok-Young (PGR Assessment & Utilization Lab, National Agrobiodiversity Center (RDA Genebank)) ;
  • Cho, Dong-Hyung (Graduate School of East-West Medical Science, Kyung Hee University) ;
  • Hong, Nam-Joo (School of Biotechnology, Yeungnam University) ;
  • Kim, Tae-Won (Innovative Cancer Research, Asan Institute for Life Science, Asan Medical Center) ;
  • Jin, Dong-Hoon (Innovative Cancer Research, Asan Institute for Life Science, Asan Medical Center) ;
  • Lee, Wang Jae (Department of Anatomy and Tumor Immunity Medical Research Center, Seoul National University College of Medicine)
  • Published : 2012.12.31
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Abstract

Recently, we reported that an ethanol extract of Iris nertschinskia induces p53-dependent apoptosis in the MCF7 human breast cancer cell line. However, the detailed mechanisms were not fully explored. Here, we demonstrate another aspect of the activity of I. nertschinskia in breast cancer cells. We compared the response to an ethanol extract of I. nertschinskia in two different human breast cancer cell lines, Hs578Tand MDA-MB231, respectively with relatively low and high AKT1/2 activity by trypan blue exclusion assay and FACS analysis. Knockdown of endogenous AKT1 or AKT2 in breast cancer cells by RNA interference determined the sensitivity to I. nertschinskia ethanol extract compared to control cells. The I. nertschinskia ethanol extract induced cell death in a manner that depended on the level of phosphorylated AKT1/2 protein and was associated with a significant increase in the sub-G1 cell population, indicative of apoptosis. Our results indicate that an ethanol extract of I. nertschinskia differentially induces cell death in breast cancer cells depending on their level of phosphorylated AKT1/2.

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References

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