Maxillofacial Plastic and Reconstructive Surgery

Korean Association of Maxillofacial Plastic and Reconstructive Surgeons (대한악안면성형재건외과학회)
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The Effects of Tetracycline-loaded Silk Fibroin Membrane on Guided Bone Regeneration in a Rabbit Calvarial Defect Model

가토 두개골 결손부 모델에서 테트라사이클린 함유 실크 파이브로인 차폐막의 골유도 재생 효과

  • Lee, Sang-Woon (Department of Oral and Maxillofacial Surgery, College of Dentistry, Gangneung-Wonju National University) ;
  • Park, Yong-Tae (Department of Oral and Maxillofacial Surgery, College of Dentistry, Gangneung-Wonju National University) ;
  • Kim, Seong-Gon (Department of Oral and Maxillofacial Surgery, College of Dentistry, Gangneung-Wonju National University) ;
  • Kweon, HaeYong (Sericultural & Apicultural Materials Division, National Academy of Agricultural Science) ;
  • Jo, You-Young (Sericultural & Apicultural Materials Division, National Academy of Agricultural Science) ;
  • Lee, Heui Sam (Sericultural & Apicultural Materials Division, National Academy of Agricultural Science)
  • 이상운 (강릉원주대학교 치과대학 구강악안면외과학교실) ;
  • 박용태 (강릉원주대학교 치과대학 구강악안면외과학교실) ;
  • 김성곤 (강릉원주대학교 치과대학 구강악안면외과학교실) ;
  • 권해용 (농촌진흥청) ;
  • 조유영 (농촌진흥청) ;
  • 이희삼 (농촌진흥청)
  • Received : 2012.07.16
  • Accepted : 2012.08.27
  • Published : 2012.09.30
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Abstract

Purpose: The aim of this study was to evaluate the bone regeneration ability of 1% tetracycline (TC)-loaded silk fibroin membrane (SFM), in a rabbit calvarial defect model. Methods: Twenty New Zealand white rabbits were used for this study. Bilateral round defects were made on the rabbit parietal bone, using trephine bur with an 8 mm diameter. TC-loaded SFM or SFM was covered on the right parietal bone defect, and the left parietal bone defects were uncovered for the control. The animals were humanely sacrificed at 4 or 8 weeks postoperatively. A micro-computerized tomography (${\mu}$-CT) of each specimen was taken for analysis of bone regeneration. Hematoxylin and Eosin stain were done to observe histological findings. Results: From the ${\mu}$-CT results, regenerated bone volume ($mm^3$) of 1% TC-loaded SFM, SFM, and control were $7.80{\pm}5.87$, $8.79{\pm}3.44$, and $10.61{\pm}5.3$ at 4 weeks postoperatively, respectively (P>0.05). Regenerated bone volume ($mm^3$) of 1% TC-loaded SFM, SFM, and control were $36.56{\pm}8.50$, $25.86{\pm}8.17$, and $19.09{\pm}5.07$ at 8 weeks postoperatively, respectively (P<0.05). Conclusion: The 1% TC-loaded SFM showed more bone regeneration than the SFM and the uncovered control, in guided bone regeneration.

Keywords

References

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